30,733 results

1. SQ171fg/pRibo-T

   Plasmid

   #69343

   Purpose

   SQ171 fast growing strain supported by pRibo-T plasmid

   Depositor

   Michael Jewett

   Article

   Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

   Insert

   Ribo-T
   

   Expression

   Bacterial

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZE27GFP3

   Plasmid

   #75456

   Purpose

   Plasmid for constitutive expression of gfp-pdt3

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   gfp-pdt#3
   

   Expression

   Bacterial

   Promoter

   placIq promoter

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEB2-mNeptune2.5

   Plasmid

   #104013

   Purpose

   Plasmid encoding mNeptune2.5

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mNeptune2.5
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (MSKGEE LIKENM… M11…

   Promoter

   proC

   Available Since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSP1D1

   Plasmid

   #20061

   Purpose

   Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1D1
   

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   deletion mutant (1-11) of MSP1; His-tag on N-term…

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-HRas-1-167-WT

   Plasmid

   #245306

   Purpose

   Expresses G-domain of human HRas (residues 1-167 wild type) in E. coli

   Depositor

   Titus Boggon

   Insert

   HRas (HRAS Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   deleted amino acids 168-189

   Promoter

   T7lac

   Available Since

   Oct. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAED2

   Plasmid

   #234599

   Purpose

   Fluorescent reporter of the SOS response

   Depositor

   Tanel Tenson

   Article

   Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):

   Inserts

   mScarlet-I
   gfp-mut2
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   GFPmut2 was derived from avGFP with the following…

   Promoter

   Ptet+dnaK P1 and cda

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRPN265NB-enh-MNase

   Plasmid

   #166035

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

   Insert

   Glutathione-S-Transferase/enhancer/MNase
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   lac promoter / tac promoter

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG/MNase

    Plasmid

    #123461

    Purpose

    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

    Depositor

    Steven Henikoff

    Article

    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

    Insert

    Protein A-Protein G-Micrococcal Nuclease
    

    Tags

    6xHIS and HA

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Expression

    Bacterial

    Mutation

    E32K

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-c4X

    Plasmid

    #75288

    Purpose

    Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 19, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. DJ-1 pET15b

    Plasmid

    #60678

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. EcMscL

    Plasmid

    #92418

    Purpose

    E. coli Mechanosensitive Channel of Large Conductance

    Depositor

    Douglas Rees

    Insert

    MscL (mscL E. coli)
    

    Tags

    6x Histidine Tag and Thrombin Cleavage Site betwe…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEcCas-2.0

    Plasmid

    #190697

    Purpose

    Constitutive expressing of Cas9 and inducible expression of lambda Red

    Depositor

    Sheng Yang

    Article

    Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

    Insert

    Cas9 and Lamda Red system
    

    Expression

    Bacterial

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG1K

    Plasmid

    #71741

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Bacterial

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Constitutive (derepressed P-lac)

    Available Since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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