31,871 results
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Plasmid#58223PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
ExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-TP1107(Q15TAG)
Plasmid#247462PurposeBacterial expression of anti-IgG VHH clone TP1107 with amber stop codon at Q15 for unnatural amino acid incorporationDepositorInsertTP1107 Q15TAG
TagsHis6 and TEV protease cleavage siteExpressionBacterialMutationOriginal Q15 codon (CAA) was modified to amber st…PromoterT7Available SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET15b-12x
Plasmid#61699PurposeExpresses PTDH mutant Opt12xDepositorInsertOpt12x
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKL09-GCaMP6f-mScarlet bb118
Plasmid#158979PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.DepositorInsertGCaMP6f-mScarlet fusion
ExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23118 (sigma 70)Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUB11
Plasmid#169699PurposeTemplate plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
Tags3xFLAG tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
Tags8 histidine tagExpressionBacterialPromoterT7 promotorAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQ-NFY-A-GB-HC
Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorTagsGST and HisExpressionBacterialPromoterT5 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AlkB
Plasmid#228218PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
TagsHis tagExpressionBacterialMutationcodon optimizedPromoterT7Available SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQacR-Q1
Plasmid#74689PurposeQacR-Q1 gateDepositorInsertPTac-RBSQ1-QacR-PQacR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-FL-wt
Plasmid#31621DepositorAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
ExpressionBacterialAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcC)
Plasmid#176197PurposegfcC protein expressionDepositorInsertgfcC
Tags6xHis-TeVExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY71-PT7-RiboJ-sfGFP-MGapt
Plasmid#129119PurposeExpresses a fluorescent reporter for the quantification of cell-free protein expression.DepositorInsertSuperfolder GFP with Malachite Green aptamer
UseSynthetic BiologyTagsHis6 and RiboJ InsulatorExpressionBacterialPromoterT7Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEmal10H – MBP-EWS_300-447 (RGG1+RRM)
Plasmid#238369PurposeExpresses the RGG1-RRM (300-447) region from the RNA-binding protein EWSDepositorInsertEWSR1 (EWSR1 Human)
Tags10xHis-MBPExpressionBacterialMutationdeleted amino acids 1-299, 448-638PromoterT7Available SinceSept. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY18
Plasmid#221708PurposeEmpty Cas9 vector for multiplex gene editing in fungiDepositorTypeEmpty backboneUseCRISPR; AspergillusExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
FDA60
Plasmid#45606DepositorInsertFDA60
Tags6xHisExpressionBacterialAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
T444T
Plasmid#113081PurposeEnhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTn Donor
Plasmid#236211PurposeArabinose-inducible Tn5 InducTn-seq plasmidDepositorInsertNone
ExpressionBacterialAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
TagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)Available SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAP-BAC
Plasmid#120229PurposeTAR cloning in yeast and low-copy maintenance of large constructs in E. coliDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only