32,812 results
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Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
Tags6 his tagExpressionBacterialAvailable SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1B 1-199
Plasmid#115326PurposeExpresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24DepositorAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stapā¦ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOKD4
Plasmid#17214DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT7_SEPT9_i5-TEV-Strep
Plasmid#174502Purposebacterial co-expression of human SEPT7 and of human SEPT9_i5DepositorAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_N
Plasmid#167255PurposeBacterial expression of Nucleocapsid protein (residues 1-419)DepositorInsertN protein (residues 1-419) (N SARS-CoV-2)
TagsHis6-TEV siteExpressionBacterialMutationnoneAvailable SinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AcrIIA2
Plasmid#101042PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA2
TagsGSTExpressionBacterialAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBRα-Ļ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Ļ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
ExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT007-GCN4scFV-VB
Plasmid#163387Purpose2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN systemDepositorInsertantiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH610
Plasmid#131862PurposeProduces holo-PhyB* in E. coliDepositorInsertsPhyB(1-651)-RGD-His6-Cys
HO1
PcyA
ExpressionBacterialPromoterT7 promoter-1 and T7 promoter-2Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 C-terminal half
Plasmid#74599Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523DepositorInserthuman dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialMutationamino acids 389-523 of LIC1PromoterT7Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
4QQG
Plasmid#61948PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP:JMC001-B06:C41570 (KAT5 Human)
Tags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLS.405
Plasmid#187203PurposeExpresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA (wildtype)
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS Wnt-5aHA
Plasmid#17376DepositorAvailable SinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Fast-FT
Plasmid#31916DepositorInsertFast-FT
TagsHisExpressionBacterialMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCherā¦PromoteraraBAvailable SinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCold TDP43 M337V
Plasmid#27453DepositorInsertTDP-43 (TARDBP Human)
TagsFactor Xa and His6ExpressionBacterialMutationMethionine 337 mutated to Valine (M337V)Available SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX Smad3
Plasmid#12630DepositorAvailable SinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pETM33_SUFU_Sufu
Plasmid#178488PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLC290
Plasmid#44447DepositorTypeEmpty backboneUseRk2, pmb1ExpressionBacterialPromoterP_R (Phage 16-3)Available SinceAug. 30, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
TJP2_PDZ_3
Plasmid#103949PurposeProtein expression and purification of X104 PDZ3 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP2_PDZ_2
Plasmid#103955PurposeProtein expression and purification of X104 PDZ2 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-UBE2S-UBD
Plasmid#66713PurposeExpresses the UBE2S-UBD fusion protein in E. coliDepositorAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mouse NHERF-1
Plasmid#32950DepositorAvailable SinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMG023 MBP-Axin 384-518-His (miniAxin)
Plasmid#154012PurposeExpresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
TagsHis6 and MBPExpressionBacterialMutation381-518 fragmentPromoterTacAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease
Plasmid#228637PurposeBacterial expression of the Coxsackievirus 16 3C proteaseDepositorInsertCoxsackievirus 16 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Parp14-c010
Plasmid#173100PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-14-3-3 gamma GST
Plasmid#13280DepositorAvailable SinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMJ824
Plasmid#39314DepositorInsertCas9
UseCRISPRTagsGST and His6ExpressionBacterialPromoterT7Available SinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
CLK1
Plasmid#39149PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only