31,059 results
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Plasmid#216368PurposexhNup155-Nb2i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb2i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC235
Plasmid#216372PurposexhNup155-Nb3i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb3i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC227
Plasmid#216351PurposexhNup35-Nb1t (+3Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSSX-STC
Plasmid#115668PurposeE.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tagDepositorInsertsmall tetraheme cytochrome c
TagsSignal peptide (aa1-27)-Strep-II tag- Factor Xa c…ExpressionBacterialPromotertacAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriex-mVenus-PI-RhoA(Q63L)
Plasmid#86978PurposePI(DM): Photo-activatable/light sensitiveDepositorInsertsTags6xHis and mVenusExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT-9218
Plasmid#124225PurposeBacterial SpCas9 expressionDepositorInsertSpCas9
UseCRISPRExpressionBacterialPromoterTetR/TetAAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3d::strep-AID
Plasmid#15924DepositorAvailable SinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(WT)-IDR-mCherry
Plasmid#145286PurposeBacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion ProteinDepositorInsertRUNX2(WT)-IDR (RUNX2 Human)
ExpressionBacterialAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-Ataxin3Q22
Plasmid#22117DepositorAvailable SinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
CFP-FRBx5-His
Plasmid#103784Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertCFP-FRBx5-His
ExpressionBacterialPromoterT7Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ20
Plasmid#65027PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneTagslacZExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
TagsHis6-SUMO and designed intein CExpressionBacterialPromoterT7Available SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(1-303)IntN
Plasmid#200312PurposeBacterial expression of P53 NTAD-DBD for segmental labelingDepositorInsertp53 (TP53 Human)
TagsHis6GB1tev and designed intein NExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM mouse PEA3 [TJ#200]
Plasmid#16346DepositorInsertPEA3 (Etv4 Mouse)
ExpressionBacterialAvailable SinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv2
Plasmid#221139PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).DepositorInsert3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
UseCRISPRTags3xFLAGExpressionBacterialPromoterlacIq-PtacAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_CVM_N37067PS
Plasmid#224403PurposeFor expression of the type I CBASS system encoded by Escherichia coli CVM N37067PSDepositorInsertEcCdnF_AGS-C_SLATT6
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_BIDMC103
Plasmid#224402PurposeFor expression of the type I CBASS system encoded by Escherichia coli BIDMC103DepositorInsertEcCdnF_AGS-C_SLATT6
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_K71-77
Plasmid#224398PurposeFor expression of the type I CBASS system encoded by Escherichia coli K71-77DepositorInsertPhospholipase_EcCdnE_DisA-like
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_RU1_BHI6
Plasmid#224397PurposeFor expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6DepositorInsertCap15_EcCdnE
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_703128
Plasmid#224394PurposeFor expression of the type I CBASS system encoded by Escherichia coli 703128DepositorInsertEcCdnD_4TM
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_BWH59
Plasmid#224393PurposeFor expression of the type I CBASS system encoded by Escherichia coli BWH59DepositorInsert2TM_EcCdnD
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO4k
Plasmid#210237Purposelow-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPC011
Plasmid#171143PurposeExpression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmidDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
HDAC6 (3GV4)
Plasmid#25297PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHDAC6_42 (HDAC6 Human)
TagsHisExpressionBacterialMutationContains amino acid residues 1109-1215Available SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEX-Antenna-GDI Cdc42
Plasmid#101681Purpose"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et alDepositorInsertCdc42
Tags6xHis and FRET Antenna (mCerulean-cpVenus)ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Q-ACACAA-10
Plasmid#166546PurposescFv of a human scaffold targeting Acetyl-CoA carboxylase 1. Antigen coverage aa 1572 - 2338 of 2346DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
TAF1
Plasmid#39118PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D
Plasmid#159541PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Integrin beta 1b-mKate
Plasmid#105975Purposein vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.DepositorInsertIntegrinbeta1b
TagsmKateExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
XNOR-gate-V2.0
Plasmid#44454DepositorInsertXNOR-gate
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP
Plasmid#108410PurposeMonomeric near-infrared fluorescent protein miRFP in bacterial expression plasmidDepositorInsertmiRFP
ExpressionBacterialPromoteraraBAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBADM11-HMGR
Plasmid#108946Purposeheterologous expression of HMGR of S. solfataricus in E. coliDepositorInsertHMG-CoA reductase (HMGR)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA15_G-alpha
Plasmid#109782PurposeProtein expression and purification of GNA15_G-alphaDepositorInsertGNA15_G-alpha (GNA15 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(YF)-mCherry
Plasmid#87357PurposePI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutationDepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationY535FPromoterCMVAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2x-MT1
Plasmid#32101DepositorAvailable SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHCKAn-phoAp-GRFT
Plasmid#158747Purposelow phosphate phoAp induction of GriffithsinDepositorInsertphoAp Griffithsin
ExpressionBacterialPromoterphoAAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SRpHi3
Plasmid#102543PurposeBacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-sepHluorin
TagsGSTExpressionBacterialPromotertacAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAPA3
Plasmid#24777DepositorTypeEmpty backboneUseIntegrating vectorExpressionBacterialAvailable SinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-TetR-PL22-dCas9-SpR
Plasmid#73223PurposeContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
Tagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPL22Available SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only