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32,812 results

Showing: 7111 - 7140 of 32812 results

  3. pNIC28-SENP1

    Plasmid
    #196354
    Purpose
    protein expression of the His-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    SENP1 catalytic domain
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    contains only E419-L644
    Promoter
    T7
    Available Since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    196354-plasmid-map-sequence-id-386265

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  4. pBlueKan+cysMPro

    Plasmid
    #187879
    Purpose
    Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    CysM
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187879-plasmid-map-sequence-id-368417

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  6. pUX19

    Plasmid
    #36328
    Depositor
    Gary Roberts
    Type
    Empty backbone
    Use
    Suicide vector for r. rubrum
    Expression
    Bacterial
    Promoter
    reduce strength lacZ
    Available Since
    June 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  11. His 3C MKK3 1-347

    Plasmid
    #87878
    Purpose
    E. coli expression of MKK3 aa 1-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-347
    Promoter
    T7
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87878-plasmid-map-sequence-id-174923

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  12. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  14. pOPS0382

    Plasmid
    #133232
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133232-plasmid-map-sequence-id-287534

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  19. R6K_tac_dGFPmut3_ChInt

    Plasmid
    #187388
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187388-plasmid-map-sequence-id-370402

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  24. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid
    #195487
    Purpose
    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    C61T, C262V
    Promoter
    T7
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195487-plasmid-map-sequence-id-393190

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  25. pBAD_mCherry_GFP_-+

    Plasmid
    #187390
    Purpose
    arabinose-inducible expression of GFP and mCherry
    Depositor
    Roi Avraham
    Insert
    GFP, mCherry
    Expression
    Bacterial
    Available Since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187390-plasmid-map-sequence-id-371127

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  27. pM1s3AsR_TS-C7

    Plasmid
    #137945
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available Since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    137945-plasmid-map-sequence-id-331373

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  28. g5s pD42

    Plasmid
    #117452
    Purpose
    Expression of Cyth2
    Depositor
    Richard Kahn
    Insert
    Cyth2 (CYTH2 Human)
    Tags
    V5-His6
    Expression
    Bacterial
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117452-plasmid-map-sequence-id-226811

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Showing: 7111 - 7140 of 32812 results