32,156 results
-
Plasmid#194013PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex3 XTR f1
ExpressionBacterialAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZ001 (AacC2c1 locus)
Plasmid#70166PurposeExpresses AacC2c1 full locusDepositorInsertAacC2c1 locus
ExpressionBacterialAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS1398
Plasmid#205971PurposeMWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1DepositorInsertCas5-HNH crRNA
ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse c-Myc
Plasmid#14972DepositorAvailable SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
Sc eRF3∆165N (WT)
Plasmid#175418PurposeEncodes His-MBP-TEV-WT eRF3∆165NDepositorAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
ExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCTD
Plasmid#157871Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domainDepositorAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-Ataxin3Q22
Plasmid#22117DepositorAvailable SinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
rpoS200-RFP
Plasmid#63840PurposeThis plasmid is designed as a bacterial stationary phase sensorDepositorInsertrpoS, mRFP1 and GFPmut3b
TagsmRFP1ExpressionBacterialMutationGene is translationally fused to RFPAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_N_NTD
Plasmid#156483PurposeBacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tagDepositorInsertN_NTD (N Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Dengue2 NS2B-NS3
Plasmid#204800PurposeBacterial expression of a fusion of Dengue2 NS2B-NS3 proteinsDepositorInsertDengue2 NS2B-NS3 fusion
ExpressionBacterialAvailable SinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP18-Histag_C
Plasmid#127035PurposeBasic Part C- C-terminus; polyhistidine tagDepositorInsertPart His6_C
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SARA-no stop
Plasmid#67347PurposehSara without stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
FtsZ-Dendra2
Plasmid#92038PurposeExpress FtsZ-Dendra2DepositorInsertFtsZ
TagsDendra2ExpressionBacterialAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SIRT5H158Y-pET15b
Plasmid#81032PurposeBacterial expression of Human catalytic mutant SIRT5 proteinDepositorInsertSirt5 (SIRT5 Human)
TagsHisExpressionBacterialMutationH158Y catalytic mutantPromoterT7Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
SIRT6
Plasmid#41564PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD_His B_QuasAr1
Plasmid#64135PurposeFluorescent reporter for membrane voltage with improved brightnessDepositorInsertQuasAr1
ExpressionBacterialAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Q-IL18-6
Plasmid#166554PurposescFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptideDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTn7xKS-dTomato
Plasmid#117395PurposeTn7 tagging vector pTn7xKS with dTomato expression scaffoldDepositorInsertdTomato
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK131
Plasmid#71548PurposeProduces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)DepositorInsertAlanine racemase
ExpressionBacterialPromoterT7Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321G
Plasmid#98670PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321GDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALlR1
Plasmid#172566PurposeInducible gene expression, pACYC origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gfp-rt-vasa
Plasmid#167322PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.DepositorInsertrt vasa 3'UTRs (ddx4 rainbow trout)
ExpressionBacterialAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriex-mVenus-PI-RhoA(Q63L)
Plasmid#86978PurposePI(DM): Photo-activatable/light sensitiveDepositorInsertsTags6xHis and mVenusExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
SRpHi2
Plasmid#102542PurposeBacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-EGFP
TagsGSTExpressionBacterialPromotertacAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1182
Plasmid#205964PurposeNZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1DepositorInsertDinG HNH crRNA
ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1543
Plasmid#205963PurposeNZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)DepositorInsertDinG HNH Cascade with His14-tagged Csf3
Tags14xHis on Csf3ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEX-Antenna-GDI Cdc42
Plasmid#101681Purpose"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et alDepositorInsertCdc42
Tags6xHis and FRET Antenna (mCerulean-cpVenus)ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RBM17_RRM
Plasmid#109786PurposeProtein expression and purification of RBM17_RRMDepositorInsertRBM17_RRM (RBM17 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CSNK1G1
Plasmid#38934PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV co-transformation cloning vector (13K-S)
Plasmid#48321DepositorTypeEmpty backboneTagsHis6 and SumoExpressionBacterialAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_G78R
Plasmid#113047PurposeExpression of mutant hSDH5DepositorAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX-parkin 141-465
Plasmid#45971DepositorInsertParkin 141-465 (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJAK2.D
Plasmid#29465DepositorTypeEmpty backboneUseMycobacteria expressionTags6xHisExpressionBacterialPromoterAcetamide promoterAvailable SinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
PTPN5
Plasmid#39169PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only