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32,167 results

Showing: 11841 - 11880 of 32167 results

  15. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  18. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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  19. 2P0E

    Plasmid
    #60109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. HOXA2 (murine) HIS-tag pET

    Plasmid
    #8557
    Depositor
    Corey Largman
    Insert
    HOXA2 (Hoxa2 Mouse)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    contains 5 additional amino acids from 5' un…
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  30. Stag-YCrs5

    Plasmid
    #215163
    Purpose
    Encodes a yeast cadmium metallothionein fused with the S-tag at the N-terminus
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    Stag-YCrs5
    Tags
    S-tag
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215163-plasmid-map-sequence-id-430158

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  33. NUDT4 (NUDT4A-c001)

    Plasmid
    #98241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT4A (NUDT4 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98241-plasmid-map-sequence-id-193419

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  40. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124807-plasmid-map-sequence-id-251832

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Showing: 11841 - 11880 of 32167 results