30,808 results

1. pEXP5-NT/pT7 LuxR

   Plasmid

   #193625

   Purpose

   Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LuxR
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 RNAP promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-6xHis-(pos28)GFP

   Plasmid

   #89250

   Purpose

   Expresses GFP with +28 theoretical charge in E.Coli

   Depositor

   David Liu

   Insert

   His6-(pos16)-GFP
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET His6 StrepII TEV LIC cloning vector (2HR-T)

   Plasmid

   #29718

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, Strep Ii, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-UBE2S-UBD

   Plasmid

   #66713

   Purpose

   Expresses the UBE2S-UBD fusion protein in E. coli

   Depositor

   David Komander

   Article

   Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

   Inserts

   e2-epf (UBE2S )
   isot (USP5 )
   

   Tags

   GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pSK-mPar1

   Plasmid

   #53227

   Purpose

   bacterial expression of mouse Par1

   Depositor

   Shaun Coughlin

   Insert

   Par1 (F2r Mouse)
   

   Expression

   Bacterial

   Available Since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

6. SNX29-PX (269-391)

   Plasmid

   #119108

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX29-PX (269-391)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRExT2A-CT-BmNG

   Plasmid

   #232207

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Expression

   Bacterial

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRExT2A-CT-PmNG

   Plasmid

   #232201

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Expression

   Bacterial

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM8315

   Plasmid

   #213988

   Purpose

   PrpsM driven expression of mCherry2-L

   Depositor

   Marc Erhardt

   Article

   Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

   Insert

   mCherry2-L
   

   Expression

   Bacterial

   Promoter

   PrpsM

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet1_CylLS_CylM

    Plasmid

    #208760

    Purpose

    Expresses modified cytolysin S (mCylLS) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CylLS
    CylLM
    

    Tags

    Hisx6 Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKFSS1_2

    Plasmid

    #118227

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyCatcher002-oPent

    Plasmid

    #124664

    Purpose

    Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oPent
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGS-21a-PcAad1

    Plasmid

    #163039

    Purpose

    expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

    Depositor

    Jean-Marie Francois

    Article

    Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

    Insert

    aryl-alcohol dehydrogenase
    

    Tags

    GST and His6

    Expression

    Bacterial

    Available Since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTU424

    Plasmid

    #178983

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T HHARI RBR

    Plasmid

    #31254

    Depositor

    Rachel Klevit

    Article

    Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

    Insert

    Human Homologue of Ariadne (HHARI) (ARIH1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    RBR domain residues 177-395

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

17. B4-4

    Plasmid

    #111478

    Purpose

    translational release factor 1

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    translational release factor 1
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMV261-Cas12a

    Plasmid

    #158707

    Purpose

    expresses codon-optimized FnCpf1

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Expression

    Bacterial

    Promoter

    TetO

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-MBP-CBX1-eReader

    Plasmid

    #135089

    Purpose

    Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered
    

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Sac-Abeta(MC1-40)

    Plasmid

    #127152

    Purpose

    Expresses N-terminal cysteine Abeta(1-40)

    Depositor

    James Nowick

    Insert

    Abeta(MC1-40) (APP Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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