-
-
pMJ824
Plasmid#39314DepositorInsertCas9
UseCRISPRTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-EGFP
Plasmid#182641PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
UseTagsBAP, EGFP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-GST-TEV-GG
Plasmid#215801PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDC42
Plasmid#55693Purposeexpression of mouse CDC42DepositorInsertCDC42
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110
Plasmid#135480PurposeE. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXint129
Plasmid#178208PurposeEncodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.DepositorUseTagsExpressionBacterialMutationPromoterlacIAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT1
Plasmid#53557Purposebacterial expression of IFIT1DepositorInsertIFIT1 (IFIT1 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB
Plasmid#110547PurposeCRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.DepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A, H840APromoterpBADAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII12Pglu-700uDelta6
Plasmid#13568DepositorInsertFLIPglu F16A Y12S-ECFP-D13R, 6aa linker
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBPromoterAvailable sinceFeb. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAD-mKate(KPS12)
Plasmid#133831PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPuppAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pTXB1-TnY
Plasmid#163975PurposeIPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.DepositorInsertTnY
UseTagsMxe intein - Chitin-binding domainExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046979
Plasmid#145611PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180156PurposetdTomato cre reporter with Hipp11 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
POLN_pDEST17
Plasmid#64885Purposebacterial expression of POLN for purificationDepositorInsertPOLN (POLN Human)
UseTagsFlag and HisExpressionBacterialMutationPromoterT7Available sinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET co-transformation cloning vector (13S-A)
Plasmid#48323DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pEB2_mScarletI_mVenusNB-MrkHR113A
Plasmid#182292Purposeexpresses c-di-GMP mutant biosensor in E. coliDepositorInsertmVenusNB-MrkHR113A
UseTagsmVenusNB internal fusion to MrkH with a R113A poi…ExpressionBacterialMutationchanged arginine 113 to alanine, MrkH numbering …PromoterproCAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNMT3b KO Neo
Plasmid#16651DepositorInsertDNMT3b knock-out construct (DNMT3B Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only
