29,692 results
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Plasmid#215401PurposeExpression construct for AfNitB gene, codon optimized for expression in E. coliDepositorInsertAfNitB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPC005
Plasmid#171140PurposeFor integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertXylS-Pm
UseCRISPRTagsExpressionBacterialMutationPromoterPmAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN51 (R40A)
Plasmid#80109PurposeLate1-O3-33-myc-PH(R40A)DepositorInsertEPN-51 (R40A)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPA8
Plasmid#177661PurposeRecombinant protein synthesis of human HSPA8DepositorInsertHSPA8 (HSPA8 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG1C
Plasmid#71740PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pλPR cI-Elysis
Plasmid#176891PurposeBacterial expression plasmids for production of E. coli bacterial ghost (BG) capsulesDepositorInsertLysis E gene from PhiX174 and lambda PR-cI857 regulatory system
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE130
Plasmid#118484Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYC-FLAG-dN6-His
Plasmid#22143DepositorInsertFlag-clpXdeltaNLinkedHexamer-His6
UseTagsFlag, His6, and L20 LinkersExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP
Plasmid#166439PurposeBacterial expression of 6x His tagged mEGFPDepositorInsertmEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet30b-5M SrtA
Plasmid#51140Purposeexpression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 5M
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtLeuRS
Plasmid#31079DepositorInsertMature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF184
Plasmid#89482PurposeExpress asparaginyl endopeptidase 1 from O. affinis (OaAEP1)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
UseTagsSmt3ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTP1183
Plasmid#104163PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJS167
Plasmid#48881PurposePositive feedback plasmid from oscillatorDepositorInsertsaraC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialMutationPromoterPara/lacAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJS169
Plasmid#48882PurposeNegative feedback plasmid from oscillatorDepositorInsertlacI with ssrA tag
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialMutationPromoterPara/lacAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-132
Plasmid#118518PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)
Plasmid#159696PurposeE. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)DepositorInsertHis6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
UseTagsHis6-Halotag-tevExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C
Plasmid#159540PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12CDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationG12CPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJ915
Plasmid#69090PurposeExpress Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLSDepositorInsertcas9
UseTagsMBP, hexa His, and two SV40 NLSExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)
Plasmid#110559PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme WT
Plasmid#18110DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#154138PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS_TXS
Plasmid#163839PurposeiPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthaseDepositorInserttaxadiene synthase (61-862)
UseTagsExpressionBacterialMutationPromoterTrcAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Ezr-BioID2-HA
Plasmid#176744PurposeExpresses Ezrin fused with BioID2 at the C-terminus in astrocytesDepositorInsertEzr-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-sfGFP
Plasmid#213863PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z5-Nluc
Plasmid#174487PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ5-Nluc
UseTags6x Histidine tag (N terminal on insert)ExpressionBacterialMutationPromoterT7lac promoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCis GFPuv-SS Himar1 A7
Plasmid#84686PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-luxOFP
Plasmid#229945PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)DepositorInsertluxOFP
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-Venus-pBluescript
Plasmid#166756PurposeFor purification of Venus fluorophoreDepositorInsertVenus
UseTags6x Histidine tag, and TEV protease siteExpressionBacterialMutationPromoterT7Available sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCAT
Plasmid#140084PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHS207 - Bacterial expression plasmid for WT SpCas9
Plasmid#101199PurposeBacterial expression plasmid for WT SpCas9DepositorInsertSpCas9
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pnsgRNA
Plasmid#172717PurposeDelivery of guide RNA for prime editingDepositorInsertJ23119-gRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
UbC-blasticidine-P2A-TETon-3G transactivator
Plasmid#200191PurposeA TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertblasticidine - P2A - TETon-3G transactivator
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterUbCAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJA54 HLA-A*03:01
Plasmid#180451PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*03:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)
Plasmid#159698PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)DepositorInsertHis6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a/Cas9-Cys
Plasmid#53261PurposeExpresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal CysteineDepositorInsertCas9-Cys
UseTags6X His tag, HA epitope, and NLSExpressionBacterialMutationAdditional Cysteine is added to the C-terminal re…PromoterT7 PromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-PTS
Plasmid#108953Purposeheterologous expression of PTS of P. cablin in E. coliDepositorInsertPatchoulol synthase (PTS)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-dhfr
Plasmid#71323PurposeDonor plasmid - trimethoprim resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-Tev
Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK19pA-MN
Plasmid#86973PurposeChIC; genomic mapping of chromatin proteinsDepositorInsertprotein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pChillyBoys_gen1
Plasmid#228495PurposepACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)DepositorInsertsgroEL
groES
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-400_Cacnes_CAMP2
Plasmid#225606PurposeSuicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-RBCS-tagRFP
Plasmid#213868PurposeBinary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertRIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB405-TIC21-sGFP
Plasmid#213865PurposeBinary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .DepositorInsertchloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-mCherry
Plasmid#213862PurposeBinary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused mCherry-UnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only