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32,656 results

Showing: 1651 - 1700 of 32656 results

  4. pMal-Strep

    Plasmid
    #209345
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    209345-plasmid-map-sequence-id-419544

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  7. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid
    #86928
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and td…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    86928-plasmid-map-sequence-id-175058

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  8. GCN5L2

    Plasmid
    #25482
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GCN5L2 (KAT2A Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pMS08

    Plasmid
    #230072
    Purpose
    Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mScarlet-I fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Article
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG and mScarlet-I
    Expression
    Bacterial
    Promoter
    RpoD (bt_1311/BT_RS06635)
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230072-plasmid-map-sequence-id-486154

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  15. pMS06

    Plasmid
    #230071
    Purpose
    Inducible Promoter (rhamnose-induced, BT3763/rhaR system), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Article
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    rhamnose-inducible (BT3763)
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    230071-plasmid-map-sequence-id-476749

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  16. pMS09

    Plasmid
    #230073
    Purpose
    Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal iLOV fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Article
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG and iLOV
    Expression
    Bacterial
    Promoter
    RpoD (bt_1311/BT_RS06635)
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    230073-plasmid-map-sequence-id-475919

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  17. pMS04

    Plasmid
    #230068
    Purpose
    Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Article
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    anhydrotetracycline-inducible
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    230068-plasmid-map-sequence-id-475921

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  19. pBT3043

    Plasmid
    #122546
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::lgg-1 (lgg-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122546-plasmid-map-sequence-id-238140

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  22. pTR47m4

    Plasmid
    #104068
    Purpose
    Luciferase Reporter for Formaldehyde Sensing
    Depositor
    David Liu
    Article
    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.
    Inserts
    xluxAB
    frmR
    frm Promoter m4
    Use
    Luciferase and Synthetic Biology
    Expression
    Bacterial
    Mutation
    23r and 25th nucleotides in sequence mutated from…
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104068-plasmid-map-sequence-id-205132

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  25. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Article
    Sahu et al Nat Protoc. 2025 Mar 3. doi: 10.1038/s41
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    218775-plasmid-map-sequence-id-443448

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  28. YWHAQ

    Plasmid
    #38931
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAQ (YWHAQ Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

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  34. pConRef-2H12.D11

    Plasmid
    #221151
    Purpose
    cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221151-plasmid-map-sequence-id-437168

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  35. pSLC-284

    Plasmid
    #124294
    Purpose
    A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Article
    Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124294-plasmid-map-sequence-id-251247

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  38. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Expression
    Bacterial and Mammalian
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

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  42. pGEX-GST-BcPC-PLC

    Plasmid
    #224255
    Purpose
    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli
    Depositor
    Chiaki Murakami
    Article
    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:
    Insert
    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    Tags
    GST and HRV-3C (LEVLFQGP) site
    Expression
    Bacterial
    Mutation
    The gene of Bacillus cereus ATCC 14579 was used f…
    Promoter
    lac promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    224255-plasmid-map-sequence-id-457146

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  43. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80063
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Sox10 promoter
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80063-plasmid-map-sequence-id-417621

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  46. YARS_pNIC-CTB10H

    Plasmid
    #153059
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    YARS (YARS1 Human)
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153059-plasmid-map-sequence-id-304479

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  47. pcDNA3.1.Gαi3-LgB91

    Plasmid
    #134359
    Purpose
    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi3-LgB91 (GNAI3 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134359-plasmid-map-sequence-id-306392

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Showing: 1651 - 1700 of 32656 results