29,712 results
-
-
pZ8-T_dCas9
Plasmid#74062PurposepZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanRDepositorInsertdcas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBHM2404
Plasmid#173441PurposepRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-NAT1
UseTagsCBP-2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG010 (DRT type 2)
Plasmid#157888PurposeExpresses the DRT type 2 defense systemDepositorInsertDRT type 2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMyNT-kan
Plasmid#84693PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAcetamidaseAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-1β
Plasmid#214340PurposeProtein overexpression in bacteriaDepositorInsertIL1B (IL1B Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCutamp
Plasmid#140632PurposePlasmid-curing in Escherichia coli by targeting the AmpR promoterDepositorInsertSpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-CLIP-tag
Plasmid#167274PurposeOverexpression of CLIP-tag in E. coliDepositorInsertCLIP-tag
UseTagsHis x10ExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-dCas9
Plasmid#218371PurposeExpression of dCas9 for CRISPR-interferenceDepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationPromoterPtetAAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWH1266-Apra-sgRNA
Plasmid#218372PurposesgRNA expression plasmid for CRISPR-intereferenceDepositorInsertsgRNA expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pEN017-SpCas9-hiNLS-t-M4
Plasmid#234455PurposeExpresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGGG-Sr43
Plasmid#186974PurposeSr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).DepositorInsertSr43
UseTagsNoneExpressionBacterial and PlantMutationNonePromoterNaticeAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUM
Plasmid#64176Purposeharbours marker genes URA3 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1
Plasmid#89961PurposeModified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.DepositorInsertCas9HF1
UseTagsExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-Prha-mVenus_rhaS
Plasmid#137662PurposeConjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.DepositorInsertPrha:mVenus
UseTagsExpressionBacterialMutationrhaS (Arg218Leu)PromoterPrhaAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-MCM3/5
Plasmid#116950PurposeExpresses human MCM3 and human MCM5DepositorUseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-FKBPF36V
Plasmid#73180PurposeExpressing FKBPF36V protein in washing out shield-1 ligand for FKBPddDepositorInsertFKBP(F36V) (FKBP1A Human)
UseTagsHis 6xExpressionBacterialMutationF36VPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-mDia1(FH1FH2DAD)-His
Plasmid#85822Purposecontains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.DepositorInsertProtein diaphanous homolog 1 (Diaph1 Mouse)
UseTags8*His and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-pBAD
Plasmid#54531PurposeLocalization: Protein Expression Vector , Excitation: 549, Emission: 565DepositorTypeEmpty backboneUseTags6xHis and mOrange2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mCyRFP1
Plasmid#84545PurposeBacterial expression of mCyRFP1DepositorInsertmCyRFP1
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBHM2406
Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
UseTagsCBP-2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lac-I-SceI-Tet
Plasmid#17655DepositorInsertlac I-SceI tet
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
P25-pZE-SUMO-PbTTA
Plasmid#204634PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-PbTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAD1
Plasmid#47697PurposeGeneral cloning vector for use in E. coli or D. radioduransDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterrepUAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2(+)-zGrad
Plasmid#119716PurposePlasmid to generate zGrad mRNA to inactivate proteins in zebrafishDepositorInsertzGrad
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST HP1 alpha
Plasmid#24074DepositorInsertHP1 alpha (CBX5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#183241Purposeanhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda RedDepositorInsertsgRNA-FRT
UseTagsExpressionBacterialMutationPromoterP-tetAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMON-HsNMT1
Plasmid#67475PurposeExpression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteriaDepositorInsertHsNMT1 (NMT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyDock
Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)
Plasmid#110558PurposeminiTn7 delivery plasmid with lacIq-Ptac inducible promoterDepositorInsertlacIq-Ptac
UseTagsExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-mR7BD
Plasmid#79149Purposebacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)DepositorInsertR7BD (Rilp Mouse)
UseTagsGSTExpressionBacterialMutationamino acids 220-299PromotertacAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2_SEPT6
Plasmid#174497Purposebacterial co-expression of human SEPT2 and of human SEPT6DepositorUseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKL10-mRuby-iATPSnFR1.0 bb118
Plasmid#158980PurposeConstitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.DepositorInsertmruby-iATPSNfR1.0
UseTagsmruby3ExpressionBacterialMutationcodon optimized for e. coli from Addgene#102557PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-Full-Length-MePCE
Plasmid#113539PurposeProduces an N-terminal GST-tagged full length MePCE protein.DepositorInsertMePCE (MEPCE Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZS*12-MS2-GFP
Plasmid#26245DepositorInsertMS2-GFP
UseTagsExpressionBacterialMutationfive amino acid linker (Ser, Gly, Gly, Gly, Gly) …PromoterAvailable sinceNov. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBb(RK2)1k-GFPuv
Plasmid#106394PurposeIPTG-inducible trc promoter expressing gfpuv on RK2 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-LARP7
Plasmid#113545PurposeProduces an N-terminal GST-tagged full length LARP7 protein.DepositorInsertLARP7 (LARP7 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-lldR
Plasmid#74095PurposeL-lactate repressor (LldR) from E. coli MG1655DepositorInsertlldR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG024 (SIR2 + HerA)
Plasmid#157902PurposeExpresses the SIR2 + HerA defense systemDepositorInsertSIR2 + HerA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKL003
Plasmid#98919PurposeConstitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.DepositorInsertPROPS
UseTagsHisExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFW2500
Plasmid#224213PurposeBacteroides genomic editing tool; IPTG inducible-Fncpf1 and recTDepositorInsertFncpf1 and recT
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23109
Plasmid#113151PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A & H840APromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mScarlet-I
Plasmid#163756PurposeExpresses Streptomyces codon-optimised mScarlet-IDepositorInsertmScarlet-I
UseSynthetic BiologyTagsFlag and FlAsH tagsExpressionBacterialMutationPromoterSP44Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP1a
Plasmid#205304PurposeExpression of WHaloCaMP1a in E. coliDepositorInsertWHaloCaMP1a
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-km
Plasmid#117235PurposeA cytidine deaminase-mediated base-editing plasmid in Klebsiella PneumoniaeDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only
