32,069 results
-
Plasmid#186332PurposeThis plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.DepositorInsertvirulence genes from Agrobacterium tumefaciens pTiBo542
ExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEA106
Plasmid#187872PurposeExpresses mCherry under the virB promoter.DepositorInsertmCherry
ExpressionBacterialPromoterAgrobacterium virB promoterAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-mDia1(FH1FH2DAD)-His
Plasmid#85822Purposecontains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.DepositorInsertProtein diaphanous homolog 1 (Diaph1 Mouse)
Tags8*His and GSTExpressionBacterialPromotertacAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI
Plasmid#115219PurposeExpresses PspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPspCas13
TagsSUMOExpressionBacterialAvailable SinceSept. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-SpyTagMBP
Plasmid#35050DepositorInsertSpyTagMBP
TagsHis6ExpressionBacterialMutationSimple peptide tag added to the N-terminus of MBP…PromoterT7 promoterAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-NT
Plasmid#164861PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-NT
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBinit
Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneTagsmyc, HisExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-pBAD
Plasmid#54553PurposeLocalization: Protein Expression Vector , Excitation: 462, Emission: 492DepositorTypeEmpty backboneTags6xHis and mTFP1ExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM5411
Plasmid#132663PurposeRSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-HIV RT-6xHis-RBS-prot
Plasmid#159149Purposeexpresses His-tagged HIV reverse transcriptase and untagged HIV proteaseDepositorInsertsHuman immunodeficiency virus 1 (HIV-1) reverse transcriptase
Human immunodeficiency virus 1 (HIV-1) protease
TagsHisExpressionBacterialMutationHIV-1 group M/subtype B – BH10 strainAvailable SinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p357_DG
Plasmid#234700PurposeGolden gate domesticated pET28b for scarless cloning with His10-GST-3CDepositorTypeEmpty backboneTagsHis10-GST-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p353_DG
Plasmid#234696PurposeGolden gate domesticated pET28b for scarless cloning with His10-3CDepositorTypeEmpty backboneTagsHis10-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
cwby0082
Plasmid#247432PurposeGGA cloning AGGA-H6-GS-promoter-ccdb-TTCC-SNAC-6xhisDepositorInsertccdb
ExpressionBacterialPromoterT7Available SinceNov. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-ATG41
Plasmid#113080PurposeContain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.DepositorInsertATG41
ExpressionBacterial and YeastPromoterATG41 endogenous promoterAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDG04439
Plasmid#216365PurposexhNup133-Nb2t (+2Cys) Nanobody productionDepositorInsertxhNup133-Nb2t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
NW11
Plasmid#133443PurposeCovalently circularized nanodisc NW11 for studying membrane proteins and viral entry.DepositorInsertNW11
ExpressionBacterialAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTET GFP10-FRB/FKBP-GFP11
Plasmid#182497PurposeTripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coilsDepositorInsertsTagssplit GFP10 tripartite and split GFP11 tripartiteExpressionBacterialPromoterTETAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
Tags8xHIS and BPNLSExpressionBacterialPromoterpRhaBADAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-mStayGold (E138D)
Plasmid#211361PurposeBacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
Tags10xHis-tagExpressionBacterialMutationE138DPromoterT7Available SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb713-HO
Plasmid#141199PurposeExpresses mRhubarb713 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb713
heme oxygenase
Tags6x-HisExpressionBacterialMutationR134H, W309R, Q310A compared to iRFPPromoterT7Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pADsacA
Plasmid#158649PurposeConstitutive transcription of sacA crRNA and GFPDepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…ExpressionBacterialPromoterPvegAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
TagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
TagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
TagsbdSUMOExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
ExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDuBir-Lbu-dCas13a-avitag
Plasmid#100817PurposeDual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13aDepositorInsertLbu_Cas13a_R472A_H477A_R1048A_H1053A
TagsAviTag and His6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterT7Available SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-TAG150
Plasmid#212121PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertssfGFP-150TAG
M. alvus Pyl-tRNA(6)
TagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK-Ma pylRS nitroY/haloY-F5
Plasmid#212125PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS 3-NY/HaloY F5
ExpressionBacterialMutationCTG125CTT, N166A V168S, A223C, W239RPromoterGlnS (constitutive)Available SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only