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32,069 results

Showing: 1411 - 1440 of 32069 results

  3. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  8. pTP1174

    Plasmid
    #104162
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104162-plasmid-map-sequence-id-200079

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  9. p5E-2.3k-fabp2

    Plasmid
    #84927
    Purpose
    –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector
    Depositor
    John Rawls
    Insert
    –2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
    Tags
    NO
    Expression
    Bacterial
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    84927-plasmid-map-sequence-id-254043

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  13. pAIP4_5Y33

    Plasmid
    #234204
    Purpose
    Heterologous protein expression of alginate lyase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5Y33
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234204-plasmid-map-sequence-id-464169

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  19. SUMO-PNGaseF

    Plasmid
    #228498
    Purpose
    10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    ngl
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228498-plasmid-map-sequence-id-455750

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  20. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100023-plasmid-map-sequence-id-194290

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  24. pGEX6P1-N-Myc

    Plasmid
    #119757
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Myc
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119757-plasmid-map-sequence-id-233668

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  27. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid
    #225617
    Purpose
    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225617-plasmid-map-sequence-id-451418

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  28. pExp-Sumo

    Plasmid
    #112574
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112574-plasmid-map-sequence-id-217243

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Showing: 1411 - 1440 of 32069 results