30,808 results

1. pH6HTC_CCDC59-Halo

   Plasmid

   #175327

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCDC59 (CCDC59 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. T5-sgRNA

   Plasmid

   #113655

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter T5 and sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SMS

   Plasmid

   #25234

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SMS_01
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Dec. 21, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pET15b/BOA

   Plasmid

   #82041

   Purpose

   For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

   Depositor

   Angela Gronenborn

   Article

   Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

   Insert

   Burkholderia oklahomensis agglutinin
   

   Tags

   6x His

   Expression

   Bacterial

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TOPO tTR-KRAB-rtTA3-Luc

   Plasmid

   #68449

   Purpose

   TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   

   Use

   Luciferase; Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINO_C1

   Plasmid

   #171924

   Purpose

   Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

   Insert

   VHH_C1
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Sept. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBLxR5

   Plasmid

   #167504

   Purpose

   Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLuxB

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWH-DI

   Plasmid

   #47385

   Purpose

   Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Inserts

   dxs
   idi
   

   Expression

   Bacterial

   Promoter

   PxylA

   Available Since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pMOD3-mWasabi-nat

   Plasmid

   #120331

   Purpose

   Bacterial mWasabi promoter trap

   Depositor

   James Kirby

   Article

   Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

   Insert

   mWasabi
   

   Expression

   Bacterial

   Promoter

   (None)

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH6HTC_hsHUR-Halo

    Plasmid

    #175303

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HUR (ELAVL1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. C331

    Plasmid

    #141123

    Purpose

    RhlI expressing plasmid

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Insert

    RhlI (rhlI )
    

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    engineered lac promoter

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HS89 pComb3xss anti-Homer1 nanobody

    Plasmid

    #134717

    Purpose

    Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))
    

    Tags

    6xHis-HA

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCJ209

    Plasmid

    #162682

    Purpose

    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Replacement of G251-T472 with W185-F191 from PETa…

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NKTR

    Plasmid

    #25597

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NKTR (NKTR Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. METT10D

    Plasmid

    #25485

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METT10D (METTL16 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pFAB815

    Plasmid

    #47854

    Purpose

    BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    rnpB T1 terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO9

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE-60NA-mScarlet-2I

    Plasmid

    #160463

    Purpose

    Encodes a variant of mScarlet-I that displays reduced cytotoxicity in bacteria

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    mScarlet-2I
    

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKS014

    Plasmid

    #65467

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    Ntag-beta galactosidase
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    pl-TetO

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pGEX Slp76-SH2

    Plasmid

    #46503

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Slp76 (LCP2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 417-533

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC-EsaR-EsaI

    Plasmid

    #47660

    Purpose

    esaI gene downstream of esaR in pAC-EsaR (CmR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    esaI-term
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only