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32,167 results

Showing: 201 - 240 of 32167 results

  3. pmr101A-cPr-mCherry (KmR)

    Plasmid
    #177207
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177207-plasmid-map-sequence-id-348603

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  4. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Expression
    Bacterial
    Promoter
    AmpR and lpp
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

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  5. pMBP-hLIF

    Plasmid
    #179711
    Purpose
    Expresses H6-MBP-LIF
    Depositor
    Mark Arbing
    Insert
    Leukemia inhibitory factor (LIF Human)
    Tags
    His6-MBP-TEV protease site
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179711-plasmid-map-sequence-id-352198

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  7. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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  14. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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  17. pOPINE-3C-eGFP

    Plasmid
    #41125
    Depositor
    Ray Owens
    Insert
    GFP
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41125-plasmid-map-sequence-id-54698

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  18. pHN767

    Plasmid
    #178884
    Purpose
    Expression of bacterial PNGase
    Depositor
    Kazuhiro Abe
    Insert
    Peptide:N-glycosidase F
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178884-plasmid-map-sequence-id-354970

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  19. pM1s3ATScsg-Etag

    Plasmid
    #137946
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    Synthetic curli operon
    Synthetic curli operon
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    pTlpA36
    Available Since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    137946-plasmid-map-sequence-id-331396

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  25. pOPINEneo-3C-GFP

    Plasmid
    #53534
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Tags
    GFP-His8
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53534-plasmid-map-sequence-id-83411

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  26. L57V

    Plasmid
    #34556
    Depositor
    Kevin Truong
    Insert
    L57 (CASP7 A.Sativa (LOV2), Human)
    Tags
    YFP
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic domain of caspase-7 was truncated at am…
    Promoter
    CMV, T7
    Available Since
    April 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  30. pET28-Strep-TEV-Nsp14

    Plasmid
    #154955
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    Strep-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154955-plasmid-map-sequence-id-307850

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Showing: 201 - 240 of 32167 results