30,888 results

1. pET28a-EGFP-CNA35

   Plasmid

   #61603

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with EGFP

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   EGFP and CNA35
   

   Tags

   His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNF02-mLemon

   Plasmid

   #219399

   Purpose

   Constitutive expression of mLemon in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mLemon
   

   Expression

   Bacterial

   Promoter

   proDp

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-Bxb1-His

   Plasmid

   #123132

   Purpose

   Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Bxb1 integrase
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pWH1266-Apra-sgRNA

   Plasmid

   #218372

   Purpose

   sgRNA expression plasmid for CRISPR-intereference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   sgRNA expression cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL4.R_TRE_minCMV

   Plasmid

   #211518

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   SV40 and minCMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNF02-mJuniper

   Plasmid

   #219398

   Purpose

   Constitutive expression of mJuniper in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mJuniper
   

   Expression

   Bacterial

   Promoter

   proDp

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSIBR-Int4-Cas9-NT

   Plasmid

   #235166

   Purpose

   Expresses the SIBR-Int4-Cas9 system and a NT sgRNA

   Depositor

   Constantinos Patinios

   Article

   Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

   Insert

   SIBR-Int4-Cas9
   

   Expression

   Bacterial

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAW016-2

   Plasmid

   #85613

   Purpose

   Cas9-tracrRNA integration vector for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   cas9-tracrRNA
   

   Expression

   Bacterial

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-p300

    Plasmid

    #191764

    Purpose

    Expression of EGFP fused to core p300 histone acetyltransferase

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    FRB-EGFP-p300 core (EP300 Human)
    

    Tags

    EGFP (N-terminal of p300 core) and FRB (N-termina…

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-mCherry-hphMX6

    Plasmid

    #105156

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    mCherry-hphMX6
    

    Tags

    mCherry

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW005-2

    Plasmid

    #85611

    Purpose

    Empty gRNA delivery vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEM-Sox2

    Plasmid

    #16353

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-SENP1 (catalytic domain)

    Plasmid

    #16356

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP1 catalytic domain (SENP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 419 to 644 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His6-EGFP-PH-PLCD1

    Plasmid

    #183675

    Purpose

    PtdIns(4,5)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLCD1 (PLCD1 Human)
    

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 11-140

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His-MBP_G4G6 in pRSF

    Plasmid

    #188454

    Purpose

    Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    G4G6 (GSN Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJ1996_v2

    Plasmid

    #140664

    Purpose

    dCas9 & Csy4

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    dCas9 & Csy4
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD-pHlorina

    Plasmid

    #182763

    Purpose

    pH-sensitive RFP pHlorina for detection of efferocytosis

    Depositor

    Robert Campbell

    Article

    Raymond et al Science. 2022 Mar 11;375(6585):1182-1187

    Insert

    pHlorina
    

    Expression

    Bacterial

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge