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32,069 results

Showing: 3041 - 3080 of 32069 results

  1. pCarCBADE_ProB(I69E)A

    Plasmid
    #117756
    Purpose
    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    117756-plasmid-map-sequence-id-230175

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  4. IRES-creERT2-FNF

    Plasmid
    #188230
    Purpose
    EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
    Depositor
    Mario Capecchi
    Insert
    IRES-creERT2-FNF
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    188230-plasmid-map-sequence-id-371215

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  8. pFV2-HA

    Plasmid
    #187082
    Purpose
    Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)
    Depositor
    Steven Vik
    Article
    Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1
    Insert
    atpBEFHAGDC
    Tags
    6-His beta subunit (atpD) and HA tag subunit a (a…
    Expression
    Bacterial
    Mutation
    All Cys codons changed to Ala.
    Available Since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    187082-plasmid-map-sequence-id-372620

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  15. pHUE-eGFP-Clu-tails

    Plasmid
    #215397
    Purpose
    Expression of a His6-Ubiquitin-Clu(228-238)-eGFP-Clu(204-227) fusion protein
    Depositor
    Franz-Ulrich Hartl
    Insert
    Clu(228-238)-eGFP-Clu(204-227) (CLU Human)
    Tags
    His6-Ubiquitin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215397-plasmid-map-sequence-id-430524

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  17. pCDM4-GLY

    Plasmid
    #49808
    Purpose
    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49808-plasmid-map-sequence-id-74623

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  22. pMAL-p5X

    Plasmid
    #150814
    Purpose
    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein
    Depositor
    Paul Riggs
    Type
    Empty backbone
    Tags
    malE A313V
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 22, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    150814-plasmid-map-sequence-id-306372

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  23. pCW-LIC

    Plasmid
    #26098
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Three consecutive Taq promoters, 2 active
    Expression
    Bacterial
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26098-plasmid-map-sequence-id-251071

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  24. pQE81L-KOFP7

    Plasmid
    #160330
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2020 May 27;68(21):58
    Insert
    KOFP7
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160330-plasmid-map-sequence-id-326602

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  26. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

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  27. BK Dunlop

    Plasmid
    #25466
    Depositor
    Peter Howley
    Insert
    human papovavirus BK Dunlop
    Expression
    Bacterial
    Available Since
    Sept. 26, 2011
    Availability
    Academic Institutions and Nonprofits only

  28. pEF1-Pls1-sfGFP

    Plasmid
    #205748
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls1 (Phyperspank mutant, IPTG inducible)
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    205748-plasmid-map-sequence-id-483889

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  29. pDEST-N2H-N1

    Plasmid
    #125547
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to n-terminus of Gatew…
    Expression
    Bacterial and Mammalian
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125547-plasmid-map-sequence-id-254706

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  32. NEK1A-c011

    Plasmid
    #53661
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    NEK1A
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. pHJL1

    Plasmid
    #199376
    Purpose
    Encodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6s::SL2::mScarlet-I
    Expression
    Bacterial
    Mutation
    no mutations
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199376-plasmid-map-sequence-id-390952

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  36. pETM6-FAS

    Plasmid
    #49810
    Purpose
    Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    fatty acyl-ACP thioesterase
    hydroxydecanoyl-ACP dehydratase
    b-ketoacyl-ACP synthase III
    3-ketoacyl-ACP reductase
    enoyl-(acyl carrier protein) reductase
    Expression
    Bacterial
    Mutation
    Codon-optmized. See supplemental information of …
    Promoter
    T7
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49810-plasmid-map-sequence-id-74625

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  37. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive native promoters
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65006-plasmid-map-sequence-id-109903

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  40. pKM402

    Plasmid
    #107770
    Purpose
    Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria
    Depositor
    Kenan Murphy
    Article
    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:
    Inserts
    Che9C RecT
    TetR (tet repressor)
    cat
    kan
    Expression
    Bacterial
    Promoter
    Cat promoter, Ptet, kan promoter, and pTB21
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107770-plasmid-map-sequence-id-210549

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Showing: 3041 - 3080 of 32069 results