30,535 results

1. pYTRW09K_0T5

   Plasmid

   #177281

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   Ptet-tetA(C)
   

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPN265NB-LaG16-MNase

   Plasmid

   #170978

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

   Insert

   Glutathione-S-Transferase/LaG16/MNase
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEXTK-Pak1 70-117

   Plasmid

   #12217

   Depositor

   Jonathan Chernoff

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PBD (p21-binding domain), aa 70-117

   Promoter

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTHSSe_60

   Plasmid

   #109254

   Purpose

   TALEsp2 stabilized promoter driving GFP

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TALEsp2 stabilized promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15TV-L-AldA WT

   Plasmid

   #187976

   Purpose

   Expresses human aldolase A

   Depositor

   Audrey Lamb

   Article

   Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

   Insert

   AldA WT (ALDOA Human)
   

   Use

   Tags

   Hexahistag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTHSSe_59

   Plasmid

   #109253

   Purpose

   TALEsp1 stabilized promoter driving GFP

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TALEsp1 stabilized promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. NylCK-TS_pET28a

   Plasmid

   #215423

   Purpose

   Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S111G/A137L

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-NlaCascade-NLS-6xHis

   Plasmid

   #180213

   Purpose

   Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   NlaCas5-Cas8-Cas7
   

   Use

   Tags

   6XHis and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-MNase

   Plasmid

   #136291

   Purpose

   Expresses Micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAWP78-PVCpnf_pvc13-antiHistoneNb

    Plasmid

    #198293

    Purpose

    Produces PVCs retargeted with A9 anti-histone nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiHistoneNb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. NowGFP /pQE-30

    Plasmid

    #74749

    Purpose

    NowGFP gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

    Insert

    NowGFP
    

    Use

    Tags

    HisTag (with a mutation but still working)

    Expression

    Bacterial

    Mutation

    WasCFP with substitutions E6K, L42M, T43S, V68M, …

    Promoter

    T5

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. bdSUMO-GFP-HiBit

    Plasmid

    #170989

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAKgfp1

    Plasmid

    #14076

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pDonor-GDH

    Plasmid

    #140629

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    Mini-Tn6677, T7lac-GDH gene cassette
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTnsABC

    Plasmid

    #160031

    Purpose

    Expresses V. cholerae TnsABC

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    no

    Promoter

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTHSSe_42

    Plasmid

    #109240

    Purpose

    pSC101 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCali2

    Plasmid

    #86119

    Purpose

    Multiplex temperature-gated expression of mWasabi and mCherry reporters

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA36
    mWasabi
    TcI
    mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T and D341A, P60L, D135V, K187R, K202I, L208Q

    Promoter

    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM4Z-Fluc

    Plasmid

    #183474

    Purpose

    This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Sac-Abeta(M1-40)

    Plasmid

    #71876

    Purpose

    Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-40) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge