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Showing: 141 - 160 of 29495 results

  3. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Guillaume Romet-Lemonne, Antoine Jégou
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Use
    Tags
    8*His and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  4. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  6. NUDT16A

    Plasmid
    #42404
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT16A (NUDT16 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pBDC

    Plasmid
    #135188
    Purpose
    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    CmR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    cat
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135188-plasmid-map-sequence-id-273773

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  14. APRT

    Plasmid
    #64028
    Purpose
    Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.
    Depositor
    Vern Schramm
    Article
    Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9
    Insert
    adenine phosphoribosyltransferase (APT1 Budding Yeast)
    Use
    Tags
    Histidine x 6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. PARP2-c098

    Plasmid
    #216873
    Purpose
    Bacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusion
    Depositor
    Lari Lehtiö
    Article
    Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10
    Insert
    PARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216873-plasmid-map-sequence-id-434366

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  19. pAR100

    Plasmid
    #180377
    Purpose
    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).
    Depositor
    Alec Redwood
    Article
    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:
    Inserts
    NFAT-Luciferase
    hCD8A
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    hEF1-HTLV and minimal promoter and an NFAT respon…
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    180377-plasmid-map-sequence-id-370772

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Showing: 141 - 160 of 29495 results