30,535 results

1. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBbS5c-mCherry-AsLOV2*(543)

   Plasmid

   #202712

   Purpose

   Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

   Depositor

   Mary Dunlop

   Article

   Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

   Insert

   mCherry-AsLOV2*(543)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC28-LIC-sfGFP-His

   Plasmid

   #165479

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

   Depositor

   Renato Bruni

   Article

   Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

   Type

   Empty backbone

   Use

   Tags

   TEV-GFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB167

   Plasmid

   #101672

   Purpose

   Opto-T7RNAP*(563), equal expr.

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563), equal expr.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-MinE-His

   Plasmid

   #133623

   Purpose

   Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

   Insert

   MinE-His (minE E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YL-CashGem-BAT1-URA3-LINEAR

   Plasmid

   #174837

   Purpose

   pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GPDp

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-human LIC1 C-terminal half

   Plasmid

   #74599

   Purpose

   used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
   

   Use

   Tags

   N-terminal GST and Strep II

   Expression

   Bacterial

   Mutation

   amino acids 389-523 of LIC1

   Promoter

   T7

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTYB2-eIF1A

   Plasmid

   #37218

   Depositor

   Jon Lorsch

   Article

   Acker et al Methods Enzymol. 2007;430:111-45.

   Insert

   eIF1A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

9. p404GALS

   Plasmid

   #17435

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GALS promoter is between SacI and XbaI restrictio…

   Promoter

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD18HMGR

    Plasmid

    #19041

    Depositor

    Jay Keasling

    Article

    Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

    Insert

    truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pEF1-Pls4-sfGFP

    Plasmid

    #205751

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pls4 (Phyperspank mutant, IPTG inducible)

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKEK3230

    Plasmid

    #234102

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    FuGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBad33_IS605_reRNA

    Plasmid

    #205321

    Purpose

    Bacterial expression of IS605 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    IS605 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTXB1-Tn5

    Plasmid

    #60240

    Purpose

    IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

    Depositor

    Rickard Sandberg

    Article

    Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

    Insert

    tnpA from pBam1
    

    Use

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7 promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. tFucci(CA)5

    Plasmid

    #153521

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(CA)5.
    

    Use

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTNS2

    Plasmid

    #64968

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSC101-AmpR EV

    Plasmid

    #208184

    Purpose

    empty plasmid vector, non-fluorescent

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Type

    Empty backbone

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge