31,059 results
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Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorExpressionBacterialPromoterT7 promoterAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
Tags3xFlag and intein-CBDExpressionBacterialPromoterT7Available SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB150
Plasmid#101661PurposeOpto-T7RNAP*(563)DepositorInsertOpto-T7RNAP*(563)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET21a-C2-SNAP
Plasmid#208769PurposeTo express C2-SNAP in bacteriaDepositorAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO_LPETG_MT3
Plasmid#200304PurposeBacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavageDepositorAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-PRM-B0034-geneVI
Plasmid#80858PurposeAccessory plasmid (encodes M13 gene VI)DepositorInsertM13 gene VI
ExpressionBacterialPromoterLambda PrmAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM1773-PRHIII-sfGFP-pNW33N
Plasmid#52217PurposeConstitutive sfGFP expression plasmid for use in Geobacillus and E. coliDepositorInsertPRHIII-sfGFP
ExpressionBacterialPromoterPRHIIIAvailable SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFW2000
Plasmid#224211PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyo094 (eMutaT7)
Plasmid#173124PurposeExpresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertpmCDA1-T7RNAP
ExpressionBacterialAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
TagsMet-6xHisExpressionBacterialPromoterT5 promoterAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWSK29
Plasmid#172972PurposeLow copy number cloning vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-GFPplus
Plasmid#172718PurposeExpression of a fast folding GFP variant GFP plusDepositorInsertGFP plus
ExpressionBacterialAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
S96 ELP
Plasmid#68393PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residueDepositorInsertS96 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
plRL118
Plasmid#163632PurposeRenilla luciferase reporter plasmid; Giles integrating.DepositorTypeEmpty backboneUseLuciferaseExpressionBacterialPromoterMycobacterial Optimized Promoter (MOP)Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP566
Plasmid#222724PurposepNP561 - IS621 WT Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-1b-RFC140ΔN555
Plasmid#175043PurposeOverexpression of human (Rfc1) p140ΔN555 in E.coliDepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Human, Synthetic)
TagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNG
Plasmid#203675PurposepQFT with mNeonGreen under control of PQJDepositorInsertmNeonGreen
ExpressionBacterialPromoterPQJAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only