30,738 results

1. pGEX-6P-1 Stu2 1-590

   Plasmid

   #38314

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDJSVT87

   Plasmid

   #109302

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Tags

   OmpA 1-27, 6His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX4T-1-Optimized UBE1L

   Plasmid

   #165099

   Purpose

   ecoli optimized expression of GST-fusion of human Ube1L

   Depositor

   Arthur Haas

   Insert

   UbeE1L (UBA7 Human)
   

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGLO-Blue

   Plasmid

   #220782

   Purpose

   Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   blue chromoprotein (aeBlue)
   

   Tags

   8xHis

   Expression

   Bacterial

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAL-SH3(2)-5R

   Plasmid

   #112089

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-5R (NCK1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only repeats of the second SH3…

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNuc-cis

   Plasmid

   #202796

   Purpose

   RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

   Depositor

   David Edgell

   Article

   Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

   Insert

   TevSpCas9, sgRNA cassette, oriT, CenArsHis
   

   Expression

   Bacterial

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-D2

   Plasmid

   #37470

   Depositor

   Amy Palmer, Roger Tsien

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D2 second generation cameleon (calcium sensor)
   

   Expression

   Bacterial

   Promoter

   Ara

   Available Since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pMal-Abl-PRM 5R

   Plasmid

   #112088

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   PRM-5R (ABL1 Human)
   

   Tags

   His-6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only the PRM motif of Abl1

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCL149

   Plasmid

   #134457

   Purpose

   CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.

   Depositor

   Thomas Bernhardt

   Article

   Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

   Insert

   Para-popZ-rbs-H3H4-msfGFPN-CTM.
   

   Expression

   Bacterial

   Promoter

   Para

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUD-MSP1D1

    Plasmid

    #161608

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans and tyrosines w…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMG046 pETARA-CK1α-His

    Plasmid

    #154074

    Purpose

    Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-KIRIN1

    Plasmid

    #107414

    Purpose

    Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Expression

    Bacterial

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbA5c-RFP

    Plasmid

    #35281

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid

    #154072

    Purpose

    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Inserts

    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    

    Tags

    GST, His6, and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28HIS-hAPE1

    Plasmid

    #70757

    Purpose

    bacterial expression of human APE1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    APEX1 (APEX1 Human)
    

    Tags

    6-HIS

    Expression

    Bacterial

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGRG25

    Plasmid

    #16665

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSiMPlk_N + pSiMPlk_C

    Plasmid

    #134310

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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