31,059 results
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Plasmid#73244PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAAK1 (AAK1 Human)
TagsTEV-His6-FlagExpressionBacterialMutationpfam00069:Pkinase 47-307PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-mCherry-hPMCA2w/b
Plasmid#111570PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-6AlanineHE1
Plasmid#165098Purposebacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1DepositorAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKEW303-AcrVA5
Plasmid#115657PurposeExpresses AcrVA5 in bacteria, under TetR control.DepositorInsertAcrVA5
UseSynthetic BiologyExpressionBacterialPromoterPtetAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1mCNPRS
Plasmid#174718PurposetRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DYNLL1_Dynein-light
Plasmid#110006PurposeProtein expression and purification of DYNLL1_Dynein-lightDepositorInsertDYNLL1_Dynein-light (DYNLL1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14107 fwYellow-targeting sgRNA
Plasmid#239456Purposeguide for fwYellowDepositorInsertguide targeting fwYellow
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ3533 eforRed
Plasmid#239451Purposeexpress eforRed chromoproteinDepositorInserteforRed
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ3534 fwYellow
Plasmid#239452Purposeexpress fwYellow chromoproteinDepositorInsertfwYellow
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14112 tyrosinase-targeting sgRNA
Plasmid#239751Purposeguide for tyrosinaseDepositorInsertguide targeting tyrosinase
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14103 aeBlue-targeting_sgRNA
Plasmid#239750Purposeguide for aeBlueDepositorInsertguide targeting aeBlue
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAT112TAG-SepT
Plasmid#34624DepositorInserttRNA^Sep
ExpressionBacterialPromoterlppAvailable SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJ841
Plasmid#39318DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterT7Available SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2
Plasmid#174491Purposebacterial expression of human SEPT2DepositorAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LacI
Plasmid#46394PurposeOverproduction of LacIDepositorInsertLacI
Tags6xHIS tagExpressionBacterialMutationsilent T to C mutation at position 19Promoterarabinose promoterAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMH48
Plasmid#131861PurposeProduces holo-Cph1* in E. coliDepositorInsertsCph1(1-514,Y263F)-RGD-His6-Cys
HO1
PcyA
ExpressionBacterialPromoterT7 promoter-1 and T7 promoter-2Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAPK1
Plasmid#73248PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMJ826
Plasmid#39316DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationHistidine 840 to Alanine (H840A)PromoterT7Available SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMDH
Plasmid#133129PurposeA recombinant plasmid expressing mdh (malate dehydrogenase) geneDepositorInsertmalate dehydrogenase gene
ExpressionBacterialPromotermalate dehydrogenase promoterAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK1010 (pSL25)
Plasmid#191005PurposeExpresses blue chromoprotein on a broad host range RP4 origin plasmidDepositorInsertblue chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLQ-Pxyl/tet-cas9-Pj23119-sgRNA
Plasmid#92121PurposeCRISPR-Cas9 based efficient genome editing in S. aureusDepositorInsertCas9-Pxyltet-sgRNA-pj23119
UseCRISPRExpressionBacterialAvailable SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF RBP-FL P53
Plasmid#174042PurposeExpress Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)DepositorInsertP53 gene (TP53 Human)
TagsThermoanaerobacter Tencongenesis ribose binding p…ExpressionBacterialPromoterT7Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-160
Plasmid#118540PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI2_G-alpha
Plasmid#109791PurposeProtein expression and purification of GNAI2_G-alphaDepositorInsertGNAI2_G-alpha (GNAI2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-mKelly2
Plasmid#119783PurposeExpresses mKelly2 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmKelly2
Tags6xHis tagExpressionBacterialPromoterT7Available SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-FlicR1
Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
ExpressionBacterial and MammalianPromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKSJ376
Plasmid#103065PurposeExpresses colicin E1 with R domain deletion and ImmE1DepositorExpressionBacterialMutationR (receptor-binding domain) deletion in colicin E…PromoterT7 lac (from pET) and T7 lac (from pET52-b)Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI
Plasmid#117006PurposeExpresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
TagsHis and MBPExpressionBacterialAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYBB1
Plasmid#217675PurposeFor expression of SUMO tagged human beta B1 crystallinDepositorAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD11
Plasmid#31333DepositorInsertproteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
ExpressionBacterialAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTOX12
Plasmid#127457PurposeEncodes Tse2 toxin. Gent-RDepositorInserttse2 toxin
ExpressionBacterialPromoterpRhaAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1 T210D
Plasmid#68272Purposebacterial expression of Plk1 T210D mutantDepositorInsertPLK1 (PLK1 Human)
TagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationT210DPromoterT7Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his_flag
Plasmid#210404PurposeFor expression of VHH with C-terminal his and Flag tagsDepositorTypeEmpty backboneTagshis6-flagExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPAsp-hp
Plasmid#48122PurposeShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoterDepositorInsertpredicted optimal signal peptide for Bacillus megaterium protein secretion
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet
Plasmid#216386PurposeBacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)DepositorAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only