30,738 results

1. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV ie enhancer/promoter

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pBAD-pHuji

   Plasmid

   #61555

   Purpose

   Red fluorescent protein based pH indicator in bacterial expression vector

   Depositor

   Robert Campbell

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   pHuji
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pRSET-6xTR-TUBE

   Plasmid

   #110313

   Purpose

   E. coli expression and purification of 6xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   6xTR-TUBE (UBQLN1 Human)
   

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHO4d-Cas9

   Plasmid

   #67881

   Purpose

   Cas9nlsHis6 Bacterial Expression construct

   Depositor

   Michael Nonet

   Article

   Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and T7 epitope

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDawn-sfGFP

   Plasmid

   #107741

   Purpose

   Expresses superfolder GFP under the control of a blue-light transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   superfolder GFP
   

   Expression

   Bacterial

   Promoter

   pLambda

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUCP20T-E2Crimson

   Plasmid

   #78473

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   E2-Crimson
   

   Expression

   Bacterial

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET His6 Sumo TEV LIC cloning vector (2S-T)

   Plasmid

   #29711

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTA-Mob 2.0

   Plasmid

   #149662

   Purpose

   Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

   Depositor

   Bogumil Karas

   Article

   Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

   Type

   Empty backbone

   Use

   Synthetic Biology; Conjugation helper plasmid

   Expression

   Bacterial

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HMGCS2

   Plasmid

   #38984

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HMGCS2 (HMGCS2 Human)
   

   Tags

   His6-Thioredoxin-TEV

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. M13-kmR

    Plasmid

    #206861

    Purpose

    M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

    Depositor

    Irene Chen

    Article

    Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

    Insert

    Kanamycin resistance gene
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pM2s2TsR

    Plasmid

    #137923

    Purpose

    E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCold-I-Dsup

    Plasmid

    #90021

    Purpose

    To express His-tagged Dsup in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    cspA

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSIBR2.0-818-Cas12a-NT

    Plasmid

    #235168

    Purpose

    Expresses the SIBR2.0-818-Cas12a system and a NT crRNA

    Depositor

    Constantinos Patinios

    Article

    Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

    Insert

    SIBR2.0-818-Cas12a
    

    Expression

    Bacterial

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDest-566

    Plasmid

    #11517

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSFDuet1_CylLL_CylM

    Plasmid

    #208759

    Purpose

    Expresses modified cytolysin L (mCylLL) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CylLL
    CylLM
    

    Tags

    Hisx6 Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCold CL7-Cas9

    Plasmid

    #124890

    Purpose

    obtaining full Cas9 RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-2T PTP-1B

    Plasmid

    #8602

    Depositor

    Ben Neel

    Insert

    PTP-1B (PTPN1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFLxR3

    Plasmid

    #167518

    Purpose

    Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLuxB

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pH3U3-zif268 (omega)

    Plasmid

    #18046

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    zif268 binding site
    

    Expression

    Bacterial

    Mutation

    The preferred binding site for zif268 is inserted…

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHCKan-yibDp-mCherry

    Plasmid

    #127058

    Purpose

    Low phosphate inducible mCherry with N-terminal linker and His tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge