5,017 results
-
Plasmid#136237PurposeProtein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tagsDepositorInsertDmCry
TagsHis and V5ExpressionInsectAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUb RxCas13d + RxCas13d guide RNA
Plasmid#176303PurposeExpresses RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
TagsHA tagExpressionInsectPromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1-GST-hUSP7
Plasmid#63573PurposeExpression of synthetic human USP7 tagged with GSTDepositorInsertUSP7 (USP7 Human, Synthetic)
TagsGSTExpressionInsectMutationSynthetic based on human protein sequence (please…PromoterPolyhedrinAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcRP67A prM/E YFV
Plasmid#52883Purposeexpression of Yellow Fever Virus VLPs in baculovirusDepositorInsertprM/E
ExpressionInsectPromoterPolyhedrinAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL4-DBD
Plasmid#138394PurposeRMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)DepositorInsertT2A-GAL4-DBD
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
SGyA
Plasmid#160292PurposeDrives expression of Cas9 and a TdTomato fluorescent markerDepositorInsertsVas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome
TagsT2A GFPExpressionInsectAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 Flag Baf170
Plasmid#1955DepositorAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
891 pFastBac GST PTEN
Plasmid#10937DepositorAvailable SinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
ovoD1 D1B2NR (18 kb)
Plasmid#37280DepositorInsertovoD1 genomic DNA fragment
ExpressionInsectAvailable SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1
Plasmid#17743DepositorInsertPIK3R1 (PIK3R1 Human)
ExpressionInsectAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBFv-nosP-Cas9
Plasmid#138402PurposeExpresses Cas9 in the germline of DrosophilaDepositorInsertCas9-NLS
ExpressionInsectPromoterUASAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p1177-X1-8
Plasmid#38150DepositorInsertCarboxypeptidase A
TagsHHHHHHRRExpressionInsectPromoterpolyhedronAvailable SinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJC-repo1.9kb-TALE4-VP64-P10
Plasmid#104612PurposeExpresses TALE4 under the control of a 1.9kb repo enhancer elementDepositorAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.AEBP2
Plasmid#125165PurposeExpresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D CA
Plasmid#52247PurposeDrosophila expression of YFP-Ras85D constitutively active mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ61L; CA mutationPromoterGAL4Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-GCN4
Plasmid#78899PurposeExpresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell cultureDepositorInsertdCas9-10XGCN4
UseCRISPRTagsHAExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC165-20XUAS-IVS-R::PEST
Plasmid#32142DepositorInsertR::PEST
ExpressionInsectAvailable SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-BICDR1
Plasmid#111858PurposeN-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
TagsHis-tag, SNAPf-tag, TEV site, and ZZExpressionInsectMutationR250Q, Optimised for Sf9 expressionAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-wt-HaloTag
Plasmid#201551PurposeExpresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1 (partial)
TagsHaloTagExpressionInsectMutationCodon-optimized for Drosophila expressionPromoterAc5Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
57F03_LexA-GAD
Plasmid#83256PurposeExpresses LexA-GAD under the control of 57F03 enhancerDepositorInsert57F03 enhancer
ExpressionInsectAvailable SinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
Tags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PPT1
Plasmid#25205PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLfa-PUb-MCS
Plasmid#52908PurposeAedes aegypti polyubiquitin promoter followed by MCSDepositorTypeEmpty backboneExpressionInsectPromoterAe aegptyi polyubiquitinAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6
Plasmid#198408PurposeExpresses FLAG-tagged human IntS6DepositorAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(M)Cas9
Plasmid#127384PurposeExpresses Cas9 at medium levelDepositorInsertuORF(M)-Cas9
UseCRISPRExpressionInsectAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC305
Plasmid#118898Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and TwinStrep-MB…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFast-Flag-hHus1
Plasmid#222470PurposeExpresses hHus1 in insect cellsDepositorAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_HT_B_6xHis-TEV-GFP-NBR1
Plasmid#190928PurposePlasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB[nos-Cas9 PUb-ZsGreen]
Plasmid#169011PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacExpressionInsectPromoterDrosophila melanogaster nanosAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc V5 dDispatched WT HA
Plasmid#126405PurposeExpression of V5 dDispatched WT HA in Drosophila cells lines.DepositorAvailable SinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC4-3XUAS-IVS-mCD8::GFP
Plasmid#26217DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE3-VP64-P10
Plasmid#104607PurposeExpresses TALE3 under UAS controlDepositorInsertTALE3
UseTALENExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRB14
Plasmid#52522Purposeexpresses a myc-tagged version of hCas9 in DrosophilaDepositorInsertS. pyogenes cas9 with humanized codon bias
TagsmycExpressionInsectPromotertubulinAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINN-GFP
Plasmid#53541PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
TagsN-His-GFPExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCoofy51
Plasmid#122005PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage siteDepositorTypeEmpty backboneTagsTwinStrepExpressionInsectAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy60
Plasmid#122012PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage siteDepositorTypeEmpty backboneTagsHis6-eGFPExpressionInsectAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-EGFP-Actin5c
Plasmid#15312DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE4-VP64-P10
Plasmid#104608PurposeExpresses TALE4 under UAS controlDepositorInsertTALE4
UseTALENExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
dVGlut-Gal80
Plasmid#52555PurposeExpresses Gal80 under vesicular glutamate transporter promoterDepositorExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp2::PEST
Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
ExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQUASp
Plasmid#46162DepositorTypeEmpty backboneExpressionInsectAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
47 pLIB_GST
Plasmid#160272PurposeN-term 3C cleavable GST tag for insect cell expressionDepositorTypeEmpty backboneTagsGSTExpressionInsectPromoterpolyhydrinAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa
Plasmid#86452PurposeUAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFsDepositorInsertUAS-Tc'hsp_p-tGFP-SV40
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124434PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in DrosophilaDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
ExpressionInsectAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastbacHTB HA-human PP2A Cα D88N
Plasmid#132633Purposeexpresses HA-human PP2A Cα D88N in Hi5 insect cellDepositorInsertPP2A Cα D88N
ExpressionInsectAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCGMbX
Plasmid#42300DepositorTypeEmpty backboneTagsBiP signal sequence, His6, and V5-epitopeExpressionInsectPromotermetallothoneinAvailable SinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -