4,932 results

1. pBPHLWL-VP16AD

   Plasmid

   #26259

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   VP16AD-ZIP+
   

   Use

   Drosophila transgenesis

   Expression

   Bacterial and Insect

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUASp YFP Ras85D CA

   Plasmid

   #52247

   Purpose

   Drosophila expression of YFP-Ras85D constitutively active mutant

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q61L; CA mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pFastBac1 hBRM

   Plasmid

   #1956

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   BRM (SMARCA2 Human)
   

   Tags

   flag

   Expression

   Insect

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pMT GFP

   Plasmid

   #126657

   Purpose

   Inducible expression of GFP in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   GFP
   

   Expression

   Insect

   Available Since

   Dec. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc-mCh-Tub

   Plasmid

   #24288

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Alpha-Tubulin (alphaTub84B Fly)
   

   Tags

   mCherry

   Expression

   Insect

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pKL

   Plasmid

   #110741

   Purpose

   Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Polyhedrin and p10 promoters

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1 Flag Baf170

   Plasmid

   #1955

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   BAF170 (SMARCC2 Human)
   

   Tags

   flag

   Expression

   Insect

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124434

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Expression

   Insect

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIB_GST-TEV-SRC (Y530F)

   Plasmid

   #223743

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Src Kinase (SRC Human)
   

   Tags

   GST-TEV

   Expression

   Insect

   Mutation

   Y530F constitutively active mutant

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

    Plasmid

    #217512

    Purpose

    KD Recombinase dependent conditional smGdP-10XOllas cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
    

    Tags

    smGdP-10XOllas

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ac5-STABLE1-blast

    Plasmid

    #208033

    Purpose

    Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pattB-synaptobrevin-7-QFBDAD-hsp70

    Plasmid

    #46115

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]

    Plasmid

    #86449

    Purpose

    pBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)

    Depositor

    Gregor Bucher

    Insert

    3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOEM1_pCMV:hShh-pPH:vsvged

    Plasmid

    #111156

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, hShh

    Depositor

    Elly Tanaka

    Article

    Nacu et al Nature. 2016 May 19;533(7603):407-10. do

    Insert

    hShh (SHH Human)
    

    Use

    Baculoviral rescue shuttle vector

    Expression

    Insect and Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

    Plasmid

    #48295

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    His6

    Expression

    Insect

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4

    Plasmid

    #217895

    Purpose

    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4)

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCoofy64

    Plasmid

    #122016

    Purpose

    Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His6-Smt3Star and Multi3: depending on the LP2 pr…

    Expression

    Insect

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBac{3XP3::DsRed, Pactin::Ptrsps}

    Plasmid

    #86862

    Purpose

    Expresses DsRed in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Expression

    Insect

    Mutation

    S129A

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

    Plasmid

    #63169

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-FLAG"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218866

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. RhoC FLARE.sc mCer, mVenus - wt

    Plasmid

    #65071

    Purpose

    RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus

    Promoter

    CMV, p10

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pBABR IRFP-Bcd-LEXY

    Plasmid

    #182595

    Purpose

    Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Tags

    IRFP and LEXY

    Expression

    Insect

    Promoter

    maternal tubulin

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pUAST-attB-GFP-V5-His

    Plasmid

    #85621

    Purpose

    Expresses GFP and V5 in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Rauskolb et al PLoS Biol. 2011 Jun;9(6):e1000624. doi:

    Insert

    GFP
    

    Tags

    V5, His

    Expression

    Insect

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFasBac+GFP-CAMSAP3

    Plasmid

    #59038

    Purpose

    for baculovirus expression of mouse CAMSAP3 in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 (Camsap3 Mouse)
    

    Tags

    His and eGFP

    Expression

    Insect

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. OA-1053B

    Plasmid

    #184007

    Purpose

    Expresses arrays of gRNA targeting hh promoter under U6b/c promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    U6b:sgRNAhh1 - U6c:sgRNAhh2
    

    Expression

    Insect

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTK

    Plasmid

    #165841

    Purpose

    ganciclovir sensitivity marker

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    SR39TK
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTriEx-mCherry-Zdk1-TIAM1 DH/PH

    Plasmid

    #81129

    Purpose

    mammalian expression of Zdk1 tagged Tiam1 Dh/PH

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    TIAM1 DH/PH (TIAM1 Human)
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 10XSTAT92E–luciferase

    Plasmid

    #37393

    Depositor

    Norbert Perrimon

    Article

    Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

    Insert

    SOCS36E enhancer fragment (Socs36E Fly)
    

    Use

    Luciferase; Jak/stat reporter construct

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFBD-BirA_RACK1:1-317

    Plasmid

    #210889

    Purpose

    Insect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    RACK1:M1-R317 (RACK1 Human)
    

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pHACK(Gal4)-DONR(T2A-LexAGAD)

    Plasmid

    #194769

    Purpose

    To insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.

    Depositor

    Chun Han

    Article

    Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

    Insert

    T2A-LexAGAD
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Expression

    Insect

    Promoter

    Drosophila melanogaster nanos

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKDM

    Plasmid

    #110740

    Purpose

    Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. UAS-u(M)Cas9

    Plasmid

    #127384

    Purpose

    Expresses Cas9 at medium level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(M)-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBFv-UAS3

    Plasmid

    #138399

    Purpose

    10xUAS expression vector with a vermilion marker and a phiC31 attB sequence

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    UAS

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHACK-QF2

    Plasmid

    #80274

    Purpose

    QF2 HACK backbone. Contains QF2-hsp70, but no gRNAs

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFastBac1 PIK3R1

    Plasmid

    #17743

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Expression

    Insect

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pAW-CD8-Nb127D01-GFP

    Plasmid

    #171577

    Purpose

    Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    CD8-Nb127D01-GFP (CXCR2 Human)
    

    Tags

    CD8 and GFP

    Expression

    Insect

    Promoter

    fly actin5C promoter

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAct:dCas9-GCN4

    Plasmid

    #78899

    Purpose

    Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-10XGCN4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJFRC165-20XUAS-IVS-R::PEST

    Plasmid

    #32142

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    R::PEST
    

    Expression

    Insect

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pOPINNUSA

    Plasmid

    #41119

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge