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pTriEx-PA-Rac1

Plasmid

#22024

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6X His

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pBIG1c

Plasmid

#80613

Purpose

pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

Plasmid

#133560

Purpose

Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBIG1d

Plasmid

#80614

Purpose

pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

Plasmid

#55218

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pNos-PE2-attB

Plasmid

#149549

Purpose

Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Expression

Insect

Promoter

nanos (nos)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-URH49 WT

Plasmid

#110127

Purpose

Expresses Flag-tagged human URH49 (DDX39A)

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Tags

Flag

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

April 6, 2019

Availability

Academic Institutions and Nonprofits only

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pECIA14

Plasmid

#47051

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Alkaline Phosphatase (human, placental), Flag, HR…

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xMCP-2xTagRFP-T

Plasmid

#71242

Purpose

Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

NLS-2xMCP-2xTagRFP-T

Use

P element-mediated germline transformation

Tags

TagRFP-T

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-Siwi

Plasmid

#50560

Purpose

Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

Siwi (Aubergine )

Tags

6xHIS and FLAG

Expression

Insect

Promoter

OpIE2

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aaeg_774

Plasmid

#176673

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Tags

Hexa his

Expression

Insect

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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6X-STAT-synthetic luciferase

Plasmid

#37392

Depositor

Norbert Perrimon

Article

Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

Insert

six STAT consensus sites

Use

Luciferase

Expression

Insect

Promoter

Hsp70

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pJFRC12-10XUAS-IVS-myr::GFP

Plasmid

#26222

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

myr::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pAcBac1-BacMam-4x-PyOtR

Plasmid

#217364

Purpose

Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

4 copies of pyrrolysyl-tRNA mutant PyOtR

Use

Baculoviral

Expression

Insect and Mammalian

Promoter

U6

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-LexGAD-3xP3-RFP

Plasmid

#127558

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

LexGAD

Use

CRISPR

Expression

Insect

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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PRExpress

Plasmid

#122486

Purpose

A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Inserts

LacZ
poly-PRE

Expression

Insect

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1_hPMS2

Plasmid

#129425

Purpose

Expression human PMS2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human PMS2 (PMS2 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDmAct5C::Cas9-2A-Neo

Plasmid

#176679

Purpose

Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)

Use

Crispr

Expression

Insect

Mutation

Drosophila codon optimized

Promoter

D. melanogaster Act5C

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-melHis10TEV

Plasmid

#159428

Purpose

empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites

Depositor

Debra Hansen

Type

Empty backbone

Tags

melittin signal sequence (removed in vivo by sign…

Expression

Insect

Promoter

polyhedrin

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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