4,932 results

1. p{ie1-eGFP::GW,attB}

   Plasmid

   #182172

   Purpose

   fluorescent reporter gene with intron containing Gateway cloning cassette

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   eGFP
   

   Expression

   Insect

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p{Mhc-F4-678-DsRed::GW,attB}

   Plasmid

   #182171

   Purpose

   fluorescent reporter gene with intron containing Gateway cloning cassette

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   DsRed
   

   Expression

   Insect

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AT5G63930 X059_pECIA2

   Plasmid

   #115116

   Purpose

   Bait vector AT5G63930 X059_pECIA2 should be used with prey vector AT5G63930 X059_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT5G63930 (AT5G63930 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AT1G17230 X074_pECIA2

   Plasmid

   #115096

   Purpose

   Bait vector AT1G17230 X074_pECIA2 should be used with prey vector AT1G17230 X074_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G17230 (AT1G17230 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AT2G33170 X049_pECIA2

   Plasmid

   #115101

   Purpose

   Bait vector AT2G33170 X049_pECIA2 should be used with prey vector AT2G33170 X049_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT2G33170 (AT2G33170 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AT1G74360 X038_pECIA2

   Plasmid

   #115080

   Purpose

   Bait vector AT1G74360 X038_pECIA2 should be used with prey vector AT1G74360 X038_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G74360 (AT1G74360 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDrBB2

   Plasmid

   #18946

   Depositor

   Michele Calos

   Article

   Groth et al Genetics. 2004 Apr . 166(4):1775-82.

   Insert

   hygromycin gene driven by the copia promoter, phiC31 attB
   

   Expression

   Insect

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. AT5G63930 X059_pECIA14

   Plasmid

   #114916

   Purpose

   Prey vector AT5G63930 X059_pECIA14 should be used with bait vector AT5G63930 X059_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT5G63930 (AT5G63930 Mustard Weed)
   

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AT1G17230 X074_pECIA14

   Plasmid

   #114896

   Purpose

   Prey vector AT1G17230 X074_pECIA14 should be used with bait vector AT1G17230 X074_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G17230 (AT1G17230 Mustard Weed)
   

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AT2G33170 X049_pECIA14

    Plasmid

    #114901

    Purpose

    Prey vector AT2G33170 X049_pECIA14 should be used with bait vector AT2G33170 X049_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT2G33170 (AT2G33170 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AT1G74360 X038_pECIA14

    Plasmid

    #114880

    Purpose

    Prey vector AT1G74360 X038_pECIA14 should be used with bait vector AT1G74360 X038_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G74360 (AT1G74360 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUAST>stop>mCD8-GFP

    Plasmid

    #24385

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    mCD8-GFP
    

    Expression

    Insect

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

    Plasmid

    #169182

    Purpose

    Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    His6-3xFlag

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

    Plasmid

    #32139

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    B3RT> STOP STOP B3RT>
    

    Expression

    Insect

    Available Since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINTF

    Plasmid

    #41114

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-HemmarG

    Plasmid

    #31221

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Insert

    CD4-tdGFP (CD4 Fly, Human, Aequorea victoria)
    

    Tags

    CD4 and EGFP/GFP

    Expression

    Insect

    Mutation

    Fusion of signal peptide of Drosophila Akh gene, …

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPac Sp1

    Plasmid

    #12095

    Depositor

    Robert Tjian

    Article

    Courey et al Cell. 1988 Dec 2. 55(5):887-98.

    Insert

    Sp1 (SP1 Human)
    

    Expression

    Insect

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

18. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUAS-Myc-BirA-G3-ER

    Plasmid

    #173815

    Purpose

    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

    Depositor

    Norbert Perrimon

    Article

    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

    Insert

    Myc-BirA-G3-ER
    

    Tags

    Myc

    Expression

    Insect

    Promoter

    UAS

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJFRC-20XUAS-IVS-PhiC31

    Plasmid

    #133565

    Purpose

    Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEM-3XP3.RFP-tan

    Plasmid

    #83474

    Purpose

    RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

    Depositor

    Patricia Wittkopp

    Article

    Lamb et al Fly (Austin). 2016 Aug 5:1-12.

    Insert

    3XP3.RFP-tan
    

    Use

    CRISPR

    Expression

    Bacterial and Insect

    Promoter

    3XP3 eye-expression Promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCRISPaint-T2A-Gal80-3xP3-RFP

    Plasmid

    #127562

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal80
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBiex-1 Myosin VI HMM_SNAP

    Plasmid

    #82716

    Purpose

    Expresses Myosin VI HMM with a C-terminal SNAP tag.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

    Insert

    Myosin VI
    

    Tags

    6xHis Tag, FLAG purification tag, and SNAP tag

    Expression

    Bacterial and Insect

    Promoter

    T7 Promoter

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 438-GST

    Plasmid

    #97092

    Purpose

    GST-tev-yORF

    Depositor

    Chris Jeans

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    GST tag and TEV cleavage site

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBPGAL80Uw-4

    Plasmid

    #26235

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRISPaint-T2A-FLPo-3xP3-RFP

    Plasmid

    #127561

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    FLPo
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQUASp-RedStinger (nlsRFP)

    Plasmid

    #46165

    Purpose

    Expression of RedStinger in insect

    Depositor

    Christopher Potter

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOCC7

    Plasmid

    #118876

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pQUAST-mCD8-GFP

    Plasmid

    #24351

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    CD8-GFP-SV40
    

    Expression

    Insect

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUb FLAG-Human IntS6L

    Plasmid

    #198409

    Purpose

    Expresses FLAG-tagged human IntS6L

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator complex subunit 6 like (INTS6L Human)
    

    Tags

    3x FLAG

    Expression

    Insect

    Promoter

    Ubi-p63e

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

    Plasmid

    #217893

    Purpose

    Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMT-Diaphanous-GFP

    Plasmid

    #24287

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Diaphanous (dia Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

33. p(bhsp68-Cas9)

    Plasmid

    #65959

    Purpose

    Tribolium basal heat shock promoter driving Cas9

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    nuclear localization sequence

    Expression

    Insect

    Promoter

    bhsp

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

    Plasmid

    #32145

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    loxP> STOP STOP loxP>
    

    Expression

    Insect

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMT-HA-STX1

    Plasmid

    #89310

    Purpose

    Expresses HA tagged syntaxin 1 in Drosophila cells

    Depositor

    Andrew Peden

    Insert

    STX1 (Syx1A Fly)
    

    Tags

    HA tag

    Expression

    Insect

    Promoter

    P-MT (Metallothionein)

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AE-Polyubiquitin-GCaMP6s

    Plasmid

    #106868

    Purpose

    Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

    Depositor

    Omar Akbari

    Article

    Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

    Insert

    AE-Polyubiquitin-GCAaMP6s
    

    Expression

    Insect

    Promoter

    Ae. aegypti polyubiquitin (PUb)

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pw35Gal4

    Plasmid

    #25901

    Depositor

    Craig Montell

    Article

    Moon et al Curr Biol. 2009 Oct 13. 19(19):1623-7.

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

39. p(U6b-BsaI-gRNA)

    Plasmid

    #65956

    Purpose

    Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    U6

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pE3n

    Plasmid

    #17457

    Purpose

    modified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    6xMyc

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge