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pRmHA3-CH2F2-CG9866

Plasmid

#52286

Purpose

Expresses Drosophila Ataxin-7

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Use

Tags

2xHA 2xFLAG

Expression

Insect

Mutation

Promoter

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_1

Plasmid

#127554

Purpose

Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAct-PE2-HygroR

Plasmid

#149552

Purpose

Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

Tags

Expression

Insect

Mutation

Promoter

Act5c

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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UAS-myr::4xSNAPf

Plasmid

#87637

Purpose

For creating drosophila transgenics expressing UAS-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pUASattB-PKA-SPARK

Plasmid

#106922

Purpose

Express PKA-SPARK, an activity reporter of protein kinase A in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

PKA-SPARK

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(E17K)

Plasmid

#86563

Purpose

Expresses human full-length Akt1(E17K) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

E17K mutation in the PH domain

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pIEx4-Dio-GFP

Plasmid

#198278

Purpose

Cre reporter for insect cells; double-floxxed-EGFP

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pIEx4-Dio-GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFC31

Plasmid

#165845

Purpose

_C31 integrase CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 integrase

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBac{3XP3::EGFP, Pactin::Ptrsps}

Plasmid

#86861

Purpose

Expresses EGFP in eyes and ubiquitous expression of P-transposase

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

pACNNTNPII-S129A

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-QF

Plasmid

#24338

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

QF

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Use

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_2

Plasmid

#127555

Purpose

Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD8

Plasmid

#140619

Purpose

Expression of one of several LbCas12a crRNAs in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6:3

Available since

Oct. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUWR-CadTS-C

Plasmid

#58326

Purpose

Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

E-cadherin tension sensor control

Use

Tags

Expression

Insect

Mutation

Promoter

poly-ubiquitin

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCRISPaint-T2A-sfGFP-3xP3-RFP

Plasmid

#127557

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sfGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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AlphaC

Plasmid

#124525

Purpose

pColE1_AlphaC

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Gal4DBD2xHighMag1

Plasmid

#182023

Purpose

Donor plasmid for conversion of Gal4 line into ShineGal4 drivers

Depositor

Jean-Paul Vincent

Article

di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

Insert

Gal4DBD2xHighMag1

Use

Donor plasmid

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

Plasmid

#169182

Purpose

Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

His6-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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p2Bac.XPC

Plasmid

#39204

Depositor

Aziz Sancar

Article

Reardon et al J Biol Chem. 1996 Aug 9;271(32):19451-6.

Insert

XPC (XPC Human)

Use

Tags

Expression

Insect

Mutation

Q475K polymorphism

Promoter

Pp10

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pPac Sp1

Plasmid

#12095

Depositor

Robert Tjian

Article

Courey et al Cell. 1988 Dec 2. 55(5):887-98.

Insert

Sp1 (SP1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Vector 1174D

Plasmid

#200012

Purpose

Fluorescent based sex separator in Aedes aegypti (SEPARATOR)

Depositor

Omar Akbari

Article

Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.

Insert

Engineered dobulesex splicing module

Use

Synthetic Biology

Tags

Expression

Insect

Mutation

Engineered dobulesex splicing module

Promoter

Hr5IE1

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQUASp

Plasmid

#46162

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pACUH-sfcherry2(1-10)

Plasmid

#172061

Purpose

UAS vector to express sfcherry2(1-10) in Drosophila

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

sfcherry2(1-10)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p3X-UASTattB

Plasmid

#189859

Purpose

Fly cloning and transformation

Depositor

Jung Hwan Kim

Article

Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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TH0901-pOCC177-FUS-wt_opt(Nhe)

Plasmid

#221885

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-LIC_DDB1:1-1140_delta:396-689

Plasmid

#210897

Purpose

Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)

Use

Tags

Expression

Insect

Mutation

loop deletion amino acids I396 to D689

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRB14

Plasmid

#52522

Purpose

expresses a myc-tagged version of hCas9 in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

S. pyogenes cas9 with humanized codon bias

Use

Tags

myc

Expression

Insect

Mutation

Promoter

tubulin

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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ptubP-QS

Plasmid

#24352

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

QS

Use

Tags

white gene

Expression

Insect

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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pBIG1b_10xHisTEVATG5_ATG12

Plasmid

#190861

Purpose

Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG12 (ATG12 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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