5,046 results

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pGal80,y+

Plasmid

#26851

Depositor

Christopher Potter

Insert

Gal80 (GAL80 Budding Yeast)

Tags

Yellow Gene (Drosophila)

Expression

Insect

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST8_FLAGHA_FUS_HIS

Plasmid

#26375

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Tags

FLAG/HA and His

Expression

Insect

Mutation

delta 527 (STOP codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pMT-Diaphanous-GFP

Plasmid

#24287

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Diaphanous (dia Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pQUAST>stop>shibire (ts1)

Plasmid

#24363

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

Shibire (ts1) (shi Fly)

Expression

Insect

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Tipin

Plasmid

#22891

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pIE2-AD

Plasmid

#21603

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

NF-kappa-B p65 subunit (Rela Mouse)

Use

Gateway Cloning

Tags

Flag, Gateway destination cassette, and NLS

Expression

Insect

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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CG4845/pMT/V5-HisB

Plasmid

#17589

Depositor

Denise Montell

Insert

CG4845 (psidin Fly)

Tags

6x His and V5

Expression

Insect

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
Jag1ICD-HA pBacPAK8-3

Plasmid

#17331

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Jagged1 (Jag1 Rat)

Tags

HA

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac FPC

Plasmid

#1927

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

Polycomb (Pc Fly)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

Plasmid

#214138

Purpose

Insect cell expression of human 20S CP alpha-subunits

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)
PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

Plasmid

#213025

Purpose

Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

Depositor

Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

LINE-1 ORF2p (1-1275)

Tags

3C-3xFlag (ORF2p)

Expression

Insect

Promoter

Polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSSRB070_pAC8-hsCRBN

Plasmid

#218789

Purpose

Insect cell expression vector for FLAG-TEV-Spy-hsCRBN

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

Protein cereblon (CRBN Human)

Tags

Flag-TEV-Spy

Expression

Insect

Promoter

polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-split-intein-Gal4[1-20]-N-int

Plasmid

#199201

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[1-20]-N-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pADC10-SETXhel

Plasmid

#193056

Purpose

Baculovirus expression of human Senataxin helicase domain for protein production

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

Senataxin (SETX Human)

Tags

Flag

Expression

Insect and Mammalian

Mutation

codon optimized for insect cell expression

Promoter

hybrid p10 (insect) and CMV (human) expression

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC BAF155/SMARCC1-His

Plasmid

#177863

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCC1 (SMARCC1 Human)

Tags

His and His tag at C-terminus with GGGGS linker

Expression

Insect

Promoter

polyhedrin

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDmAct5C::Cas9-2A-Neo

Plasmid

#176679

Purpose

Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)

Use

Crispr

Expression

Insect

Mutation

Drosophila codon optimized

Promoter

D. melanogaster Act5C

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTF023_His_hsDDA1

Plasmid

#174480

Purpose

Expresses DDA1 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DDA1 (DDA1 Human)

Tags

His-TEV

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3x127D01-DsRed

Plasmid

#171578

Purpose

For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

Plasmid

#169077

Purpose

Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG7 (ATG7 Human)
ATG10 (ATG10 Human)
ATG12 (ATG12 Human)
ATG16 (ATG16L1 Human)

Tags

GFP tag, GGlinker, TEV cleavage site. StrepII and…

Expression

Bacterial and Insect

Promoter

Polyhedrin promoter

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

Plasmid

#162384

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-u(M)Cas9

Plasmid

#127384

Purpose

Expresses Cas9 at medium level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(M)-Cas9

Use

CRISPR

Expression

Insect

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

Plasmid

#162164

Purpose

Selectable homology donor template for Aag2 cell AGO1

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

RFP
3xFLAG

Use

CRISPR and Cre/Lox; Homology donor template

Tags

AGO1 homology and loxP

Expression

Insect

Promoter

Ae. aegypti PUb and endogenous AGO1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Tags

Hexa his

Expression

Insect

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDest-635

Plasmid

#159582

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDest-636

Plasmid

#159574

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Type

Empty backbone

Tags

His6-MBP

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Brp-T2A-QF2w-Hsp70-3XP3-dsRed

Plasmid

#141094

Purpose

Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

bruchpilot

Use

CRISPR

Expression

Insect

Mutation

coding sequence downstream of CRISPR cut site, sy…

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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IMPT-10316:GABR1

Plasmid

#157850

Purpose

HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABR1 (GABBR1 Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAct-GW-HygroR

Plasmid

#149610

Purpose

Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

Gateway cassette (ccdb + Chlor.R.)

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.EED

Plasmid

#125174

Purpose

Expresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Tags

His-TEV

Expression

Insect

Promoter

Polyhedrin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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