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5,046 results

Showing: 751 - 780 of 5046 results

  6. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

    Plasmid
    #169182
    Purpose
    Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    His6-3xFlag
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169182-plasmid-map-sequence-id-333999

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  9. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  11. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Article
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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  12. pDest-795

    Plasmid
    #161881
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell system
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Tags
    MBP
    Expression
    Insect
    Available Since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    161881-plasmid-map-sequence-id-322112

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  26. pKM50

    Plasmid
    #123656
    Purpose
    Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells
    Depositor
    Kevin Maringer
    Article
    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0
    Insert
    Aedes aegypti UbL40 promoter
    Use
    Luciferase
    Expression
    Insect
    Promoter
    UbL40 promoter
    Available Since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    123656-plasmid-map-sequence-id-243712

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  28. pOCC28

    Plasmid
    #118873
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118873-plasmid-map-sequence-id-232344

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  29. pOCC72

    Plasmid
    #118866
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mGFP, cleavable…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118866-plasmid-map-sequence-id-232323

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  30. pOCC102

    Plasmid
    #118883
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118883-plasmid-map-sequence-id-232315

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Showing: 751 - 780 of 5046 results