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5,017 results

Showing: 1251 - 1300 of 5017 results

  5. DPYSL4 (DPYSL4A-c003)

    Plasmid
    #110263
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPYSL4 (DPYSL4 Human)
    Tags
    His6-ZBasic-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110263-plasmid-map-sequence-id-212341

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  8. pAc5.1B-lambdaN-HA-DmARC1_N

    Plasmid
    #146978
    Purpose
    Insect Expression of DmARC1
    Depositor
    Elisa Izaurralde
    Insert
    DmARC1 (Arc1 Fly)
    Expression
    Insect
    Mutation
    5 silent mutations and one non silent mutation G3…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146978-plasmid-map-sequence-id-389936

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  13. p2Bac.Myc.p44

    Plasmid
    #39220
    Depositor
    Aziz Sancar
    Insert
    p44 (GTF2H2 )
    Tags
    Myc
    Expression
    Insect
    Promoter
    Pp10
    Available Since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  16. pOPINO_F2

    Plasmid
    #171926
    Purpose
    Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_F2
    Tags
    His6
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171926-plasmid-map-sequence-id-338638

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  20. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do
    Insert
    Full-length engineered CD64, derived from human CD64
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    109191-plasmid-map-sequence-id-233794

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  24. pOCC240

    Plasmid
    #118895
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and mCherry, clea…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118895-plasmid-map-sequence-id-232366

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  26. pUASp YFP Ras64B DN

    Plasmid
    #52243
    Purpose
    Drosophila expression of YFP-Ras64B dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Ras64B (Ras64B Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    S19N; DN mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pUASp YFP Ras64B CA

    Plasmid
    #52244
    Purpose
    Drosophila expression of YFP-Ras64B constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Ras64B (Ras64B Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q63L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pUASp YFP Ras64B WT

    Plasmid
    #52245
    Purpose
    Drosophila expression of YFP-Ras64B
    Depositor
    Matthew Scott
    Insert
    Ras64B (Ras64B Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Promoter
    GAL4
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pFastBacDual with LFn + FlaAAA

    Plasmid
    #84867
    Purpose
    Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    FlaAAA
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    Polyhedrin
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84867-plasmid-map-sequence-id-162897

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  33. pMT[Hygro]_GBP-3xFlag-DEST

    Plasmid
    #157813
    Purpose
    A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection
    Depositor
    Zoltan Lipinszki
    Article
    Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:
    Insert
    GFP-binding protein
    Use
    For drosophila cells
    Tags
    3xFlag
    Expression
    Insect
    Promoter
    metallothionein
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    157813-plasmid-map-sequence-id-311081

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  50. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS3 (HAUS3 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS2 (HAUS2 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS6 (HAUS6 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS7 (HAUS7 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS4 (HAUS4 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS5 (HAUS5 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS8 (HAUS8 Human, codon-optimized form for expression in Trichoplusia ni cells)
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

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Showing: 1251 - 1300 of 5017 results