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5,038 results

Showing: 1251 - 1300 of 5038 results

  1. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid
    #169185
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    His6-3xFlag (nsp12 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169185-plasmid-map-sequence-id-334217

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  2. CDK12

    Plasmid
    #73258
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CDK12 (CDK12 Human)
    Tags
    His-6-TEV
    Expression
    Insect
    Mutation
    pfam00069: Pkinase 727-1020
    Promoter
    Polyhedrin
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pOCC267

    Plasmid
    #118887
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; HALO tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118887-plasmid-map-sequence-id-232249

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  16. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid
    #218865
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Article
    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.
    Insert
    RSET-jGCaMP8s
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    S26M F286Y Q315H
    Promoter
    hsp70
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218865-plasmid-map-sequence-id-434255

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  21. MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

    Plasmid
    #216385
    Purpose
    Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)
    Depositor
    William Weis
    Article
    Bruguera et al bioRxiv 2024.02.03.578714
    Insert
    Norrin (NDP Human)
    Use
    Baculovirus
    Tags
    GP64 signal sequence-MBP-3C and Rho1D4
    Expression
    Insect
    Mutation
    C93A/C95A/C131A
    Promoter
    Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216385-plasmid-map-sequence-id-433265

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  26. pUASp YFP Ras85D DN

    Plasmid
    #52246
    Purpose
    Drosophila expression of YFP-Ras85D dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Ras85D (Ras85D Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    S17N; DN mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do
    Insert
    Full-length engineered CD64, derived from human CD64
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    109191-plasmid-map-sequence-id-233794

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  32. pOPINO_F2

    Plasmid
    #171926
    Purpose
    Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_F2
    Tags
    His6
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171926-plasmid-map-sequence-id-338638

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  34. pOCC240

    Plasmid
    #118895
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and mCherry, clea…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118895-plasmid-map-sequence-id-232366

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  37. DPYSL4 (DPYSL4A-c003)

    Plasmid
    #110263
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPYSL4 (DPYSL4 Human)
    Tags
    His6-ZBasic-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110263-plasmid-map-sequence-id-212341

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  38. pFastBacDual with LFn + FlaAAA

    Plasmid
    #84867
    Purpose
    Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    FlaAAA
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    Polyhedrin
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84867-plasmid-map-sequence-id-162897

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  39. p2Bac.Myc.p44

    Plasmid
    #39220
    Depositor
    Aziz Sancar
    Insert
    p44 (GTF2H2 )
    Tags
    Myc
    Expression
    Insect
    Promoter
    Pp10
    Available Since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  42. pAc5.1B-lambdaN-HA-DmARC1_N

    Plasmid
    #146978
    Purpose
    Insect Expression of DmARC1
    Depositor
    Elisa Izaurralde
    Insert
    DmARC1 (Arc1 Fly)
    Expression
    Insect
    Mutation
    5 silent mutations and one non silent mutation G3…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146978-plasmid-map-sequence-id-389936

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Showing: 1251 - 1300 of 5038 results