4,958 results
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Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
TagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-QF#7
Plasmid#46125DepositorInsertQF#7
ExpressionInsectAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-Cyc
Plasmid#33156DepositorAvailable SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
Or1a-Gal4
Plasmid#63019PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or1a promoter
TagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF05
Plasmid#52558Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF05
ExpressionInsectMutationchanged from GCaMP3: D397N/G398A/N399DAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-LexAQF
Plasmid#61310PurposeExpresses chimeric transactivator LexA_DBD:QF2_AD in DrosophilaDepositorInsertLexA_DBD::QF2_AD
ExpressionInsectAvailable SinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-Act5c
Plasmid#130278PurposeDrosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.DepositorAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Orp1
Plasmid#65001PurposeExpression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoG-lexA
Plasmid#138403PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertTEVcs(ENLYFQG)-LexAp65
ExpressionInsectAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC170-3XUAS-IVS-Cre::PEST
Plasmid#32144DepositorInsertCre::PEST
ExpressionInsectAvailable SinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
OA-1045C
Plasmid#125005PurposeExpresses gRNAs targeting hid, eve, and winglessDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-mScarlet-I
Plasmid#138391PurposeRMCE vector for generating C-terminal mScarlet-I fusionDepositorInsertmScarlet-I
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)
Plasmid#169180PurposeBaculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cellsDepositorInsertnsp7-His6-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
TagsHis6 (internal tag linking nsp7 and nsp8): nsp7-H…ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-p65AD::CaM
Plasmid#64717Purposegenerating transgenic TRIC fliesDepositorInsertp65AD::CaM
ExpressionInsectAvailable SinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(chimpanzee)
Plasmid#79032PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(chimpanzee)
TagsOneSTrEP-FLAGExpressionInsectPromoterpolyhedrinAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOET3-N-TEV-Twin-Strep
Plasmid#203323PurposeGenerate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
IL2 F42A pAcGP67a
Plasmid#41807DepositorInsertIL2 (IL2 Human)
Tags6x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable SinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC8_GWs-Lox (VE5589)
Plasmid#163769PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.DepositorInsertno insert
Tagsno tagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCoofy63
Plasmid#122015PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-beta-actin
Plasmid#178076PurposeInsect cell expression of beta-actinDepositorInsertbeta-actin (ACTB Human)
ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)
Plasmid#159577PurposeBaculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)DepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)
Plasmid#159578PurposeBaculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF (1541-2818)DepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
T6-pAC5.1 mEGFP-EcoRV
Plasmid#206489PurposeSchneider cell imagingDepositorTypeEmpty backboneTagsmEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTSTEP-SNAPf
Plasmid#72335PurposeTissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-SNAPf
UseCRISPRExpressionInsectAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pS3aG
Plasmid#31171PurposeA Drosophila GFP reporter transgene vector for site-specific integrationDepositorTypeEmpty backboneExpressionInsectAvailable SinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJC-TALE-GFP
Plasmid#46148DepositorTypeEmpty backboneTagsGFPExpressionInsectPromoterhsp70Available SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCaSper4 cyto roGFP2-Orp1
Plasmid#64998PurposeExpression of cytosolic roGFP2-Orp1 under the alphaTub84B promoterDepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC175
Plasmid#118890Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease; mGFP, cleav…ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC167
Plasmid#118909Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C proteaseDepositorInsertNcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Shp2)
Plasmid#177899PurposeBaculo expression of His10 tag fused to human Shp2DepositorAvailable SinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXb3
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A human LRP6 E1E2 (20–629)
Plasmid#132627Purposeexpresses human LRP6 E1E2 (20–629) in Hi5 insect cellDepositorInsertLRP6 (20–629)
ExpressionInsectAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_105_Venus_157
Plasmid#39819DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac mph1 flag
Plasmid#1985DepositorAvailable SinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::3xHalo7
Plasmid#87649PurposeFor creating drosophila transgenics expressing UAS-Syt::3xHalo7DepositorInsertSyt::3xHalo7
ExpressionInsectMutationS265G in Syt (please see depositor's commen…Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS
Plasmid#63170PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporterDepositorInsert"spaghetti monster GFP-OLLAS"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv-R896K::NG (kkv Fly)
TagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJFRC173-20XUAS-IVS-Dre::PEST
Plasmid#32146PurposeExpresses Dre recombinase under control of 20XUAS sites in DrosophilaDepositorInsertDre::PEST
ExpressionInsectPromoterHsp70Available SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPTQF#7-hsp70
Plasmid#46136DepositorInsertQF#7
ExpressionInsectAvailable SinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS Jaws mCherry_tr
Plasmid#111549PurposeOptogenetics in Drosophila: UAS-induced expression of Jaws mCherryDepositorInsertJaws_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)
Plasmid#126024Purposeexpress RFP-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
TagsRFPExpressionInsectMutationWTPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pattB-DSCP-QF-sv40
Plasmid#24369DepositorInsertQF
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_105_Venus_195
Plasmid#39821DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
RBBP4
Plasmid#25350PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertRBBP4-13 (RBBP4 Human)
UseBaculovirus expressionTagsHisExpressionInsectPromoterpolyhedrinAvailable SinceAug. 19, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAFMW-Nbr-D435A/E437A
Plasmid#40829PurposeExpresses Flag, Myc tagged mutant Nbr in Drosophila cellsDepositorInsertNbr D435A/E437A (Nbr Fly)
Tags3xFLAG and 6xMycExpressionInsectMutationD435A/E437A, part of the DEDD motif; mutations p…PromoterActin5CAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
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3xTRE DREaMR 3p3GFP
Plasmid#182205Purpose3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-GFP
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
DmU6 3p3 GFP LgRNA
Plasmid#182207PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in DrosophilaDepositorInsert3XP3-GFP
UseCRISPRExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only