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5,038 results

Showing: 1501 - 1550 of 5038 results

  2. Ths pUAST-HA

    Plasmid
    #69769
    Purpose
    Thisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    Klingseisen et al Development. 2009 Jul;136(14):2393-402.
    Insert
    Thisbe (FGF8like-1)
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    Encodes amino acids 1-739 of Thisbe (NP_610701.2)…
    Promoter
    hsp70 promoter
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. Pyr pUAST-HA

    Plasmid
    #69768
    Purpose
    Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    Klingseisen et al Development. 2009 Jul;136(14):2393-402.
    Insert
    Pyramus (FGF8like-2) (pyr Fly)
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    241T amino acid insertion compared to reference s…
    Promoter
    hsp70 promoter
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pOPINH

    Plasmid
    #53537
    Purpose
    pTriex-based vector with signal sequence and N-his tag for protein expression
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53537-plasmid-map-sequence-id-83418

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  6. pUASp YFP Rac1 CA

    Plasmid
    #52235
    Purpose
    Drosophila expression of YFP-Rac1 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. 892 pFastBacHTb PTEN

    Plasmid
    #10938
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    His
    Expression
    Insect
    Available Since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. pGEM-WingGFP-tan

    Plasmid
    #83473
    Purpose
    EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
    Depositor
    Patricia Wittkopp
    Article
    Lamb et al Fly (Austin). 2016 Aug 5:1-12.
    Insert
    WingGFP-tan
    Use
    CRISPR
    Expression
    Bacterial and Insect
    Promoter
    Hsp70 and enhancer from D. melanogaster yellow ge…
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83473-plasmid-map-sequence-id-159157

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  20. pExpreS2-1-CR-PA-10H-SIII

    Plasmid
    #175447
    Purpose
    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    BiP Secretion Peptide and Thrombin-Protein A-10Hi…
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175447-plasmid-map-sequence-id-358139

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  23. pOCC121

    Plasmid
    #118893
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118893-plasmid-map-sequence-id-232270

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  24. pOPINEneo

    Plasmid
    #53536
    Purpose
    pTriex-based expression vector which adds a C-his8 tag to the inserted gene
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    53536-plasmid-map-sequence-id-83415

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  25. pLib6.4B

    Plasmid
    #176665
    Purpose
    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Expression
    Insect
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176665-plasmid-map-sequence-id-348636

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  37. pOCC70

    Plasmid
    #118878
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    Tags
    mGFP, cleavable with TEV protease; HIS6, cleavabl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118878-plasmid-map-sequence-id-232355

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  46. pAB-LIC C

    Plasmid
    #62288
    Purpose
    Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    8XHis tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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Showing: 1501 - 1550 of 5038 results