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5,017 results

Showing: 1501 - 1550 of 5017 results

  4. pUASp YFP Rac1 CA

    Plasmid
    #52235
    Purpose
    Drosophila expression of YFP-Rac1 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pOPINH

    Plasmid
    #53537
    Purpose
    pTriex-based vector with signal sequence and N-his tag for protein expression
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53537-plasmid-map-sequence-id-83418

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  8. 892 pFastBacHTb PTEN

    Plasmid
    #10938
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    His
    Expression
    Insect
    Available Since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  9. pOCC121

    Plasmid
    #118893
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118893-plasmid-map-sequence-id-232270

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  11. pOPINEneo

    Plasmid
    #53536
    Purpose
    pTriex-based expression vector which adds a C-his8 tag to the inserted gene
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    53536-plasmid-map-sequence-id-83415

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  12. pGEM-WingGFP-tan

    Plasmid
    #83473
    Purpose
    EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
    Depositor
    Patricia Wittkopp
    Article
    Lamb et al Fly (Austin). 2016 Aug 5:1-12.
    Insert
    WingGFP-tan
    Use
    CRISPR
    Expression
    Bacterial and Insect
    Promoter
    Hsp70 and enhancer from D. melanogaster yellow ge…
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83473-plasmid-map-sequence-id-159157

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  17. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid
    #172918
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7c-T2A-tdTomato
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Promoter
    hsp70
    Available Since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172918-plasmid-map-sequence-id-343805

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  24. pOCC70

    Plasmid
    #118878
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    Tags
    mGFP, cleavable with TEV protease; HIS6, cleavabl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118878-plasmid-map-sequence-id-232355

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  29. 1161F

    Plasmid
    #183138
    Purpose
    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.
    Depositor
    Omar Akbari
    Article
    Kandul et al GEN Biotechnology (2022) 1:4, 372-385
    Insert
    U6.3-gRNAs[sxl, bTub]
    Use
    CRISPR
    Expression
    Insect
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183138-plasmid-map-sequence-id-373781

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  39. pAB-LIC C

    Plasmid
    #62288
    Purpose
    Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    8XHis tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. His-ZZ-TEV-BICDN(1-400)-GFP

    Plasmid
    #111862
    Purpose
    C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.
    Depositor
    Andrew Carter
    Article
    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do
    Insert
    BICDN(1-400)-GFP (Bicd2 Mouse)
    Tags
    8xHis tag, GFP, and ZZ tag
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111862-plasmid-map-sequence-id-215382

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  44. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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  46. R-Q111R-N122D

    Plasmid
    #167178
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-R-Q111R-N122D
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed R at 111 to Q and changed D at 122 to N
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167178-plasmid-map-sequence-id-334483

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Showing: 1501 - 1550 of 5017 results