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psiCHECK2-let-7 4x

Plasmid

#20930

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Mar 4. ():.

Insert

4x let-7 target sites

Use

Luciferase

Tags

Expression

Insect and Mammalian

Mutation

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_175_Venus_173

Plasmid

#39830

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pNos-PE2-attB

Plasmid

#149549

Purpose

Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

nanos (nos)

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-LexA

Plasmid

#26266

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

LexA

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EIF3B:127-814

Plasmid

#210909

Purpose

Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF3B:D127-E814 (EIF3B Human)

Use

Tags

MHHHHHHSSGRENLYFQGMQ

Expression

Insect

Mutation

Y760C sequence conflict

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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His-ZZ-TEV-SNAPf-BICD2N(1-400)

Plasmid

#111857

Purpose

N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICD2N(1-400)

Use

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-blast

Plasmid

#208034

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

Depositor

James Sutherland, Rosa Barrio

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCaryP

Plasmid

#18945

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 attP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pACU

Plasmid

#58373

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Chun Han, Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 May 23. (

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Rab18

Plasmid

#31740

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab18 (Rab18 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pQUAST-FLPo

Plasmid

#24357

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

FLPo

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 12, 2010

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 Exons 1-3

Plasmid

#91799

Purpose

Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

Laccase2 (stw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>GAL80

Plasmid

#104874

Purpose

HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.

Depositor

Christopher Potter

Insert

T2A-GAL80

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKSB-611U6-sgRNA3 AGAG AACA

Plasmid

#173673

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHRW-CRY2-VHH(GFP)

Plasmid

#126022

Purpose

express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHR-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMT-IMD-V5-His

Plasmid

#63748

Purpose

Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

Depositor

Cyril Gueydan

Insert

immune deficiency (imd Fly)

Use

Tags

V5-6XHis

Expression

Insect

Mutation

*see comment below

Promoter

pMT

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBac{Mhc-453-GFP,attB,w+}

Plasmid

#182199

Purpose

EGFP driven by 453bp enhancer of Mhc gene

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Agam_695

Plasmid

#176668

Purpose

Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pF3BGX-TangoVL-lexA

Plasmid

#138406

Purpose

RMCE vector for generating C-terminal Tango fusion

Depositor

Shu Kondo

Article

Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

Insert

V2 tail-TEVcs(ENLYFQL)-LexAp65

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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RAT

Plasmid

#187416

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Use

Tags

Expression

Insect

Mutation

Promoter

PH and p10

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pQUAST-mtdT-3XHA

Plasmid

#24354

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

mtDT-3XHA

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pIHEU-MCS

Plasmid

#58375

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Chun Han

Article

Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pUAST cyto roGFP2-Orp1

Plasmid

#64996

Purpose

for expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Use

Tags

roGFP2

Expression

Insect

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-SNAP-Csk)

Plasmid

#177895

Purpose

Baculo expression of His10 tag fused to SNAPtag and human Csk

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Csk (CSK Human)

Use

Tags

10xHis-TEV-TVMV-SNAP tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-7xHalo7::CAAX

Plasmid

#87647

Purpose

For creating drosophila transgenics expressing UAS-7xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

7xHalo7::CAAX

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_641-1574_AH

Plasmid

#148920

Purpose

Insect Expression of DmGIGYF_641-1574

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_641-1574 (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_1-640_AH

Plasmid

#148919

Purpose

Insect Expression of DmGIGYF_1-640

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_1-640 (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rab6

Plasmid

#31734

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab6 (Rab6 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
His-ZZ-TEV-SNAPf-DYNC2H1

Plasmid

#132524

Purpose

Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNC2H1 (DYNC2H1 Human)

Use

Tags

8x His, Linker-TEV-linker-TEV, Precission cleavag…

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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