5,046 results
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Plasmid#145920PurposeInsect Expression of DmFMR1DepositorInsertDmFMR1 (Fmr1 Fly)
ExpressionInsectMutationTwo amino acid mutations (T214I and N501S) and th…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-TuSC
Plasmid#178079PurposeInsect cell expression of gamma-TuSCDepositorTagsTEV-His6ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-GCP3
Plasmid#178069PurposeInsect cell expression of GCP3DepositorInsertGCP3 (TUBGCP3 Human)
ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-beta-actin
Plasmid#178076PurposeInsect cell expression of beta-actinDepositorInsertbeta-actin (ACTB Human)
ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_ybbR_Tau
Plasmid#159717PurposeExpresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tagDepositorInsertMicrotubule-associated protein tau (MAPT Human)
TagsZZ affinity tag, ybbR tagExpressionInsectAvailable SinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Consensus
Plasmid#176678PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACUH-sfcherry2(1-10)
Plasmid#172061PurposeUAS vector to express sfcherry2(1-10) in DrosophilaDepositorInsertsfcherry2(1-10)
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
ppBAalbNixE1E2 PUb-GFP
Plasmid#173667Purposetransgenesis vector for Aedes albopictus ectopic expression of the Nix geneDepositorInsertsNix masculinisation gene
EGFP
ExpressionInsectMutationNix isoforms with only exons 1, 2 separated by in…PromoterAedes aegypti Polyubiquitin and cloned Nix promot…Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2
Plasmid#165831PurposeEBFP2blue fluorescent protein CDSDepositorInsertEBFP2
ExpressionInsectAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTK
Plasmid#165841Purposeganciclovir sensitivity markerDepositorInsertSR39TK
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10
Plasmid#172915PurposeGal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7f-T2A-tdTomato
Tags6xHis and T2A-tdTomatoExpressionInsectPromoterhsp70Available SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Myc-BirA-G3-ER
Plasmid#173815PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
TagsMycExpressionInsectPromoterUASAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)
Plasmid#169185PurposeBaculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cellsDepositorInsertnsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
TagsHis6-3xFlag (nsp12 C-terminus)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
Plasmid#169189PurposeBaculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cellsDepositorInsert3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
Tags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-NbVHH05-GFP
Plasmid#171576PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembraneDepositorInsertCD8-NbVHH05-GFP (UBE2J1 Human)
TagsCD8 and GFPExpressionInsectPromoterfly actin5C promoterAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS-FRT-GFP-FRT-u(M)Cas9
Plasmid#127388PurposeCas9 inducible by removal of a GFP flip-out cassetteDepositorInsertFRT-GFP-FRT-uORF(M)Cas9
UseCRISPRExpressionInsectAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(S)Cas9
Plasmid#127383PurposeExpresses Cas9 at high levelDepositorInsertuORF(S)-Cas9
UseCRISPRExpressionInsectPromoterUASTAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-874PA
Plasmid#164846PurposeExpresses AAEL005635-Cas9 with OpIE2-CFP markerDepositorInsertP10-3'UTR OpIE2-CFP
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-CR7T-gRNA2.1-nlsBFP
Plasmid#170515PurposegRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterCR7TAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9L
Plasmid#166788PurposeInsect cell expression vector for DPP9L (protein purification)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S
Plasmid#166787PurposeInsect cell expression vector for DPP9S (protein purification)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
K20‐scFv‐pSMBP2
Plasmid#159940PurposeTo generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20DepositorInsertanti beta-1 integrin scFv
Tags6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…ExpressionInsectPromoterPolyhedrinAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
47 pLIB_GST
Plasmid#160272PurposeN-term 3C cleavable GST tag for insect cell expressionDepositorTypeEmpty backboneTagsGSTExpressionInsectPromoterpolyhydrinAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
907H1
Plasmid#160234PurposeFlightN-CymR-VP16-3X-SV40-attBDepositorInsertsp-cymene repressor
Flightin promoter
TagsVP16ExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAST-LbCas12a
Plasmid#140621PurposeGal4 dependent expression of LbCas12a in DrosophilaDepositorInsertLbCas12a
UseCRISPRExpressionInsectPromoterUASTAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
52 pLIB_3C_C_STREP
Plasmid#160300PurposeC-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneTags2xStrepIIExpressionInsectPromoterpolyhydrinAvailable SinceNov. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM3
Plasmid#154168PurposeEmpty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorTypeEmpty backboneExpressionInsectAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR
Plasmid#131614PurposeExpresses NeoR and dsRed.DepositorInsertsNeoR
dsRed
ExpressionInsectPromoterOpie2 and hr5Ie1Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-mScarlet-I
Plasmid#138391PurposeRMCE vector for generating C-terminal mScarlet-I fusionDepositorInsertmScarlet-I
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only