78,292 results

1. pcDNA3-Flag mTOR wt

   Plasmid

   #26603

   Purpose

   Mammalian expression of human flag-tagged mTor

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pvPE-V4

   Plasmid

   #240288

   Purpose

   Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

   Depositor

   Lusheng Huang

   Article

   Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

   Insert

   pvPE-V4
   

   Use

   CRISPR

   Tags

   SV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

   Plasmid

   #247160

   Purpose

   Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   Puromycin resistance, mCherry
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1 CMYC

   Plasmid

   #164145

   Purpose

   Doxycycline inducible expression of c-MYC

   Depositor

   Richard Possemato

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Doxycycline inducible promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRT029

   Plasmid

   #127969

   Purpose

   double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKDEL-mScarlet3-H (aka mYongHong)

   Plasmid

   #239411

   Purpose

   Expresses mScarlet3-H (aka mYongHong) localized to ER in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   mScarlet3-H-KDEL
   

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-P4M-SidMx2

   Plasmid

   #51472

   Purpose

   Biosensor for PtdIns4P

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Inserts

   P4M-SidM(546-647)
   P4M-SidM(546-647)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5HT6-G-GECO1.0

   Plasmid

   #47499

   Purpose

   Fluorescent reporter for Ca2+ inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
   

   Tags

   G-GECO1.0

   Expression

   Mammalian

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5_mCherry_P2AT2A_GFP-nb-2xKS

    Plasmid

    #238236

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-2xKS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-2xKS
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. HyPer7.2DAAO-NLS

    Plasmid

    #168302

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5HT6-mCherry-G-GECO1.0

    Plasmid

    #47500

    Purpose

    Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    G-GECO1.0
    

    Tags

    5HT6 and mCherry

    Expression

    Mammalian

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Cre

    Plasmid

    #123133

    Purpose

    Mammalian expression vector for wild-type Cre.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-hSyn1-GFP

    Plasmid

    #177810

    Purpose

    Encodes a GFP driven by human synapsin I promoter

    Depositor

    Christopher Grigsby

    Article

    Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

    Insert

    CopGFP inserted behind human synapsin I promoter (SYN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Syn1

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cre-IRES-PuroR

    Plasmid

    #30205

    Purpose

    Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

    Depositor

    Darrell Kotton

    Article

    Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

    Insert

    Cre recombinase (cre Enterobacteria phage P1)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. (attB) tdMCP-tagged BRaf with MS2-circRNA

    Plasmid

    #226163

    Purpose

    Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    3xFlag-tdMCP-BRaf (BRAF Human)
    IRES-mCherry-P2A-PuroR
    MS2-circRNA barcode
    

    Use

    Bxb1 attb, no promoter

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid

    #139988

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1-FOXM1b

    Plasmid

    #68811

    Purpose

    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

    Depositor

    Adam Karpf

    Article

    Barger et al Oncotarget. 2015 Jul 16.

    Insert

    FOXM1b (FOXM1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(-)-WIF1

    Plasmid

    #99533

    Purpose

    WIF1 expression plasmid

    Depositor

    Qian Tao

    Article

    Chan et al Lab Invest. 2007 Jul;87(7):644-50. Epub

    Insert

    WIF1 (WIF1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG PSAM4-5HT3 HC IRES EGFP

    Plasmid

    #119740

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Human, Synthetic)
    

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F, R420Q, R424D, R428A

    Promoter

    CAG

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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