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Mammalian Expression

76,965 results

Showing: 201 - 220 of 76965 results

  4. EGFP-GRAM-W

    Plasmid
    #211701
    Purpose
    Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211701-plasmid-map-sequence-id-419225

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  8. pC0043-PspCas13b crRNA backbone

    Plasmid
    #103854
    Purpose
    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6-BbsI-BbsI-PspCas13b DR-polyT
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 28, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    103854-plasmid-map-sequence-id-350782

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  10. pCW57.1

    Plasmid
    #41393
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41393-plasmid-map-sequence-id-364440

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  11. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid
    #137830
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Article
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Insert
    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Leucine 71 to Lysine in CTEVp fragment
    Promoter
    CMV
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137830-plasmid-map-sequence-id-311956

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  14. pcDNA3.1-HA

    Plasmid
    #128034
    Purpose
    Mammalian expression vector with N terminal HA tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128034-plasmid-map-sequence-id-422971

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  16. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid
    #221359
    Purpose
    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221359-plasmid-map-sequence-id-436820

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  17. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

    Plasmid
    #221360
    Purpose
    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221360-plasmid-map-sequence-id-436821

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  18. Flag-TurboID

    Plasmid
    #124646
    Purpose
    Expresses Flag-TurboID fusion for proximity labeling
    Depositor
    Ken-Ichi Takemaru, FENG-QIAN Li
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124646-plasmid-map-sequence-id-435118

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  19. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44248-plasmid-map-sequence-id-190219

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Showing: 201 - 220 of 76965 results