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pCAG-G-Flamp2

Plasmid

#192782

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-mt-mKeima-P2A-FRB-Fis1

Plasmid

#135295

Purpose

stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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NSUN2

Plasmid

#155793

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NSUN2 (NSUN2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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RNb-GFP

Plasmid

#128777

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-GFP

Expression

Mammalian

Available Since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-B/BRISBANE/60/2008

Plasmid

#187920

Purpose

Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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RED

Plasmid

#139835

Purpose

Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))

Expression

Mammalian

Mutation

dCas9 protein contains two mutations: D10A and H8…

Promoter

tet-inducible CMV

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-GFP-nb-KS

Plasmid

#238260

Purpose

For overexpression of mCherry-GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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VPS13A^mCherry

Plasmid

#118758

Purpose

VPS13A internally tagged with mCherry at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

mCherry inserted at position 1372

Expression

Mammalian

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-IGHG1

Plasmid

#80795

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DDX4

Plasmid

#222527

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DDX4 (DDX4 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCGT003_nuclear-mKate2

Plasmid

#248085

Purpose

Constitutive expression of NLS-tagged mKate2 from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Article

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

mKate2

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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vYM140_PB-pEFa1-iaaM(P.s)-Hygro

Plasmid

#160043

Purpose

Expressing iaaM for converting L-Trp to IAM

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

iaaM (iaaM )

Use

Synthetic Biology

Expression

Mammalian

Promoter

pEF1a

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pNX24-RFP-MS2SL

Plasmid

#227819

Purpose

Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

3xHA-RFP-24xMS2SL
Neo resistance

Expression

Mammalian

Promoter

CMV and SV40

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMIG-w

Plasmid

#12282

Purpose

a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus

Depositor

Luk Parijs

Article

Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.

Type

Empty backbone

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 28, 2006

Availability

Academic Institutions and Nonprofits only

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BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

Plasmid

#235087

Purpose

GFP splicing reporter with FRG1 intron

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)

Use

AAV

Expression

Mammalian

Promoter

custom bidirectional

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1

Plasmid

#211578

Purpose

CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-Cre

Plasmid

#198712

Purpose

For lentiviral expression of Cre recombinase in mammalian cells.

Depositor

Tyler Jacks

Article

Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

Insert

Pgk-Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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VPS13B^GFP

Plasmid

#224588

Purpose

codon optimized GFP tagged VPS13B for expression in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

VPS13B^GFP (VPS13B Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-WT-WLS-HA-IRES-GFP

Plasmid

#178061

Purpose

Expression of WLS-HA protein (WT)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

Plasmid

#166970

Purpose

Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.

Depositor

Matthew Banghart

Article

Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)

Use

AAV and Cre/Lox

Tags

FLAG, T2A, and mCherry

Expression

Mammalian

Promoter

hsyn

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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