75,007 results

1. mRFP-Rab7

   Plasmid

   #14436

   Purpose

   Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 7 (RAB7A Canis familialis)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   3 silent mutations near the C-terminus

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-EglN1-pcDNA3

   Plasmid

   #18963

   Depositor

   William Kaelin

   Article

   Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

   Insert

   Egg laying Nine - 1 (EGLN1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti EF1a-FLAG-dCas9-VPR

   Plasmid

   #114195

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LDB-3-p65

   Plasmid

   #158984

   Purpose

   Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

   Depositor

   Liangcai Gu

   Article

   Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

   Insert

   LDB-3-p65
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Thy1.1-GFP

   Plasmid

   #131703

   Purpose

   encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

   Depositor

   Britt Glaunsinger

   Article

   Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

   Insert

   Thy1.1-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. VAMP3-mCherry

   Plasmid

   #92423

   Purpose

   Expression of VAMP3 C-terminally conjugated to mCherry.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   VAMP3 (Vamp3 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GPR65-Tango

   Plasmid

   #66370

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR65 (GPR65 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. p23-puro-EGFP-RPA194

   Plasmid

   #179237

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   RPA194 (POLR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST-CMV mCherry-GFP-LC3B G120A

   Plasmid

   #123235

   Purpose

   Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   CMV

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXL010-Wnt dual (GFP-Fire) reporter

    Plasmid

    #40588

    Depositor

    Sean Palecek

    Article

    Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

    Insert

    Wnt reporter sequences
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Wnt promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3 NQO1-Flag

    Plasmid

    #61729

    Purpose

    Expresses human NQO1 Flag tagged at C-terminus

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al J Biol Chem. 2011 Mar 18;286(11):8839-45

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA GFP10-ZIP-GFP11

    Plasmid

    #182243

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP-11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLD-puro-MTS-EGFP

    Plasmid

    #190270

    Purpose

    Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

    Depositor

    Christian Münch

    Article

    Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

    Insert

    MTS-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psi-CHECK3

    Plasmid

    #136010

    Purpose

    psi-CHECK2 plasmid modified so both Renilla and Firefly luciferase contain introns

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCG_IFITM1

    Plasmid

    #179955

    Purpose

    Expression vector for human IFITM1.

    Depositor

    Konstantin Sparrer, Frank Kirchhoff

    Article

    Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

    Insert

    IFITM1 (IFITM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P13A

    Promoter

    Available since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)-blueAKAR

    Plasmid

    #118470

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5FRT/TO-UBXD3-Strep-HA

    Plasmid

    #113488

    Purpose

    Expression of human UBXD3 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD3 (UBXN10 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF6-CSPG4-myc-his

    Plasmid

    #69037

    Purpose

    Expression of CSPG4 in mammalian cells

    Depositor

    Wensheng Wei

    Article

    Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

    Insert

    CSPG4 (CSPG4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1 alpha

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge