78,292 results

1. mEmerald-Sec61b-C1

   Plasmid

   #90992

   Purpose

   Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mEmerald-Sec61b (SEC61B Human, Synthetic)
   

   Tags

   mEmerald (green fluorescence)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-CAGGS-dCas9-KRAB-MeCP2

   Plasmid

   #110824

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE GFP

   Plasmid

   #10668

   Purpose

   Empty backbone for retroviral gene expression. Select recipient cells using GFP.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmini-iCAP-AAV9-X1.1

   Plasmid

   #196836

   Purpose

   non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

   Insert

   Synthetic construct isolate AAV9-X1.1 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTagRFP-C1-Fibrillarin

   Plasmid

   #70649

   Purpose

   Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   TagRFP

   Expression

   Mammalian

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPRv2FE-ABE8e-SpRY

   Plasmid

   #213008

   Purpose

   A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE-puro-hTERT

   Plasmid

   #1771

   Purpose

   Retroviral expression of hTERT. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

   Insert

   hTERT (TERT Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. T7-VEE-IRES-Puro

   Plasmid

   #58970

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-Q74

    Plasmid

    #40262

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1(+)-CMV-bArrestin2-TEV

    Plasmid

    #107245

    Purpose

    Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ARRB2
    

    Tags

    TEV(NIA), tobacco etch virus NIA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti sfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164478

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry-2xFYVE

    Plasmid

    #140050

    Purpose

    Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2/6

    Plasmid

    #240485

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein

    Depositor

    Meghan Rego, Addgene Research Program

    Insert

    AAV2 Rep and AAV6 Cap
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

    Plasmid

    #223712

    Purpose

    Expresses autophagy flux LC3B fluorescence reporter

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    LC3B (MAP1LC3B Human, Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    mCherry::EGFP::LC3B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid

    #72486

    Purpose

    Express Luc2 and tdTomato under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCmini-iCAP-PHP.eB

    Plasmid

    #103005

    Purpose

    non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    Synthetic construct isolate AAV-PHP.eB VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CMV-cGAS-HA

    Plasmid

    #130910

    Purpose

    Expresses human cGAS-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE

    Plasmid

    #226381

    Purpose

    Expresses tau propagation ORF with V5 tag on WT 2N4R tau

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Tags

    TagRFP.T.2A.V5

    Expression

    Mammalian

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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