81,213 results

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GFP-LKB1

Plasmid

#21147

Depositor

Junying Yuan

Article

Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

Insert

GFP-LKB1 (Stk11 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

Plasmid

#12153

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Retroviral

Tags

6XHis

Expression

Mammalian

Available Since

July 7, 2006

Availability

Academic Institutions and Nonprofits only

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p181 pK7-HDAC1 (GFP)

Plasmid

#11054

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV9-X1.1

Plasmid

#196836

Purpose

non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV9-X1.1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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Sec61b-V5-TurboID

Plasmid

#166971

Purpose

Expresses ER targeted TurboID for secretome biotinylation

Depositor

Hyun-Woo Rhee

Article

Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

Insert

Sec61b-V5-TurboID

Tags

V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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PyronicSF/pcDNA3.1(-)

Plasmid

#124812

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-mCh-Cry2olig

Plasmid

#101224

Purpose

FUS(1-214) fused to mCh-Cry2olig

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2olig

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D1R

Expression

Mammalian

Promoter

CAG promotor

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_C1

Plasmid

#85043

Purpose

Mammalian expression of mScarlet-H

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

Plasmid

#74928

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-kif18b-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMK288 (mAID-Bsr)

Plasmid

#72826

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Bsr

Use

CRISPR

Tags

mAID

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLPCX mito roGFP2-Orp1

Plasmid

#64992

Purpose

Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cry2FL-VP64

Plasmid

#60554

Purpose

Expresses Cry2FL-VP64 in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

Cry2FL-VP64

Tags

FLAG, HA tag, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-CRY2-mCherry

Plasmid

#58368

Purpose

Mammalian codon optimized CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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miniSOG-C1

Plasmid

#54821

Purpose

Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-LTR-dCas9-p65AD-BFP

Plasmid

#46913

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-p65AD-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

2xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-AID-BubR1

Plasmid

#47330

Purpose

for plasmid integration using the Flp-In system. AID at N-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)

Tags

AID and GFP

Expression

Mammalian

Mutation

siRNA resistant

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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piRFP682-N1

Plasmid

#45459

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP682

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Puromycin-mVenus-MAP

Plasmid

#44118

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Tags

mVenus-MAP

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pWPXL-Sox9

Plasmid

#36979

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC1

Plasmid

#35680

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pLenti6-MK1-EHMT2-V5

Plasmid

#31113

Depositor

Bernard Futscher

Insert

euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Stop codon mutated to Ala

Available Since

Aug. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag-JunB

Plasmid

#29687

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

jun B oncogene (JUNB Human)

Tags

Flag

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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mouse Drp1(K38A)

Plasmid

#26049

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Drp1 (Dnm1l Mouse)

Expression

Mammalian

Mutation

K38A GTPase mutant

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pLKO-RB1-shRNA63

Plasmid

#25641

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 63 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1-C450A

Plasmid

#22028

Purpose

Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiFC-CC155

Plasmid

#22015

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

CC155

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge

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81,213 results