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Showing: 751 - 800 of 3935 results
  1. pdRBFC-B2-YN

    Plasmid
    #74721
    Purpose
    Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling
    Depositor
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    HA and YFP N-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pOT2-poly-cis

    Plasmid
    #141177
    Purpose
    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter
    Depositor
    Type
    Empty backbone
    Use
    RNAi and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S promoter
    Available since
    Oct. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. PROM_MpUBE2

    Plasmid
    #136109
    Purpose
    PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.
    Depositor
    Insert
    PROM_MpUBE2 Mapoly0106s0019
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. 5UTR_MpUBE2

    Plasmid
    #136110
    Purpose
    5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.
    Depositor
    Insert
    5UTR_MpUBE2 Mapoly0106s0019
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pUB-Cas9

    Plasmid
    #86556
    Purpose
    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
    Depositor
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Cas9 is codon optimized for C. reinhardtii
    Promoter
    UBIQUITIN10
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pdRBFC-B2-YC

    Plasmid
    #74722
    Purpose
    Express C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling
    Depositor
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    FLAG and YFP C-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. P305-FBP_4

    Plasmid
    #139695
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid
    #197952
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.
    Depositor
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid
    #211845
    Purpose
    Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.
    Inserts
    LRR-NEL
    GAL4-VP16-GR
    Use
    Synthetic Biology
    Tags
    HA, Myc, and mCherry
    Expression
    Plant
    Mutation
    C492A
    Promoter
    6xUAS-35Smini and pUBQ10
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pDP085

    Plasmid
    #198425
    Purpose
    pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120
    Depositor
    Insert
    E. coli BirA
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pUBQ10
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pYPQ230-RRVQ

    Plasmid
    #200188
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing
    Depositor
    Insert
    LbCas12a-RRVQ
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V, E795Q
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)

    Plasmid
    #160634
    Purpose
    TU for wtGFP expression driven by 2x35s promoter.
    Depositor
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    2x35s
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pYPQ-SpRY-Act3.0

    Plasmid
    #178957
    Purpose
    It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.
    Depositor
    Insert
    SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pE5c

    Plasmid
    #17465
    Purpose
    modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest
    Depositor
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    ECFP
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
  16. pE2n

    Plasmid
    #17456
    Purpose
    modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest
    Depositor
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    3xHA
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
  17. pSpG-PPE

    Plasmid
    #170130
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Insert
    nCas9-SpG(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…
    Promoter
    maize Ubiquitin-1
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 751 - 800 of 3935 results