4,307 results

1. tRNA-gRNA position [2_n-1] (GB1206)

   Plasmid

   #75407

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [2_n-1]
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJG493

   Plasmid

   #91197

   Purpose

   WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gUbi1+gUbi8+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMU103

   Plasmid

   #73934

   Purpose

   This is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.

   Depositor

   Zhanyuan Zhang

   Article

   Flores et al Transgenic Res. 2008 Oct;17(5):839-50. d

   Insert

   bar and GUS cassettes
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZA71

   Plasmid

   #174785

   Purpose

   In planta gene expression

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker
   

   Use

   Tags

   3xFLAG

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZA68

   Plasmid

   #174781

   Purpose

   In planta gene expression

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker
   

   Use

   Tags

   eGFP

   Expression

   Plant

   Mutation

   L17E/D481V

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZA66

   Plasmid

   #174779

   Purpose

   In planta gene expression

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker
   

   Use

   Tags

   eGFP

   Expression

   Plant

   Mutation

   D481V

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZA65

   Plasmid

   #174778

   Purpose

   In planta gene expression

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-eGFP, Kmr plant selection marker
   

   Use

   Tags

   eGFP

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZA67

   Plasmid

   #174780

   Purpose

   In planta gene expression

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker
   

   Use

   Tags

   eGFP

   Expression

   Plant

   Mutation

   L17E

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

   Plasmid

   #160635

   Purpose

   TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

   Insert

   AcrIIA4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRATIO2251

    Plasmid

    #141305

    Purpose

    Gateway cloning compatible ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    Firefly Luciferase or LUC2 and mScarlet-I

    Expression

    Plant

    Mutation

    Promoter

    UBQ10 promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMpGWB106-OEP7(1–50)

    Plasmid

    #100602

    Purpose

    Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

    Depositor

    Yutaka Kodama

    Article

    Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

    Insert

    OEP7(1–50)-Citrine (OEP7 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)

    Plasmid

    #160641

    Purpose

    Module for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    ERLexABDGal4AD / PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pG20_LUC2_Hyg

    Plasmid

    #159707

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Use

    Tags

    luciferase

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pG20_GUS_Blp

    Plasmid

    #159706

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEG302 5aa SunTag VP64 g4+g17 (FWA)

    Plasmid

    #119672

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPOR1CB0112

    Plasmid

    #117548

    Purpose

    Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEE386

    Plasmid

    #149284

    Purpose

    T-DNA encoding TRV2 with gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEE388

    Plasmid

    #149286

    Purpose

    T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_mFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

19. pEE385

    Plasmid

    #149289

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PABE-2

    Plasmid

    #115623

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pG20_GUS_Hyg

    Plasmid

    #159705

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGREAT4

    Plasmid

    #170892

    Purpose

    GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pG10-90

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMpGWB221

    Plasmid

    #68612

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFGC5941-PacI-ath-STTM162

    Plasmid

    #84088

    Purpose

    target miRNA162 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM162
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMpGWB330

    Plasmid

    #68658

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMpGWB319

    Plasmid

    #68647

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEL065

    Plasmid

    #124896

    Purpose

    For genome editing in plant by SpCas9 variant(SpCas9-NGv1)

    Depositor

    Yong Zhang

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lvl0 ACT2p

    Plasmid

    #153374

    Purpose

    Plant promoter

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    ACT2 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lvl0 RbcS2bt

    Plasmid

    #153387

    Purpose

    Plant terminator

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    RbcS2b terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDGE3

    Plasmid

    #79442

    Purpose

    recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDGE64

    Plasmid

    #79446

    Purpose

    recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDGE1

    Plasmid

    #79440

    Purpose

    recipient plasmid [multiplexing]; p35S:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTaCas9_5

    Plasmid

    #91168

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAtCas9-H840A_2

    Plasmid

    #91163

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_H840A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SUN2delSUN

    Plasmid

    #130835

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    SUN2
    

    Use

    Tags

    mCherry-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of SUN domain

    Promoter

    CaMV 35S

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. lvl0 TUB9t

    Plasmid

    #153385

    Purpose

    Plant terminator

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    TUB9 terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lvl0 35St

    Plasmid

    #153382

    Purpose

    Cauliflower mosaic virus terminator for plant expression

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    35S terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEPOR2KN0038

    Plasmid

    #117605

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUBQ10::ZF1CACTA1_3xFLAG_TET1cd

    Plasmid

    #106434

    Purpose

    pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMDC84-SEIPIN3-Ndelta

    Plasmid

    #96981

    Purpose

    Express N-terminal truncated Arabidopsis SEIPIN3 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN3 (AT2G34380 Mustard Weed)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    A segment of 507 nucleotides are deleted from the…

    Promoter

    35S

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge