1,779 results

1. Pmec-18 cz::ced-3 (p17) [TU#807]

   Plasmid

   #16081

   Depositor

   Martin Chalfie

   Article

   Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

   Insert

   cz:: ced-3 (p17) (ced-3 Nematode)
   

   Expression

   Worm

   Available Since

   June 23, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNM3725

   Plasmid

   #154113

   Purpose

   UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   UAS 11X::∆pes-10::GFP-C1::tbb-2 3'
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV1825

   Plasmid

   #37838

   Depositor

   Gary Silverman

   Article

   Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

   Insert

   Pnhx-2DsRed::KDEL
   

   Tags

   KDEL

   Expression

   Worm

   Promoter

   nhx-2

   Available Since

   Dec. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pHT101-mCherry

   Plasmid

   #61021

   Purpose

   Expresses mCherry tagged fusion proteins in worms

   Depositor

   Casonya Johnson

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Worm

   Promoter

   lac

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW2172

   Plasmid

   #163096

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

   Plasmid

   #182348

   Purpose

   mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1586

   Plasmid

   #121057

   Purpose

   mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mKate2-T^SEC^AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, C. elegans codon optimized mKate2, and au…

   Expression

   Worm

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD49_78

   Plasmid

   #1447

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   hsp16-415', hsp16-25', IVSA, rf2128

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM3 = ncs-1p::nCre

   Plasmid

   #24034

   Depositor

   Cori Bargmann

   Article

   Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

   Insert

   ncs-1 (ncs-1 Nematode)
   

   Use

   Cre/Lox

   Tags

   nCre

   Expression

   Worm

   Available Since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH1579

    Plasmid

    #178904

    Purpose

    Pglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60

    Depositor

    Mei Zhen

    Article

    Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

    Insert

    YC3.60
    

    Expression

    Worm

    Mutation

    See Depositor Comments Below

    Promoter

    Pglr-1

    Available Since

    Jan. 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBN154

    Plasmid

    #86718

    Purpose

    FLP/Frt dual color reporter for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
    

    Expression

    Worm

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJW1836

    Plasmid

    #154337

    Purpose

    Promoter reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR
    

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

    Plasmid

    #193854

    Purpose

    Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' Cbr-unc-119(+) + 5' mNeonGreen
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Promoter

    Cbr-unc-119p

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD363

    Plasmid

    #91829

    Purpose

    LoxP-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    LoxP-SEC-LoxP
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF11-wmiRFP670-2-T2A-HO1

    Plasmid

    #197250

    Purpose

    Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP670-2-T2A-HO1
    

    Expression

    Worm

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDD379

    Plasmid

    #91834

    Purpose

    SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGLOW39

    Plasmid

    #173068

    Purpose

    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    mScarlet-I-C1^SEC^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mScarlet

    Expression

    Worm

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSM08

    Plasmid

    #139042

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    mscarlet (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMLS134

    Plasmid

    #73714

    Purpose

    SapTrap destination vector for building sgRNA expression vectors.

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    pU6::sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    SapI insertion site

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOL2339

    Plasmid

    #37839

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Ub-V
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    nhx-2

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOL2340

    Plasmid

    #37841

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Ub-M
    

    Tags

    mCherry

    Expression

    Worm

    Mutation

    R77M

    Promoter

    nhx-2

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJW1347

    Plasmid

    #154310

    Purpose

    Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa Linker for CT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107132

    Purpose

    cGAL-N driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Expression

    Worm

    Promoter

    C. elegans eft-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

    Plasmid

    #107136

    Purpose

    cGAL-C driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Expression

    Worm

    Promoter

    C. elegans eft-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCC324

    Plasmid

    #110165

    Purpose

    miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    R61K change made in ERK-KTR CDS

    Promoter

    lin-31 promoter/enhancer

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAS3-rab-3P::AI::ChR2(H134R)

    Plasmid

    #232601

    Purpose

    Pan-neuronal expression of opsin 'ChR2(H134R)', regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    Channelrhodopsin2
    

    Expression

    Worm

    Mutation

    H134R

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCFJ150-GFP(dpiRNA)::CDK-1

    Plasmid

    #107938

    Purpose

    cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
    

    Expression

    Worm

    Promoter

    cdk-1

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFJ178 - cxTi10882_MCS

    Plasmid

    #19331

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    cxTi10882 targetting region
    

    Expression

    Worm

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

    Plasmid

    #159803

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXF276

    Plasmid

    #139048

    Purpose

    MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    lov domain (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge