1,755 results

1. pDD382

   Plasmid

   #91830

   Purpose

   Lox2272-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox2272-SEC-Lox2272
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJR021

   Plasmid

   #198814

   Purpose

   Variant of yellow fluorescent protein for transient gene expression

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::destabilizedYFP::let-838 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   YFP with destabilized signal from mouse ornithine…

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHW450

   Plasmid

   #198831

   Purpose

   Voltage-sensitive fluorescent protein.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV103

   Plasmid

   #63193

   Purpose

   pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Worm and Yeast

   Mutation

   Promoter

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSF11-wemiRFP670-T2A-HO1

   Plasmid

   #197243

   Purpose

   Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wemiRFP670-T2A-HO1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BSP610

   Plasmid

   #122255

   Purpose

   expression vector for mtagBFP2 under EFT3 promoter

   Depositor

   Alexander Mendenhall

   Article

   Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

   Insert

   mtagBFP2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   EFT3

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sgRNA with U6 promoter

   Plasmid

   #48962

   Purpose

   to drive the sgRNA expression under a U6 promoter

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNM3814

   Plasmid

   #154122

   Purpose

   mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   mec-4p::rtetR-L-QFAD::act-4 '3
   

   Use

   Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEft-3::Cerulean-Venus::Tomm-7

   Plasmid

   #68046

   Purpose

   Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

   Depositor

   Dana Miller

   Article

   Chapin et al Aging (Albany NY). 2015 Jun 30.

   Insert

   pEft-3::Cerulean-Venus::Tomm-7
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ178 - cxTi10882_MCS

    Plasmid

    #19331

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    cxTi10882 targetting region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. XW12

    Plasmid

    #65834

    Purpose

    Pquas-Δpes10-GFP-3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    5XQUAS-delta-pes-10
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDD121

    Plasmid

    #91833

    Purpose

    Cas9 expression in C. elegans

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHZ081

    Plasmid

    #27506

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pcol-10::lin-4::let-858_3'UTR (lin-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJH3449

    Plasmid

    #179576

    Purpose

    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    BFP

    Expression

    Worm

    Mutation

    Promoter

    Plgc-55B

    Available since

    March 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

    Plasmid

    #159849

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCFJ1955

    Plasmid

    #84830

    Purpose

    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized tdTomato
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Peft-3

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKS2301

    Plasmid

    #37834

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2cpl-1::YFP (cpl-1 Nematode)
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    nhx-2

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159814

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNM3724

    Plasmid

    #154112

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJW1583

    Plasmid

    #121054

    Purpose

    GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    GFP^SEC^AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFLAG, C. elegans codon optimized GFP, and auxin…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJS145

    Plasmid

    #179159

    Purpose

    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

    Depositor

    Iva Greenwald

    Article

    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Insert

    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Flexon replacing 2nd intron of 2xNLS::GFP

    Promoter

    rps-27p

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDD04neo pmyo2::gfp

    Plasmid

    #26393

    Depositor

    Denis Dupuy

    Article

    Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBCN27-Pmyo-2::GFP::unc-54_3'UTR

    Plasmid

    #26347

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

    Insert

    Pmyo-2::GFP::unc-54_3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBN396

    Plasmid

    #109326

    Purpose

    Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

    Depositor

    Peter Askjaer

    Article

    Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

    Insert

    mex-5 (mex-5 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    mex-5

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCFJ352

    Plasmid

    #30539

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

    Plasmid

    #182351

    Purpose

    Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJH4542

    Plasmid

    #200779

    Purpose

    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GCaMP6
    

    Use

    Tags

    mNeptune

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pDD378

    Plasmid

    #91827

    Purpose

    Codon-optimized HaloTag donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    HaloTag-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJW1839

    Plasmid

    #154325

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BSP605

    Plasmid

    #122250

    Purpose

    expression vector for mCerulean under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mCerulean
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge