1,779 results

1. pJF5

   Plasmid

   #139031

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   gfp (no STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJH4723

   Plasmid

   #178899

   Purpose

   Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of of DAF-2

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-2 (daf-2 Nematode)
   

   Expression

   Worm

   Promoter

   Pges-1

   Available Since

   Jan. 5, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pXF88

   Plasmid

   #139039

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   halotag (includes STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgRNA with U6 promoter

   Plasmid

   #48962

   Purpose

   to drive the sgRNA expression under a U6 promoter

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. XW12

   Plasmid

   #65834

   Purpose

   Pquas-Δpes10-GFP-3'UTR-unc-54

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   5XQUAS-delta-pes-10
   

   Tags

   GFP

   Expression

   Worm

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV103

   Plasmid

   #63193

   Purpose

   pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Worm and Yeast

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH23

   Plasmid

   #159314

   Purpose

   Punc-25 GFP::SYD-2

   Depositor

   Mei Zhen

   Article

   Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

   Insert

   syd-2 (syd-2 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Punc-25

   Available Since

   Sept. 22, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCFJ1955

   Plasmid

   #84830

   Purpose

   Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized tdTomato
   

   Expression

   Bacterial and Worm

   Promoter

   Peft-3

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

   Plasmid

   #193852

   Purpose

   Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   Guide RNA
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDD04neo pmyo2::gfp

    Plasmid

    #26393

    Depositor

    Denis Dupuy

    Article

    Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

    Type

    Empty backbone

    Expression

    Bacterial and Worm

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BSP610

    Plasmid

    #122255

    Purpose

    expression vector for mtagBFP2 under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mtagBFP2
    

    Expression

    Worm

    Promoter

    EFT3

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSDH52

    Plasmid

    #139049

    Purpose

    MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    epdz (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEft-3::Cerulean-Venus::Tomm-7

    Plasmid

    #68046

    Purpose

    Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

    Depositor

    Dana Miller

    Article

    Chapin et al Aging (Albany NY). 2015 Jun 30.

    Insert

    pEft-3::Cerulean-Venus::Tomm-7
    

    Expression

    Worm

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L3829

    Plasmid

    #1604

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, lacZN

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJW1583

    Plasmid

    #121054

    Purpose

    GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    GFP^SEC^AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFLAG, C. elegans codon optimized GFP, and auxin…

    Expression

    Worm

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCFJ352

    Plasmid

    #30539

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Expression

    Worm

    Available Since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNM3814

    Plasmid

    #154122

    Purpose

    mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::rtetR-L-QFAD::act-4 '3
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBCN27-Pmyo-2::GFP::unc-54_3'UTR

    Plasmid

    #26347

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

    Insert

    Pmyo-2::GFP::unc-54_3'UTR
    

    Expression

    Worm

    Available Since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJRK136

    Plasmid

    #173744

    Purpose

    CeLINC plasmid

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    Prps-0::CIBN-3xFLAG::MP::unc-54 3' UTR
    

    Expression

    Worm

    Promoter

    Prps-0

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSDH60

    Plasmid

    #139029

    Purpose

    MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    spe-11 promoter (spe-11 Nematode)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159814

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDD121

    Plasmid

    #91833

    Purpose

    Cas9 expression in C. elegans

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. L4817

    Plasmid

    #1683

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    myo3-2341, myo3Tsa, gfpN, IVSR, gfpGLA, IVSS, IVS…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJRK138

    Plasmid

    #173746

    Purpose

    CeLINC plasmid

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    Prps-0::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
    

    Expression

    Worm

    Promoter

    Prps-0

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDD364

    Plasmid

    #91831

    Purpose

    Lox511I-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox511I-SEC-Lox511I
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJH4203

    Plasmid

    #200160

    Purpose

    Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    twk-40 (twk-40 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prig-3

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pJJR81

    Plasmid

    #75029

    Purpose

    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Insert

    tagBFP2^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized tagBFP2

    Expression

    Worm

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNM3724

    Plasmid

    #154112

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDD382

    Plasmid

    #91830

    Purpose

    Lox2272-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox2272-SEC-Lox2272
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PU6::klp-12_sgRNA

    Plasmid

    #46170

    Purpose

    klp-12 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    klp-12 targeting sgRNA (klp-12 Synthetic)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available Since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge