1,755 results

1. pJF6

   Plasmid

   #139040

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   gfp (includes STOP codon, PATC introns)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKS235

   Plasmid

   #191855

   Purpose

   Negative selection marker for MosSCI

   Depositor

   K Subramaniam

   Article

   Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

   Insert

   peel-1 toxic protein for negative selection against extrachromosomal array
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.41

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJJR81

   Plasmid

   #75029

   Purpose

   tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Insert

   tagBFP2^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized tagBFP2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ChrII_ttTi5605_loxP_myo2_neoR

   Plasmid

   #194056

   Purpose

   Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ttTi5605
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAS1-gpa-2P::his-24::Cerulean

   Plasmid

   #124337

   Purpose

   Multicolor Worms for Neuronal ID

   Depositor

   Andrew Leifer

   Insert

   gpa-2, Cerulean (gpa-2 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PU6::klp-12_sgRNA

   Plasmid

   #46170

   Purpose

   klp-12 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   klp-12 targeting sgRNA (klp-12 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS158

   Plasmid

   #215679

   Purpose

   Empty repair plasmid for ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

   Plasmid

   #66753

   Purpose

   miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

   Depositor

   Yishi Jin

   Article

   Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   miniSOG
   

   Use

   Tags

   tomm-20 (N terminal 55 aa)

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSR49

   Plasmid

   #69259

   Purpose

   phlh-8::tagBFP2

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   TagBFP2
   

   Use

   Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0, TagBFP2 contains 2 sing…

   Promoter

   hlh-8

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB16 (pmyo-3::QuasAr)

    Plasmid

    #130272

    Purpose

    Expresses the voltage sensor QuasAr in BWMs of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    QuasAr
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ChrV_oxTi365_loxP_myo2_neoR

    Plasmid

    #194057

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    oxTi365
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJW2341

    Plasmid

    #186950

    Purpose

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

    Depositor

    Jordan Ward

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159813

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRB1080

    Plasmid

    #71309

    Purpose

    Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

    Depositor

    Andrew Fire

    Article

    Bell et al Genetics. 2015 Dec 17. pii: genetics.115

    Insert

    VQR Cas9 variant
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAS1-lim-4P::his-24::Cerulean

    Plasmid

    #124336

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    lim-4, Cerulean (lim-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ108 - pENTR221 - [cbr-unc-119(+)]

    Plasmid

    #200367

    Purpose

    cbr-unc-119 rescue gene for use as co-injection marker.

    Depositor

    Christian Froekjaer-Jensen

    Article

    AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

    Insert

    [cbr-unc-119(+)]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159850

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJH127

    Plasmid

    #162483

    Purpose

    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

    Depositor

    Bob Goldstein

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    

    Use

    CRISPR

    Tags

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMLS716

    Plasmid

    #188888

    Purpose

    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    floxed Cbr-unc-119 marker and Phsp16.41::Cre
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHygCG2r

    Plasmid

    #200074

    Purpose

    hygR selection mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. XW09

    Plasmid

    #65833

    Purpose

    Punc-4c-QS-SL2-mcherry3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Neurospora crassa OR74A repressor (qa-1S)
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-4c

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJH3626

    Plasmid

    #179630

    Purpose

    Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    RFP

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2(s)

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMS050

    Plasmid

    #91826

    Purpose

    Codon-optimized mScarlet-I donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mScarlet-I-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCFJ150-set2

    Plasmid

    #82546

    Purpose

    Pmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::set-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMB70

    Plasmid

    #47943

    Purpose

    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHW581

    Plasmid

    #198818

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGC185

    Plasmid

    #19624

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

    Plasmid

    #182358

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pmex-5

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJA281

    Plasmid

    #22513

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    Pmex-5::mCherry (w introns w/o stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge