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Showing: 601 - 650 of 1735 results

  1. pJW1595

    Plasmid
    #121062
    Purpose
    mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121062-plasmid-map-sequence-id-235983

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  13. pJH126

    Plasmid
    #162482
    Purpose
    SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162482-plasmid-map-sequence-id-320829

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  14. pNM3749

    Plasmid
    #154108
    Purpose
    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    Use
    Cre/Lox; Rmce
    Tags
    Expression
    Worm
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154108-plasmid-map-sequence-id-306067

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  16. pPD93_48

    Plasmid
    #1473
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc54-204, unc545', IVSA, SV40NLS, gfpN, IVS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1473-plasmid-map-sequence-id-84

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  19. pCC336

    Plasmid
    #110167
    Purpose
    miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    Phospho-acceptor sites mutated: S43E, T55E, T62E.…
    Promoter
    lin-31 promoter/enhancer
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110167-plasmid-map-sequence-id-238139

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  20. pCC335

    Plasmid
    #110166
    Purpose
    miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    Phospho-acceptor sites mutated: S43A, T55A, T62A.…
    Promoter
    lin-31 promoter/enhancer
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110166-plasmid-map-sequence-id-235342

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  21. pSAH85 - [AWCp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200359
    Purpose
    mScarlet BioPart for AWC neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [AWCp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00012770, srt-47
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200359-plasmid-map-sequence-id-397601

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  23. pAC32 [TU#839]

    Plasmid
    #16079
    Depositor
    Martin Chalfie
    Insert
    mec-2 intron 9
    Use
    Tags
    YFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 23, 2008
    Availability
    Academic Institutions and Nonprofits only
    16079-plasmid-map-sequence-id-8472

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  24. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid
    #85820
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc Dam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A.
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85820-plasmid-map-sequence-id-170474

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  25. XW54

    Plasmid
    #65839
    Purpose
    Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method
    Depositor
    Kang Shen
    Article
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    NLS-QF-BD-DM-Zipper
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    Promoter
    Punc-4c
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    65839-plasmid-map-sequence-id-125186

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  27. pPD117.20

    Plasmid
    #1712
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc37-448, unc37Tsa, gfpN, IVSR, gfpF64L, gfpS65T…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1712-plasmid-map-sequence-id-318

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  34. L4667

    Plasmid
    #1669
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, IVSA, rf2128, ATG-MitLS, gfpN…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1669-plasmid-map-sequence-id-279

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  37. pSAH51 - [DDp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200327
    Purpose
    mScarlet BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [DDp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00007304, ttr-39
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200327-plasmid-map-sequence-id-397610

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  38. XW17

    Plasmid
    #65835
    Purpose
    Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)
    Depositor
    Kang Shen
    Article
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    OR74A quinic acid utilization activator (qa-1F)
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    The endogenous SphI, KpnI and SacI sites in QF ar…
    Promoter
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65835-plasmid-map-sequence-id-120565

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  44. pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid
    #159855
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    159855-plasmid-map-sequence-id-326622

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  46. L4043

    Plasmid
    #1624
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    hsp16-415', hsp16-25', IVSA, SV40NLS, S…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 25, 2005
    Availability
    Academic Institutions and Nonprofits only
    1624-plasmid-map-sequence-id-234

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Showing: 601 - 650 of 1735 results