1,779 results

1. pEY15

   Plasmid

   #191021

   Purpose

   flp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-1 (flp-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJW2088

   Plasmid

   #154336

   Purpose

   Crispr repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::GFP::3xFLAG::10aa linker
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJH3377

   Plasmid

   #175286

   Purpose

   Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   daf-16 d/f (daf-16 Nematode)
   

   Expression

   Worm

   Promoter

   Pges-1

   Available Since

   Nov. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. XW52

   Plasmid

   #65838

   Purpose

   XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   Czipper-QF-AD-NLS
   

   Tags

   mCherry

   Expression

   Worm

   Promoter

   Pmig-13

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCFJ2359 - intron GG2 - 250bp

   Plasmid

   #159878

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG2 - 250bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHygO2

   Plasmid

   #200077

   Purpose

   hygR selection unmarked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Expression

   Worm

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCFJ1149 - intron 3 smu-2 PATCs

   Plasmid

   #161516

   Purpose

   PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron 3 from smu-2
   

   Expression

   Worm

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEft-3::oxCerulean-oxVenus::Lgg-1

   Plasmid

   #68040

   Purpose

   Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed ubiquitously, for monitoring autophagy

   Depositor

   Dana Miller

   Article

   Chapin et al Aging (Albany NY). 2015 Jun 30.

   Insert

   pEft-3::oxCerulean-oxVenus::Lgg-1
   

   Expression

   Worm

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

   Plasmid

   #159815

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJW1838

    Plasmid

    #154324

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSR40

    Plasmid

    #69257

    Purpose

    plin-31::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    lin-31

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH4.52

    Plasmid

    #26946

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    histone H2B (his-58 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Available Since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

    Plasmid

    #182350

    Purpose

    Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBCN22-R4R3

    Plasmid

    #26302

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Tags

    His-tag

    Expression

    Worm

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSR02

    Plasmid

    #69149

    Purpose

    Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2-YFP-Smo

    Plasmid

    #31618

    Depositor

    Rajat Rohatgi

    Article

    Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

    Insert

    Smo (Smo Mouse)
    

    Use

    Zebrafish, avian expression

    Tags

    YFP

    Expression

    Mammalian and Worm

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pEY12

    Plasmid

    #191018

    Purpose

    Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    rab-3 (rab-3 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMLS286

    Plasmid

    #73723

    Purpose

    Saptrap tagRFP + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    tagRFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEY46

    Plasmid

    #191050

    Purpose

    glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    glr-1 (glr-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEY38

    Plasmid

    #191042

    Purpose

    unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    unc-8 (unc-8 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEY13

    Plasmid

    #191019

    Purpose

    Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    arrd-4 (ZK938.2 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEY20

    Plasmid

    #191026

    Purpose

    gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    gcy-18 (gcy-18 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid

    #108362

    Purpose

    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

    Depositor

    Luisa Cochella

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Inserts

    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    

    Tags

    2xFlag, MYC, T2A peptide, and TY1 linker

    Expression

    Worm

    Promoter

    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

    Plasmid

    #129538

    Purpose

    modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

    Depositor

    Arneet Saltzman

    Article

    Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox; Mos1-mediated single copy insertion (mos…

    Expression

    Worm

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGM48DEST

    Plasmid

    #74757

    Purpose

    Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

    Depositor

    Keith Yamamoto, Jordan Ward

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Inserts

    QS
    Gateway cassette
    

    Expression

    Worm

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMB62

    Plasmid

    #47944

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

    Plasmid

    #168170

    Purpose

    Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
    

    Tags

    YFP

    Expression

    Worm

    Mutation

    E497K and C566D

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAS1-rab-3P::his-24::CyOFP

    Plasmid

    #124347

    Purpose

    Nuclear Localized Pan-Neuronal CyOFP

    Depositor

    Andrew Leifer

    Insert

    rab-3, CyOFP (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJR025

    Plasmid

    #198832

    Purpose

    Actin filament and cell cortex localization.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::act2-venus::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge