1,748 results

1. 3xHA-miniTurbo_pAS32

   Plasmid

   #118222

   Purpose

   expresses 3xHA-tagged miniTurbo in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   ges-1p

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS79 (eft-3p::Cas9 + sgRNA)

   Plasmid

   #154839

   Purpose

   Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEY41

   Plasmid

   #191045

   Purpose

   dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   dat-1 (dat-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEY43

   Plasmid

   #191047

   Purpose

   eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   eat-4(prom6-1) (eat-4 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXF121

   Plasmid

   #139028

   Purpose

   MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   short mex-5 promoter (mex-5 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPD_Pinx-1::ChR2(H134R)::mCherry

   Plasmid

   #117420

   Purpose

   For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

   Depositor

   Shohei Mitani

   Article

   Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

   Insert

   channel rhodopsin-2 with H134R mutation
   

   Use

   Tags

   Expression

   Worm

   Mutation

   H134R

   Promoter

   inx-1

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEG345

   Plasmid

   #40077

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (mex-5 Nematode)
   

   Use

   Tags

   Dendra2 PFAF, S-peptide, and TEV protease cleavag…

   Expression

   Worm

   Mutation

   Promoter

   pie-1

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pHygCG2

   Plasmid

   #200073

   Purpose

   hygR selection mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

   Plasmid

   #182344

   Purpose

   sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Spacer 4
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEY11

    Plasmid

    #191017

    Purpose

    Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    Ultrapanneuronal
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pSDH54

    Plasmid

    #139052

    Purpose

    MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    tbb-2 3'UTR (tbb-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCZGY2727

    Plasmid

    #193330

    Purpose

    Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

    Depositor

    Yishi Jin

    Article

    Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

    Insert

    Hygromycin resistance
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Prps-0

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK2020

    Plasmid

    #171932

    Purpose

    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

    Depositor

    Judith Kimble

    Article

    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

    Insert

    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    

    Use

    Tags

    superfolder GFP, mCherry

    Expression

    Worm

    Mutation

    Promoter

    -

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Pmec-18 cz::caspase-3 (p17) [TU#814]

    Plasmid

    #16083

    Depositor

    Martin Chalfie

    Article

    Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

    Insert

    cz :: caspase-3 (p17) (CASP3 Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDD315 (mTurquoise2^SEC^2xHA)

    Plasmid

    #73343

    Purpose

    mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

    Insert

    mTurquoise2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans codon-optimized mTurquoise2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJJR82

    Plasmid

    #75027

    Purpose

    EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    EGFP^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized EGFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEY139

    Plasmid

    #223486

    Purpose

    tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    tph-1 (tph-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSX2719

    Plasmid

    #200763

    Purpose

    Expresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.

    Depositor

    Suhong Xu

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    MCP-24xGCN4
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    semo-1

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSX2720

    Plasmid

    #200764

    Purpose

    Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.

    Depositor

    Suhong Xu

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    scFv-sfGFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    col-19

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEY82

    Plasmid

    #191077

    Purpose

    flp-7 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-7 (flp-7 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCZGY847

    Plasmid

    #135093

    Purpose

    Transcriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegans

    Depositor

    Yishi Jin

    Article

    Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

    Insert

    Pacr-2
    

    Use

    Tags

    mcherry and unc-10 3'UTR

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TU#867 (Punc-119sid-1)

    Plasmid

    #25811

    Depositor

    Martin Chalfie

    Article

    Calixto et al Nat Methods. 2010 Jul;7(7):554-9. doi: 1

    Insert

    systemic RNA interference-deficient 1 (sid-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pRab-3::Cerulean-Venus::Lgg-1

    Plasmid

    #68045

    Purpose

    Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

    Depositor

    Dana Miller

    Article

    Chapin et al Aging (Albany NY). 2015 Jun 30.

    Insert

    pRab-3::Cerulean-Venus::Lgg-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNM3774

    Plasmid

    #154117

    Purpose

    tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆mec-7p::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSM.wrmScarlet1-10::SL2::GFP1-10

    Plasmid

    #234318

    Purpose

    For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

    Insert

    wrmScarlet1-10::SL2::GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXF89

    Plasmid

    #139030

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag (no STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. L4417

    Plasmid

    #1649

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, gfpC, gfpS65S, gfpN, T7p, lacZN, OriF1>&g…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. punc-119c

    Plasmid

    #21595

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119 (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge