1,748 results

1. pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP

   Plasmid

   #24033

   Depositor

   Cori Bargmann

   Article

   Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

   Insert

   Npr1 (npr-1 Nematode)
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJM31

   Plasmid

   #52986

   Purpose

   A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor

   Depositor

   Ding Xue

   Article

   Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

   Insert

   Annexin V (ANXA5 Human)
   

   Use

   Tags

   GFP (w/ introns) and signal sequence

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.41

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Phsp-16 cz::caspase-3 (p17) [TU#823]

   Plasmid

   #16086

   Depositor

   Martin Chalfie

   Article

   Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

   Insert

   cz :: caspase-3 (p17) (CASP3 Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK2014

   Plasmid

   #171931

   Purpose

   To express sygl-1 fused to 24 copies of MS2 loops.

   Depositor

   Judith Kimble

   Article

   Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

   Insert

   Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
   

   Use

   Tags

   N-terminal Tag 24X MS2 loops

   Expression

   Worm

   Mutation

   Promoter

   -

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMS59

   Plasmid

   #215680

   Purpose

   Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCZGY546

   Plasmid

   #90466

   Purpose

   Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pmec-4-mcherry
   

   Use

   Tags

   mcherry

   Expression

   Worm

   Mutation

   Promoter

   Pmec-4

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJR019

   Plasmid

   #198834

   Purpose

   Inactivation of G protein subunits through Pertussis toxin (PTX)

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::PTX-SL2-GFP::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSEM272 - MCS mosTI Pmlc-2_GFP target

   Plasmid

   #159834

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI Pmlc-2_GFP target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

   Plasmid

   #19326

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-3::twk-18(cn110)cDNA
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

    Plasmid

    #182352

    Purpose

    Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJR022

    Plasmid

    #198820

    Purpose

    C. elegans optimized version of mScarlet

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::wrmScarlet::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJL44 - Phsp16.48::MosTase::glh-2utr

    Plasmid

    #19333

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Phsp-16::Mos1 transposase
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJW2086

    Plasmid

    #154335

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

    Plasmid

    #193854

    Purpose

    Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' Cbr-unc-119(+) + 5' mNeonGreen
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Cbr-unc-119p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDD285

    Plasmid

    #66826

    Purpose

    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mKate2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMLS338

    Plasmid

    #73719

    Purpose

    SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Unc-32::GFP(Cbr-unc-119)
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPD96_32

    Plasmid

    #1504

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    IVSA, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, IVST,…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCZGY2850

    Plasmid

    #87758

    Purpose

    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pcol-19-PH-miniSOG(Q103L)
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Q103L

    Promoter

    Pcol-19

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXF87

    Plasmid

    #139027

    Purpose

    MosSCI backbone for MosSCI-SapTrap assembly

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPD49_83

    Plasmid

    #1448

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    hsp16-25', hsp16-415', IVSA, rf2128

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSEM255 - sgRNA mosTI Pmlc-2_GFP

    Plasmid

    #159835

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI Pmlc-2_GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOL2339

    Plasmid

    #37839

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Ub-V
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    nhx-2

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOL2340

    Plasmid

    #37841

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Ub-M
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    R77M

    Promoter

    nhx-2

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFS16 Pmyo-3 NPP-9 mChBioFLAG

    Plasmid

    #45099

    Depositor

    Steven Henikoff

    Article

    Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

    Inserts

    npp-9 (npp-9 Nematode)
    unc-119 (unc-119 Nematode)
    

    Use

    Tags

    BLRP, FLAG, and mCherry

    Expression

    Worm

    Mutation

    Promoter

    myo-3 and unc-119

    Available since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

    Plasmid

    #159853

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mNeon(PATC)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDD283

    Plasmid

    #66824

    Purpose

    YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    YPET-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized YPET

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAS3-rab-3P::AI::ChR2(H134R)

    Plasmid

    #232601

    Purpose

    Pan-neuronal expression of opsin 'ChR2(H134R)', regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    Channelrhodopsin2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

    Plasmid

    #159859

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

    Plasmid

    #182348

    Purpose

    mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge