1,748 results

1. Prab-3::NLS::GCaMP6s

   Plasmid

   #68119

   Purpose

   Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

   Depositor

   Andrew Leifer

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

   Insert

   rab-3::NLS::GCaMP6s
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPD95_77

   Plasmid

   #1495

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   IVSA, rf2204, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSF11-wNIR-GECO2-T2A-HO1

   Plasmid

   #159606

   Purpose

   Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

   Depositor

   Robert Campbell

   Article

   Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

   Insert

   wNIR-GECO2-T2A-HO1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   tag-168

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDD400

   Plasmid

   #137024

   Purpose

   SapTrap mNG-GLO donor

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   C. elegans GLO-optimized mNeonGreen
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized mNeonGreen

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJW1347

   Plasmid

   #154310

   Purpose

   Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa Linker for CT slot
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDD362

   Plasmid

   #137023

   Purpose

   SapTrap HaloTag-GLO donor

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   C. elegans GLO-optimized Halo Tag
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized Halo Tag

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAS1-rab3P::his-24::mNeptune

   Plasmid

   #124350

   Purpose

   Nuclear Localized Pan-Neuronal mNeptune

   Depositor

   Andrew Leifer

   Insert

   rab-3, mNeptune (rab-3 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGLOW77

   Plasmid

   #177338

   Purpose

   C. elegans mNeonGreen co-injection marker

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   Pmyo-2::mNeonGreen
   

   Use

   Tags

   mNeonGreen

   Expression

   Worm

   Mutation

   Promoter

   myo-2

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHKMC1: Empty sgRNA for Cloning

   Plasmid

   #67720

   Purpose

   Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

   Depositor

   Monica Colaiacovo

   Article

   Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   PU6

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAV1825

    Plasmid

    #37838

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2DsRed::KDEL
    

    Use

    Tags

    KDEL

    Expression

    Worm

    Mutation

    Promoter

    nhx-2

    Available since

    Dec. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

    Plasmid

    #19325

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Punc-122::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNM3725

    Plasmid

    #154113

    Purpose

    UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    UAS 11X::∆pes-10::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHW530 (Prab-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107131

    Purpose

    cGAL-N driver under the control of C. elegans rab-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans rab-3 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSKI

    Plasmid

    #232484

    Purpose

    pSKI can be used for single-copy transgene expression within the Universal SKI System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

    Depositor

    Carlos Silva García

    Article

    Dinneen et al bioRxiv [Preprint]. 2024 Dec 10:2024.12.

    Insert

    pSKI
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSF11-wmiRFP670-2-T2A-HO1

    Plasmid

    #197250

    Purpose

    Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP670-2-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBN267

    Plasmid

    #86723

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Prgef-1::FLP D5
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH23

    Plasmid

    #159314

    Purpose

    Punc-25 GFP::SYD-2

    Depositor

    Mei Zhen

    Article

    Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

    Insert

    syd-2 (syd-2 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Punc-25

    Available since

    Sept. 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pJW1836

    Plasmid

    #154337

    Purpose

    Promoter reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJH1579

    Plasmid

    #178904

    Purpose

    Pglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60

    Depositor

    Mei Zhen

    Article

    Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

    Insert

    YC3.60
    

    Use

    Tags

    Expression

    Worm

    Mutation

    See Depositor Comments Below

    Promoter

    Pglr-1

    Available since

    Jan. 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Pmec-18 ced-3 (p15)::nz [TU#806]

    Plasmid

    #16080

    Depositor

    Martin Chalfie

    Article

    Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

    Insert

    ced-3 (p15) :: nz (ced-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TC358

    Plasmid

    #122613

    Purpose

    TRE-delta-pes-10-GFP

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    7XTRE-delta-pes-10-GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDD363

    Plasmid

    #91829

    Purpose

    LoxP-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    LoxP-SEC-LoxP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid

    #47550

    Purpose

    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    ttTi5605 sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDD379

    Plasmid

    #91834

    Purpose

    SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pXF276

    Plasmid

    #139048

    Purpose

    MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    lov domain (includes STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJF5

    Plasmid

    #139031

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    gfp (no STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. L3829

    Plasmid

    #1604

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, lacZN

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJW1285

    Plasmid

    #61252

    Purpose

    CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting pha-1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Pmec-18 cz::ced-3 (p17) [TU#807]

    Plasmid

    #16081

    Depositor

    Martin Chalfie

    Article

    Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

    Insert

    cz:: ced-3 (p17) (ced-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJW2172

    Plasmid

    #163096

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge