8,085 results

1. EC2_1_dCas9_sgRNA

   Plasmid

   #163710

   Purpose

   Yeast low copy plasmid with dCas9 and sgRNA expression cassette

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS1NB m Rosella I

   Plasmid

   #71247

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Use

   Tags

   yeast mitochondrial targeting sequence of citrate…

   Expression

   Yeast

   Mutation

   Promoter

   PGK1

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFS255

   Plasmid

   #12382

   Depositor

   Nick Rhind

   Article

   Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

   Insert

   thymidine kinase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTEF2

   Plasmid

   #43971

   Depositor

   Hal Alper

   Article

   Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF

   Available Since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAG306-pTet07-nLuc

   Plasmid

   #199755

   Purpose

   Control construct of elongation reporter to quantify elongation of YFP

   Depositor

   Brian Zid

   Article

   Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

   Insert

   Nanoluc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCUP1-GR

   Plasmid

   #1212

   Depositor

   Susan Lindquist

   Article

   Li et al Science 2000 Jan 28;287(5453):661-4.

   Insert

   glucocorticoid receptor (Nr3c1 Rat)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

   Plasmid

   #117661

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-alphaf(I)-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPM28 (eroGFP CEN/ARS URA3)

   Plasmid

   #20131

   Depositor

   Feroz Papa

   Article

   Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

   Insert

   eroGFP (KAR2 Budding Yeast, jellyfish)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHES839

   Plasmid

   #87941

   Purpose

   Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   GAL4 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 (crippled)

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFS181

    Plasmid

    #12536

    Depositor

    Nick Rhind

    Article

    Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

    Insert

    human equilibrating nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. M3929 his3::KanMX3 Disruptor Converter

    Plasmid

    #51679

    Purpose

    Yeast marker swap plasmid for converting HIS3 to KanMX3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    KanMX3
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAR-Ec633

    Plasmid

    #130873

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L477V, L640Y, I777K, W814N

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGPD2

    Plasmid

    #43972

    Depositor

    Hal Alper

    Article

    Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GPD

    Available Since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. D1433 his3::LYS2 Disruptor Converter

    Plasmid

    #51676

    Purpose

    Yeast marker swap plasmid for converting HIS3 to LYS2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LYS2 (LYS2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRG217

    Plasmid

    #64531

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS313-PRP8

    Plasmid

    #89966

    Purpose

    complements prp8 genomic deletion

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    none

    Promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJJB1439

    Plasmid

    #218589

    Purpose

    Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    GES
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Erg20(F96W, N127W)

    Promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. NM1-5S-tRNA-SgH

    Plasmid

    #128178

    Purpose

    Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    5S rRNA-tRNA(Arg)

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. HO-Poly-KanMX4-HO

    Plasmid

    #51662

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge