8,173 results
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pKmK.P1
Plasmid#125034PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertPGK1 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBF3060
Plasmid#26853PurposeExpression of yeast codon-optimized CreA recombinase from the GAL1 promoterDepositorInsertCreA recombinase
UseCre/LoxExpressionYeastMutationN/APromoterGAL1Available SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1
Plasmid#58434PurposeInduces Citrine expression from a promoter containing 4 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)4-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
TagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426 103Q GAL
Plasmid#1188DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P12
Plasmid#125045PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertTSA1 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACT-Lex
Plasmid#183149PurposeYeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)DepositorInsertGAL4 AD
UseYeat two-hybridTagsHA tagExpressionYeastAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-Kan
Plasmid#224077PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selectionDepositorInsertymScarletI3
TagsymScarletI3ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec611
Plasmid#130872PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRepDepositorInsertTP-DNAP1
ExpressionYeastMutationI777K, L900SAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P6
Plasmid#125039PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertINU1 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P5
Plasmid#125038PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertHSP150 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P18
Plasmid#125051PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertXYL2 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P14
Plasmid#125047PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertSSA2 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P13
Plasmid#125046PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertHSP104 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P17
Plasmid#125050PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertXYL1 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P9
Plasmid#125042PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertALD4 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-EGFP
Plasmid#14204DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C1
Plasmid#125059PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS7/CenD
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
ExpressionYeastPromoterTDH3Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-TID-FKBP-HA3
Plasmid#227998PurposeExpresses TurboID-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SIVl
Plasmid#81090PurposeSingle Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179012PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)
Plasmid#179008PurposeSp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T1
Plasmid#125053PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertINU1 terminator
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
Plasmid#86413Purposenuclear split GFP11 reporter with mCherryDepositorInsertNop1pr-GFP11-mCherry-PUS1
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREP3-TEV-NLS
Plasmid#21146DepositorInsertTEV protease (TEVgp1 tobacco etch virus)
UseS. pombeTags2xNLS, NLS, and mycExpressionYeastAvailable SinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGal-BE3
Plasmid#172409PurposeYeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoterDepositorInsertBE3
UseCRISPRExpressionYeastPromoterGALLAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and HXT7Available SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSN068
Plasmid#101749PurposeSingle copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastPromoterHeterologous TEF1 promoter from A. gossypii. and …Available SinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P15
Plasmid#125048PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertTDH3 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
TagsHA tag and myc tagExpressionYeastPromoterGAL1Available SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C2
Plasmid#125060PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS/CEN5
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T5
Plasmid#125057PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertPGK1 terminator
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-H
Plasmid#222846PurposeYeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-H
ExpressionYeastAvailable SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_gRNA_handle_RPR1t
Plasmid#49014PurposegRNA expression empty vector (yeast)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastPromoterpRPR1Available SinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P16
Plasmid#125049PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertFBA1 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSJ95
Plasmid#104343PurposeExpress yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertFBP1
Tags3xFLAGExpressionYeastAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only
