8,010 results

1. pPOM033_P7_3"His5

   Plasmid

   #216458

   Purpose

   3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   3''his5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPOM026_P2_pNMT1

   Plasmid

   #216451

   Purpose

   Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter nmt1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPOM019_P2_pRPL2102

   Plasmid

   #216444

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter rpl2102
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPOM015_P2_pADH1

   Plasmid

   #216440

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter adh1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPOM013_P2_pENO101

   Plasmid

   #216438

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter eno101
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCEV-G2-Km ymScarletI

   Plasmid

   #193960

   Purpose

   TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymScarletI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MAP72_MS2

   Plasmid

   #220623

   Purpose

   Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Type

   Empty backbone

   Use

   Shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-hygMV

   Plasmid

   #73891

   Purpose

   fission yeast drug selection marker

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Insert

   hygMV
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pombe TEF

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ZJOM113

   Plasmid

   #133666

   Purpose

   Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJZC588

    Plasmid

    #62315

    Purpose

    sgRNA with 2x MS2 (wt+f6) for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x MS2 (wt+f6) binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YEp181-CUP1-His-Smt3

    Plasmid

    #99536

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG425GPD-ccdB-EGFP

    Plasmid

    #14202

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXZX351

    Plasmid

    #169454

    Purpose

    pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATα
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRR-GR-5Z

    Plasmid

    #23060

    Depositor

    Charles Miller

    Article

    Miller et al Plasmid. 2009 Dec 3. ():.

    Insert

    GR (NR3C1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

15. YIplac211-VPS10-msGFPx3

    Plasmid

    #105274

    Purpose

    Plasmid for pop-in/pop-out replacement of Vps10 with Vps10-msGFPx3.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Vps10 portion (PEP1 Budding Yeast)
    

    Use

    Tags

    msGFPx3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYZ294

    Plasmid

    #102688

    Purpose

    S. pombe gRNA entry vector with Cas9 - KanMX marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYZ300

    Plasmid

    #102689

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp his3 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHLU

    Plasmid

    #64167

    Purpose

    harbours marker genes HIS3, LEU2 and URA3

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BS-Ura3Kl

    Plasmid

    #69195

    Purpose

    complementation of yeast auxotrophic marker URA3(Kluyveromyces lactis)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Kluyveromyces lactis URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJAID5XY

    Plasmid

    #165051

    Purpose

    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS406 ATG9 stopGFP

    Plasmid

    #114669

    Purpose

    Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Changed the three linker base pairs after the las…

    Promoter

    ATG9

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLexA

    Plasmid

    #11342

    Depositor

    Guy Caldwell

    Type

    Empty backbone

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pMJA103

    Plasmid

    #128519

    Purpose

    Expresses human amyloid Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme D67H (LYZ Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB2196

    Plasmid

    #67549

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG303-Gal-GA50

    Plasmid

    #84907

    Purpose

    induce expression of dipeptide repeat GA in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    GA50 (C9orf72 Synthetic)
    

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJW1664

    Plasmid

    #112038

    Purpose

    CEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HIS

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    Su9
    

    Use

    Tags

    TagBFP

    Expression

    Yeast

    Mutation

    amino acids 1-76

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pI1-MTU-DO-HPH

    Plasmid

    #160014

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKmK.C1

    Plasmid

    #125059

    Purpose

    Storage plasmid for K.marxianus centromere

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    KmARS7/CenD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBD-pZ-FLAG

    Plasmid

    #183148

    Purpose

    Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 BD
    

    Use

    Yeast two-hybrid

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pH3FS

    Plasmid

    #85780

    Purpose

    N-ICE plasmid pH3FS

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTUP1

    Plasmid

    #104341

    Purpose

    Express TUP1

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

    Insert

    TUP1 (TUP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ZJOM5

    Plasmid

    #133635

    Purpose

    Integration of CHS5-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-CHS5-GFP (CHS5 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3

    Plasmid

    #203657

    Purpose

    Yeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Tags

    GFPMUT3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG306GPD-ccdB-DsRed

    Plasmid

    #14356

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Gal1/10p CAS9 Gal1/10p I-SceI

    Plasmid

    #182519

    Purpose

    Galactose-Induced Expression vector for Cas9 and I-SceI.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Inserts

    I-SceI
    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGI3EM20C

    Plasmid

    #135487

    Purpose

    Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr H2B-tdTomato
    

    Use

    Tags

    tdTomato

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFA6a-6xGLY-His-tag-hphMX4

    Plasmid

    #20764

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSP-G2

    Plasmid

    #64737

    Purpose

    Yeast expression plasmid

    Depositor

    Jens Nielsen

    Article

    Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFA6a-HyTkAX

    Plasmid

    #73898

    Purpose

    fission yeast drug counter-selectable selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    hyg-TK
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p416 46Q GPD

    Plasmid

    #1178

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 46Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only