8,350 results

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pRS405 VAC8-GFP

Plasmid

#166836

Purpose

Expresses Vac8-GFP in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-URA

Plasmid

#160015

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-LEU

Plasmid

#160013

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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plIVMD156

Plasmid

#160934

Purpose

In vivo mRNA display Gateway Destination construct

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAG303GAL-ccdB-EGFP

Plasmid

#14181

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS-RRM Mutant-YFP

Plasmid

#29608

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS RRM mutant (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

F305L, F341L, F359L and F368L

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
YEpURA-CUP1-HBTubiquitin

Plasmid

#26900

Depositor

Peter Kaiser

Article

Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

Insert

HBT-tagged ubiquitin

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pDRFLIP34

Plasmid

#65512

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7TFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSB305-K3L-vac

Plasmid

#20053

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-EGFP-ccdB

Plasmid

#14312

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pJK59-ATG17-GFP

Plasmid

#44175

Depositor

James Hurley

Article

Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

Insert

Atg17 (ATG17 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

none

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
p413TEF-Sir2-H364Y

Plasmid

#51743

Purpose

overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Expression

Yeast

Mutation

H364Y

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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GAL 72Q+ProGFPp416

Plasmid

#15582

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q + Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pPS1372

Plasmid

#1353

Depositor

Pamela Silver

Article

Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

Insert

pADH-NLS-NES-GFP2

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pDRF1 Cyterm-ytd8oxStayGold

Plasmid

#219853

Purpose

expression of Cyterm-ytd8oxStayGold in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

Cyterm-ytd8oxStayGold

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTR1-GPD-TRPC

Plasmid

#190103

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

Depositor

Perng-Kuang Chang

Article

Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAGE2.0-i

Plasmid

#194639

Purpose

Empty pAGE2.0 plasmid with pBAD inducible promoter

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBEVY-4420-mCherry

Plasmid

#218828

Purpose

Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

4-4-20 scFv

Expression

Yeast

Promoter

GAL1-10

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRL_yEGFP_hc

Plasmid

#98746

Purpose

Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

Insert

Yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Promoter

synthetic

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGALSc104(A503V)

Plasmid

#1152

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

A503V

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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RS314-H

Plasmid

#17541

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Expression

Yeast

Mutation

R273H

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pDRf1-4CL5-AtSCT (JBEI-11557)

Plasmid

#87937

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSCT

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

AtSCT
4CL5 (4CL5 Mustard Weed)

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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PRS303-Gal1pr-Mag1-sctetR

Plasmid

#44753

Depositor

Narendra Maheshri

Article

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

MAG1-sctetR

Expression

Yeast

Promoter

Gal1pr

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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M3926 leu2::KanMX3 Disruptor Converter

Plasmid

#51680

Purpose

Yeast marker swap plasmid for converting LEU2 to KanMX3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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YIplac204-T|C-iGFP-PH(OSH1)

Plasmid

#105280

Purpose

Overexpresses iGFP-PH(Osh1) under the TPI1 promoter. Integrating at TRP1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

PH domain of Osh1 (SWH1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Promoter

TPI1

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymStayGold-T2A-mCherry

Plasmid

#219843

Purpose

equimolar expression of ymStayGold and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymStayGold-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJZC506

Plasmid

#62281

Purpose

COM-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

COM-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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p405CYC1

Plasmid

#15967

Depositor

Nicolas Buchler, Fred Cross

Insert

p405CYC1 (CYC1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

CYC1 promoter is between SacI and XbaI restrictio…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pUDE710

Plasmid

#103020

Purpose

expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Kanamycin

Plasmid

#188933

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Kanamycin

Expression

Yeast

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLUC

Plasmid

#1280

Depositor

Susan Lindquist

Article

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

CUP1-lacZ fusion (CUP1-1 Budding Yeast)

Tags

CUP1 promoter

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pHES875

Plasmid

#89192

Purpose

Expresses mCherry under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-mCherry

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR

Plasmid

#60619

Purpose

crRNA expression plasmid on pRS423 backbone

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRR2

Plasmid

#166097

Purpose

Gal inducible expression of dCas9-FokI

Depositor

Rebecca Burgess

Insert

dCas9-FokI

Use

CRISPR

Expression

Yeast

Mutation

spCas9-D10A, H840A

Promoter

Gal-inducible

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRf1-4CL5-DsRed (JBEI-15461)

Plasmid

#87935

Purpose

Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Tags

6xHA

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCT-4M5.3

Plasmid

#41844

Depositor

Dane Wittrup

Article

Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

Insert

4M5.3

Tags

HA and c-myc

Expression

Yeast

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG413GAL-ccdB-EGFP

Plasmid

#14189

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomSua1

Plasmid

#107446

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sua1

Tags

V5-His

Expression

Yeast

Mutation

See depositor comments below.

Promoter

GAL1

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomSir1

Plasmid

#107447

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sir1 (sir1 Fission Yeast)

Tags

V5-His

Expression

Yeast

Mutation

H523R, A750T

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomMet1

Plasmid

#107444

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met1

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
RS314-SN

Plasmid

#17539

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Expression

Yeast

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Article

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pGAL - FFL[SEL]

Plasmid

#1102

Depositor

Susan Lindquist

Article

Singer et al Mol Cell 1998 Apr;1(5):639-48.

Insert

firefly luciferase

Expression

Yeast

Mutation

n/a

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pDH22

Plasmid

#83798

Purpose

Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pXP841

Plasmid

#46067

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac211-GGA2-msGFPx3

Plasmid

#105258

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Gga2 portion (GGA2 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4046664

Plasmid

#149224

Purpose

Yeast Expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046660

Plasmid

#149214

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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