8,265 results
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Plasmid#105258PurposePlasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCT-4M5.3
Plasmid#41844DepositorInsert4M5.3
TagsHA and c-mycExpressionYeastAvailable SinceJan. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR
Plasmid#60619PurposecrRNA expression plasmid on pRS423 backboneDepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterSNR52Available SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEDJ400
Plasmid#177269PurposeUSER cloning-compatible 2μ vector (URA3)DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0710
Plasmid#133517PurposeExpression of mCherry tagged beta-tubulin to monitor microtubulesDepositorInsertpAde6(PmeI)-p(atb2)-mCherry-atb2-terminator(atb2)-hphMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mTurquoise2
Plasmid#125701PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2DepositorInsertymNeongreen-T2A-mTurquoise
ExpressionYeastAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-Cerulean
Plasmid#14396DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAV113
Plasmid#63180PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
G31, Cdc20-AID tagging
Plasmid#130269PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombinationDepositorInsertCdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
TagsAID(71-114)ExpressionYeastPromoterNative promoterAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP841
Plasmid#46067DepositorTypeEmpty backboneExpressionYeastPromoterADH2Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAC690-TEF
Plasmid#160432PurposeExpresses eGFP under control of the yeast TEF2 promoterDepositorInsertS. cerevisiae TEF2 promoter
ExpressionYeastPromoterS. cerevisiae TEF2Available SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1
Plasmid#159166PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1
TagsSV40 NLSExpressionYeastPromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRFLIP37
Plasmid#65515PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-Cer-cMycExpressionYeastPromoterpPMA1Available SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAL - FFL[SEL]
Plasmid#1102DepositorInsertfirefly luciferase
ExpressionYeastMutationn/aAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG425GAL-EGFP-ccdB
Plasmid#14321DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUDP012
Plasmid#101167PurposeE. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
ExpressionBacterial and YeastPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB170
Plasmid#158600PurposeTranscription factors ADR1c, PIP2c, OAF1cDepositorInsertsADR1c
PIP2c
OAF1c
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)
Plasmid#179054PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubulesDepositorInsertRPL18Bp-Scarlet(rfp)-Link-TUB1
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TC-OM45-FKBPx4
Plasmid#104472PurposeIntegrating plasmid for overexpression of OM45-FKBPx4.DepositorAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT11F
Plasmid#165062PurposeIntroducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
ExpressionYeastMutationWTAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaARG4
Plasmid#162463PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4DepositorInsert3FLAG-Mnase
ExpressionYeastAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG
Plasmid#176400PurposeExpresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.DepositorInsertFRB-RGG-GFP-RGG-RGG
TagsFRB and GFPExpressionYeastPromoterGAL1Available SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
416GPD-FUS-YFP
Plasmid#29594DepositorAvailable SinceJuly 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKL1-222
Plasmid#219409PurposeNuclear expression of Synrophin PDZ clamp proteinDepositorInsertpTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
ExpressionYeastAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046538
Plasmid#149128PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpTEF1Available SinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB4848
Plasmid#106138PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP459
Plasmid#85933PurposeEncodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.DepositorInsertAnti-Ebola 2G4 monoclonal antibody
ExpressionYeastPromoterAOX1Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3GFP-kanMX6
Plasmid#105148PurposeYeast gene targetingDepositorInsert3GFP-kanMX6
Tags3GFPExpressionBacterial and YeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETCON-TNF
Plasmid#67748PurposeTumor Necrosis Factor Alpha Yeast Display PlasmidDepositorInsertTNF-Alpha
ExpressionBacterial and YeastAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
LD201
Plasmid#69178PurposeIntegration of 2GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg1-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCUP1pNuiHA kanMX CEN
Plasmid#131168Purposefor ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneTagsNui-HAExpressionYeastAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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p416-GPD-PRUNE-HA
Plasmid#183945PurposeExpresses human HA-tagged PRUNE from yeast GPD promoterDepositorAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plp
Plasmid#22310DepositorTypeEmpty backboneUseIntegrating plasmidExpressionYeastAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG304GPD-ccdB
Plasmid#14136DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM141
Plasmid#133669PurposeIntegration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-UBQLN2
Plasmid#181708PurposeGalactose inducible expression of UBQLN2DepositorInsertUBQLN2 (UBQLN2 Human)
ExpressionYeastAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS423-TyrSgH
Plasmid#163973PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoterDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET16
Plasmid#107442PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415PGK1-AtMAX1(pYL759)
Plasmid#178289PurposeExpresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5DepositorInsertAtMAX1
ExpressionYeastPromoterPGK1Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-hENT1
Plasmid#166028PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyExpressionYeastPromoterpPGK1Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-GOS1
Plasmid#18846DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-neoCV
Plasmid#73889Purposefission yeast drug selection markerDepositorInsertneoCV
ExpressionBacterial, Mammalian, and Y…PromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP36
Plasmid#65514PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionYeastPromoterpPMA1Available SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
ExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG49
Plasmid#164707PurposeyEVenus under the control of GAL1 promoterDepositorInsertGAL1pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only