8,173 results
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Plasmid#52224PurposeExpression of sgRNA precursor with CspCI placeholder for target cloning (see comments).DepositorInsertrrk1-sgRNA
UseCRISPRExpressionYeastPromoterrrk1Available SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-Kanamycin
Plasmid#188933PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-Kanamycin
ExpressionYeastAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG303GPD-ccdB-EGFP
Plasmid#14182DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG25QDProCFPp303
Plasmid#15584DepositorInserthtt 25Q Delta Pro (HTT Human)
TagsCFP and FLAGExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM96
Plasmid#133663PurposeIntegration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI2
Plasmid#176156Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ytdTomato-CaURA3
Plasmid#118461PurposeYeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.DepositorInsertytdTomato
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymVenus-URA3
Plasmid#168058PurposeReplaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymVenus
TagsymVenusExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHES822
Plasmid#89193PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
ExpressionYeastPromoterpZAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 FL
Plasmid#163574Purposeexpress wild type Dcp2 at endogenous level in S.cerevisiaeDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV105
Plasmid#63195PurposepRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PP74
Plasmid#78944PurposeSupplemental Figure 3. Integration Site 1.DepositorInsertIntegration Site 1
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-K2-L2
Plasmid#80684PurposeGateway Entry vector containing a temperature-sensitive mouse DHFRDepositorUseSynthetic BiologyTags3xHAExpressionBacterial, Insect, Mammalia…Mutationmouse DHFR with N-terminal UbiquitinAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPM48
Plasmid#22854DepositorInserteroGFP & UPR-RFP
ExpressionYeastAvailable SinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
ZJOM100
Plasmid#133662PurposeIntegration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LHP398 - Ub-K48R/K63R
Plasmid#32167DepositorExpressionYeastMutationK48R and K63RPromoterCUP1Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMAL498
Plasmid#169154PurposeEncodes OptoQ-INVRT4DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dMFot
Plasmid#44558DepositorInsertsPTETREG promoter
rtetR-M2::FFF
UseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationCompared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…PromoterPTETREGAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gma-pCAMBIA-1301-STTM171
Plasmid#84080PurposeDisturb the expression of miR171 in soybeanDepositorInsertmiR171
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT25_GalL_BE3
Plasmid#143736PurposeExpresses BE3 in yeast cellsDepositorInsertBE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT82_GalL_nCDA1Δ198-BE3
Plasmid#145046PurposeExpresses nCDA1Δ198-BE3 in yeast cellsDepositorInsertnCDA1Δ198-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-198AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal G294A YFP
Plasmid#27448DepositorInsertTDP-43 (TARDBP Human)
TagsYFPExpressionYeastMutationGlycine 294 mutated to Alanine (G294A)Available SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046664
Plasmid#149224PurposeYeast Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046660
Plasmid#149214PurposeYeast Expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046689
Plasmid#149212PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPTK006-3a-αMF no EAEA
Plasmid#84965PurposeType 3a, α-mating factor no EAEA - secretion signalDepositorInsertα-mating factor no EAEA secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)
Plasmid#178989PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1010
Plasmid#111423PurposegRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)DepositorInsertsgADE-NT2
UseCRISPRExpressionYeastAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG46QDProGFP p306
Plasmid#15832DepositorInserthtt 46Q Delta Pro (HTT Human)
TagsFLAG and GFPExpressionYeastMutationProline-rich region removedAvailable SinceOct. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
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LHP2260 - HA-INP53
Plasmid#32198DepositorAvailable SinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-TAF15
Plasmid#181713PurposeGalactose inducible expression of TAF15DepositorInsertTAF15 (TAF15 Human)
ExpressionYeastAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-hnRNPA1
Plasmid#181710PurposeGalactose inducible expression of hnRNPA1DepositorInserthnRNPA1 (HNRNPA1 Human)
ExpressionYeastAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRA03
Plasmid#103141PurposeTriple gRNA expression separated with 28nt Csy4 motifDepositorInsertgRNAs
ExpressionYeastAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mCFP-kanMX6
Plasmid#105145PurposeYeast gene targetingDepositorInsertmCFP-kanMX6
TagsmCFPExpressionBacterial and YeastMutationA206KAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJJH1295
Plasmid#36914DepositorInsert3x mCherry
ExpressionBacterial, Mammalian, and Y…Available SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179051PurposeVector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolusDepositorInsertRPL18Bp-RPC19-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Ph
Plasmid#46818PurposeTEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and GAL10Available SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only