8,016 results

1. pAG416GAL-3x-uC-ccdB-6stop

   Plasmid

   #203514

   Purpose

   Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG416GAL10-ccdB-6stop

   Plasmid

   #203512

   Purpose

   Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG416GAL-1x-ccdB-6stop

   Plasmid

   #203519

   Purpose

   Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG416GAL-2x-uA-ccdB-6stop

   Plasmid

   #203517

   Purpose

   Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG416GAL-DENV_NS3-GS-NS2B

   Plasmid

   #201940

   Purpose

   Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   DENV NS3-GS-NS2B (POLY )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG416GALL-HIV-1_PR

   Plasmid

   #203493

   Purpose

   Expresses HIV-1 protease from a GALL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   HIV-1 protease
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GALL

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG416GAL10-WNV_NS2B-GS-NS3

   Plasmid

   #203475

   Purpose

   Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   WNV NS2B-GS-NS3 (POLY )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG426GAL-SARS-CoV-2_PLP

   Plasmid

   #203509

   Purpose

   Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   SARS-CoV-2 PLP (ORF1ab )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG426GAL-KUNV_NS2B-GS-NS3

   Plasmid

   #203506

   Purpose

   Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   KUNV NS2B-GS-NS3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pE1c

    Plasmid

    #17461

    Purpose

    modified pENTR vector for C-terminal STREP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    STREP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG414GPD-EGFP-ccdB

    Plasmid

    #14312

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pV1435

    Plasmid

    #111439

    Purpose

    Solo vector pV1382 + sgCgADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgCgADE2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV0710

    Plasmid

    #133517

    Purpose

    Expression of mCherry tagged beta-tubulin to monitor microtubules

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(atb2)-mCherry-atb2-terminator(atb2)-hphMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMC-X4

    Plasmid

    #176154

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC-XII1

    Plasmid

    #176159

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMC-XI3

    Plasmid

    #176157

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS403red-­luxTA2-­4F

    Plasmid

    #166030

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS405red-­phlTA2-­1E

    Plasmid

    #166029

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GAL 25Q+ProGFPp416

    Plasmid

    #15580

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q + Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pPOP

    Plasmid

    #59526

    Purpose

    Vector for pop-in/pop-out gene replacement in Pichia pastoris.

    Depositor

    Benjamin Glick

    Article

    Soderholm et al Biotechniques. 2001 Aug;31(2):306-10, 31

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAG423GPD-ccdB-HA

    Plasmid

    #14246

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDH22

    Plasmid

    #83798

    Purpose

    Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-12Pk-LEU2MX6

    Plasmid

    #49179

    Purpose

    genomic 12Pk epitope tagging in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    12Pk

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA-MNase-CaARG4

    Plasmid

    #162463

    Purpose

    DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4

    Depositor

    Adnane Sellam

    Article

    Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

    Insert

    3FLAG-Mnase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJZC519

    Plasmid

    #62278

    Purpose

    Yeast dCas9-VP64 expression plasmid

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    dCas9-VP64
    

    Use

    Tags

    Has a VP64 fusion

    Expression

    Yeast

    Mutation

    Has mutations in the RuvC1 and HNH domains to ren…

    Promoter

    pTdh3

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPS811

    Plasmid

    #8904

    Depositor

    Pamela Silver

    Article

    Lee et al Genes Dev 1996 May 15;10(10):1233-46.

    Insert

    NPL3 (NPL3 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pICE HIS3

    Plasmid

    #122877

    Purpose

    HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK59-ATG17-GFP

    Plasmid

    #44175

    Depositor

    James Hurley

    Article

    Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

    Insert

    Atg17 (ATG17 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    none

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pFA6a-link-yoClover-Kan

    Plasmid

    #44952

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized Clover with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoClover
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. plIVMD156

    Plasmid

    #160934

    Purpose

    In vivo mRNA display Gateway Destination construct

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
    

    Use

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGBW-m4046323

    Plasmid

    #149100

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pESC-LEU-PmCDA1

    Plasmid

    #60818

    Purpose

    to overexpress lamprey deaminase in yeast

    Depositor

    Youri Pavlov

    Article

    Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

    Insert

    CDA1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCfB3034(X-3 MarkerFree)

    Plasmid

    #73272

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pREP3-TEV-NLS

    Plasmid

    #21146

    Depositor

    Stephen Kearsey

    Article

    Yang et al BMC Mol Biol. 2005 . 6():13.

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Use

    S. pombe

    Tags

    2xNLS, NLS, and myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAV0663

    Plasmid

    #133478

    Purpose

    Expression in fission yeast from promoter pact1, integrated at his5 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRS416-PHO5-GFP-Opi1 + terminator

    Plasmid

    #58836

    Purpose

    Expresses GFP-Tagged Opi1 + Terminator in yeast

    Depositor

    Tim Levine

    Insert

    Opi1p (OPI1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

37. PhyB-mCherry-CDC10

    Plasmid

    #51568

    Purpose

    Constitutively express bud neck localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    CDC10 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPTK003-2-pENO1

    Plasmid

    #84962

    Purpose

    Type 2, pENO1, Enolase 1 promoter

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Enolase 1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    BsaI site removed (2350 g to c) and BsmBI site re…

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid

    #74368

    Purpose

    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    yeGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGRR-r11-r10

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG415GPD-ccdB-HA

    Plasmid

    #14242

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge