Skip to main content
Addgene

8,021 results

Showing: 2041 - 2080 of 8021 results
  1. pUDE735

    Plasmid
    #103024
    Purpose
    expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)
    Depositor
    Insert
    crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SNR52
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pDEST-N2H-F1-empty vector

    Plasmid
    #125551
    Purpose
    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)
    Depositor
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. p42Neo 1-F GW

    Plasmid
    #58550
    Purpose
    Multicopy with G418 resistance marker for single Gateway insertion
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. Linked Free NES

    Plasmid
    #182485
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. Linked Free PcPTS

    Plasmid
    #182486
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Insert
    FPPS-PTS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pSK267

    Plasmid
    #129272
    Purpose
    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein
    Depositor
    Insert
    NLS-VP16-ZF 43-8-NES
    Use
    Tags
    6xHA
    Expression
    Yeast
    Mutation
    Promoter
    SUP35
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pMZ288

    Plasmid
    #59897
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+
    Depositor
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. p405CYC1

    Plasmid
    #15967
    Insert
    p405CYC1 (CYC1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    CYC1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
  9. APEX2 VLP

    Plasmid
    #182211
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).
    Depositor
    Inserts
    VP2C-APEX2
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology; Tem imaging
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pRL_yEGFP_hc

    Plasmid
    #98746
    Purpose
    Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.
    Insert
    Yeast-enhanced green fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    synthetic
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. FRP2000

    Plasmid
    #159309
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selection
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pGAL-SKI1-NAT

    Plasmid
    #160382
    Purpose
    Overxpression of SKI1/XRN1 in yeast
    Depositor
    Insert
    SKI1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pPt_Cp

    Plasmid
    #206855
    Purpose
    A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pCT

    Plasmid
    #60620
    Purpose
    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
    Depositor
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  15. pGBW-m4046348

    Plasmid
    #149108
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp6
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  16. pFA6a-CLIP-KanMX6

    Plasmid
    #87025
    Purpose
    Tag S. pombe genes in C terminal with CLIP
    Depositor
    Insert
    CLIP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  17. pGBW-m4046451

    Plasmid
    #145567
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp6
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  18. pAV113

    Plasmid
    #63180
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2041 - 2080 of 8021 results