8,021 results
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Plasmid#103024Purposeexpression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)DepositorInsertcrCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-F1-empty vector
Plasmid#125551PurposeN2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 alone without any attached prot…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS423-FjTAL(JBEI-15466)
Plasmid#87936PurposeExpression in S.cerevisiae of FjTALDepositorInsertFjTAL
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p42Neo 1-F GW
Plasmid#58550PurposeMulticopy with G418 resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET16
Plasmid#107442PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET16 (MET16 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mNeongreen-T2A-mCherry
Plasmid#125698PurposeProduces equimolar expression of mNeongreen and mCherryDepositorInsertmNeongreen-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free PcPTS
Plasmid#182486PurposeYeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS-PTS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p415PGK1-AtMAX1(pYL759)
Plasmid#178289PurposeExpresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5DepositorInsertAtMAX1
UseTagsExpressionYeastMutationPromoterPGK1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVG49
Plasmid#164707PurposeyEVenus under the control of GAL1 promoterDepositorInsertGAL1pr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSK267
Plasmid#129272Purpose"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed proteinDepositorInsertNLS-VP16-ZF 43-8-NES
UseTags6xHAExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-GGA2-msGFPx3
Plasmid#105258PurposePlasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.DepositorInsertGga2 portion (GGA2 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXP222
Plasmid#26833DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-Purg1-3FLAG
Plasmid#19355DepositorInserturg1 promoter
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMZ288
Plasmid#59897PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
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APEX2 VLP
Plasmid#182211PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRL_yEGFP_hc
Plasmid#98746PurposeReporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.DepositorInsertYeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationPromotersyntheticAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YL-CashGem-BAT1-URA3-LINEAR
Plasmid#174837PurposepRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolyticaDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterGPDpAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRP2000
Plasmid#159309PurposeYeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-RmMYB108
Plasmid#173183PurposeExpresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.DepositorInsertRmMYB108
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJZC504
Plasmid#62280PurposePCP-VP64 fusionDepositorInsertPCP-VP64
UseTagsVP64ExpressionYeastMutationPromoterpAdhAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAL-SKI1-NAT
Plasmid#160382PurposeOverxpression of SKI1/XRN1 in yeastDepositorInsertSKI1
UseTagsExpressionYeastMutationPromoterGALAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ENT5-HaloTag
Plasmid#177102PurposeExpresses Ent5-HaloTag in yeast cellsDepositorInsertENT5 (ENT5 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
416GPD-FUS-YFP
Plasmid#29594DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJuly 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPt_Cp
Plasmid#206855PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNB783
Plasmid#60162PurposeFirefly luciferase (Promega) driven by RNR1 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationA4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Kan
Plasmid#188918PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Kan
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mTurquoise2
Plasmid#125701PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2DepositorInsertymNeongreen-T2A-mTurquoise
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT
Plasmid#60620PurposePlasmid encoding iCas9 and tracrRNA on pRS415 backboneDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046348
Plasmid#149108PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneUseTagsGal4 Activation domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-CLIP-KanMX6
Plasmid#87025PurposeTag S. pombe genes in C terminal with CLIPDepositorInsertCLIP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEL12
Plasmid#107918Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046451
Plasmid#145567PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV113
Plasmid#63180PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G11-5FLAG-kanMX6
Plasmid#49182Purposegenomic G11-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneUseTagsG11-5FLAGExpressionYeastMutationPromoterAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SYS1-msGFPx3
Plasmid#177112PurposeExpresses Sys1-msGFP3 in yeast cellsDepositorInsertSYS1 (SYS1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -